| Description |
1 online resource (xix, 682 pages) : illustrations |
| Series |
Methods in visualization |
|
Methods in visualization.
|
| Contents |
Slam-freezing, metal-mirror freezing / Daniele Spehner and Ludwig Edelmann -- Plunge-freezing (Holey carbon method) / Sacha De Carlo -- Controlled vitrification / Peter M. Frederik, Felix de Haas, and Marc M.H. Storms -- BAL-TEC HPM 010 high-pressure freezing machine / Andres Kaech -- High-pressure freezing Leica EMPACT / Dimitri Vanhecke and Daniel Studer -- Frozen-hydrated macromolecules for structural analysis / Corinne Crucifix, Gabor Papai and Patrick Schultz -- Two-dimensional crystals / Patrick Bron and Janet Vonck -- Cryo-negative staining / Sacha De Carlo -- Vitrification of dynamic microtubules / Isabelle Arnal, Marine Malleter, and Denis Chretien -- CEMOVIS : cryo-electron microscopy of vitreous sections / Jacques Dubochet [and others] -- Cryo-electron tomography / Andrew Leis, Manuela Gruska, and Wolfgang Baumeister -- Freeze-substitution / Bruno M. Humbel -- Cryo-fixation, freeze-substitution, rehydration and Tokuyasu cryo-sectioning / York-Dieter Stierhof [and others] -- Freeze-drying and embedding of biological material / Ludwig Edelmann -- The shadow of hydrated biological specimens / Daniel Thomas -- Cryo-fracture of self assembled systems in organic solvent / Marc Schmutz and Philippe J. Mesini -- Progressive lowering of temperature for immunolabeling and in situ hybridization / Annie Cavalier and Daniele Spehner -- Cryo-sectioning according to Tokuyasu / Bruno M. Humbel and York-Dieter Stierhof -- Cryo-preparation procedures for elemental imaging by SIMS and EFTEM / Christian Bordat and Jean-luc Guerquin-Kern -- Correlative light and electron microscopy / Heinz Schwarz and Bruno M. Humbel -- SDS-digested freeze-fracture replica labeling (SDS-FRL) / Yugo Fukazawa [and others] -- Immunolabeling of ultrathin sections with enlarged 1 nm gold or QDots / York-Dieter Stierhof -- 3-D electron tomography of cells and organelles / Willie J.C. Geerts, Bruno M. Humbel and Arie J. Verkleij -- Final considerations / Hellmuth Sitte |
| Summary |
While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable. The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations. Contributions to this handbook - Describe cryo-methods aimed at perfect preservation for fine structural analysis Teach how to arrest physiological processes by cryo-fixation Reveal the secrets for high-resolution snapshots of life by CEMOVIS Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding Highlight the |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Cryobiology -- Methodology
|
|
Electron microscopy -- Methodology
|
|
Cryopreservation -- methods
|
|
Microscopy, Electron -- methods
|
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SCIENCE / Life Sciences / Cytology
|
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SCIENCE / Research & Methodology
|
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SCIENCE / Microscopes & Microscopy
|
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Electron microscopy -- Methodology
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Cavalier, Annie.
|
|
Spehner, Danièle.
|
|
Humbel, Bruno M.
|
| ISBN |
9780849372278 |
|
0849372275 |
|
9781420006735 |
|
1420006738 |
|
9780367800833 |
|
0367800837 |
|
