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Book Cover

Title Cardiovascular proteomics : methods and protocols / edited by Fernando Vivanco
Published Totowa, N.J. : Humana Press, ©2007


Description 1 online resource (xv, 420 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; 357
Methods in molecular biology (Clifton, N.J.) ; v. 357
Contents Two-Dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomics / Michael J. Dunn -- Analyzing the Cardiac Muscle Proteome by Liquid Chromatography-Mass Spectrometry-Based Expression Proteomics / Anthony O. Gramolini [and others] -- Two-Dimensional Differential Gel Electrophoresis of Rat Heart Proteins in Ischemia and Ischemia-Reperfusion / Jun Sakai [and others] -- Analysis of Antihypertensive Drugs in the Heart of Animal Models: A Proteomic Approach / Alberto Lázaro [and others] -- A Solubility Optimization Protocol For Two-Dimensional Gel Electrophoresis of Cardiac Tissue / Brian A. Stanley and Jennifer E. Van Eyk -- A Method for Effective Depletion of Albumin From Cellular Extracts: Application to Human Myocardium / Brian A. Stanley and Jennifer E. Van Eyk -- Subcellular Fractionation / Mariana Eca Guimarães de Araújo and Lukas Alfons Huber -- Subfractionation of Heart Tissue: The "In Sequence" Myofilament Protein Extraction of Myocardial Tissue / Lesley A. Kane, Irina Neverova, and Jennifer E. Van Eyk -- Optimization of Cardiac Troponin I Pull-Down by IDM Affinity Beads and SELDI / Diane E. Bovenkamp, Brian A. Stanley, and Jennifer E. Van Eyk -- Proteomic Analysis of the Subunit Composition of Complex I (NADH: Ubiquinone Oxidoreductase) From Bovine Heart Mitochondria / Ian M. Fearnley, Joe Carroll, and John E. Walker -- Identification of Targets of Phosphorylation in Heart Mitochondria / Roberta A. Gottlieb -- Characterization of the Human Atheroma Plaque Secretome by Proteomic Analysis / Mari-Carmen Durán [and others] -- Characterization of HSP27 Phosphorylation Sites in Human Atherosclerotic Plaque Secretome / Mari-Carmen Durán [and others] -- Western Array Analysis of Human Atherosclerotic Plaques / Wim Martinet
The Proteome of Endothelial Cells / Jesús González-Cabrero [and others] -- Proteomic Study of Caveolae and Rafts Isolated From Human Endothelial Cells / Richard R. Sprenger and Anton J.G. Horrevoets -- Proteomic Identification of S-Nitrosylated Proteins in Endothelial Cells / Antonio Martínez-Ruiz and Santiago Lamas -- The Proteome and Secretome of Human Arterial Smooth Muscle Cell / Annabelle Dupont and Florence Pinet -- Real-Time In Vivo Proteomic Identification of Novel Kinase Substrates in Smooth Muscle Cell / Anne A. Wooldridge and Timothy A. Haystead -- Proteomic Analysis of Vascular Smooth Muscle Cells Treated With Ouabain / Alexey V. Pshezhetsky -- Isolation and Culture of Adult Mouse Cardiac Myocytes / Timothy D. O'Connell, Manoj C. Rodrigo, and Paul C. Simpson -- Proteomic Analysis of Foam Cells / Peng-Yuan Yang [and others] -- Isolation of the Platelet Releasate / Judith Coppinger, Desmond J. Fitzgerald, and Patricia B. Maguire -- Enrichment of Phosphotyrosine Proteome of Human Platelets by Immunoprecipitation / Martina Foy, Donal F. Harney, Kieran Wynne, and Patricia B. Maguire -- Characterization of Circulating Human Monocytes by Proteomic Analysis / Maria G. Barderas [and others] -- Cardiovascular Biomarker Discovery by SELDI-TOF Mass Spectrometry / Olivier Meilhac, Sandrine Delbosc, and Jean-Baptiste Michel -- Detection of Biomarkers of Stroke Using SELDI-TOF / Jean-Charles Sanchez [and others] -- Depletion of High-Abundance Proteins in Plasma by Immunoaffinity Substraction for Two-Dimensional Difference Gel Electrophoresis Analysis / Verónica M. Dardé, Maria G. Barderas, and Fernando Vivanco -- A Rapid, Economical, and Reproducible Method For Human Serum Delipidation and Albumin and IgG Removal For Proteomic Analysis / Qin Fu, Diane E. Bovenkamp, and Jennifer E. Van Eyk -- Mitoproteome: Human Heart Mitochondrial Protein Sequence Database / Purnima Guda, Shankar Subramaniam, and Chittibabu Guda -- Techniques to Decipher Molecular Diversity by Phage Display / Dawn R. Christianson [and others] -- Index
Summary Over the past few years, the power and potential of proteomics has become widely recognized. The use of proteomics for the study of complex diseases is increasing and is particularly applicable to cardiovascular disease, the leading cause of death in developed countries. The ability to investigate the complete proteome provides a critical tool toward elucidating the complex and multif- torial basis of cardiovascular biology, especially disease processes. Proteomics involves the integration of a number of technologies with the aim of analyzing all the proteins expressed by a biological system in response to various stimuli under different pathophysiological conditions. The proteomic approach offers the ability to evaluate simultaneously the changes in protein expression and cell signaling pathways in response to such conditions as atherosclerosis, c- diac hypertrophy, stroke, or heart failure. Cardiovascular Proteomics: Methods and Protocols covers many of the above aspects of the proteomic approach in the cardiovascular field. This v- ume takes the reader through the complete process of proteomic analysis, from the obtention of specific heart proteins (troponin I) to the new techniques of identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, or the application of phage display techniques to decipher the molecular diversity of blood vessels
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Cardiovascular system -- Diseases -- Molecular aspects
Biochemical markers.
Two-dimensional electrophoresis.
Proteomics -- methods
Biomarkers -- analysis
Electrophoresis, Gel, Two-Dimensional -- methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization -- methods
Electrophoresis, Gel, Two-Dimensional
MEDICAL -- Cardiology.
Biochemical markers.
Two-dimensional electrophoresis.
Proteomics -- methods.
Biological Markers -- analysis.
Electrophoresis, Gel, Two-Dimensional -- methods.
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization -- methods.
Appareil cardiovasculaire -- Maladies -- Aspect moléculaire.
Marqueurs biologiques.
Électrophorèse bidimensionnelle.
Cardiovascular system -- Diseases -- Molecular aspects.
Biochemical markers
Cardiovascular system -- Diseases -- Molecular aspects
Two-dimensional electrophoresis
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Vivanco, Fernando, 1948-
ISBN 1588295354