| Description |
1 online resource (1 volume) : illustrations |
| Series |
Methods in molecular medicine ; 110 |
|
Methods in molecular medicine ; 110. 1543-1894
|
| Contents |
v. 1. An overview of chemosensitivity testing / R.D. Blumenthal -- Clonogenic cell survival assay / A. Munshi [and others] -- High-sensitivity cytotoxicity assays for nonadherent cells / M. Jules Mattes -- Sulforhodamine B assay and chemosensitivity / W. Voigt -- Use of the differential staining cytotoxicity assay to predict chemosensitivity / G.J. Kaspers -- Collagen gel droplet culture method to examine in vitro chemosensitivity / H. Kobayashi -- The MTT assay to evaluate chemosensitivity / J.D. Burton -- Histoculture drug response assay to monitor chemoresponse / S. Ohie [and others] -- In vitro testing of chemosensitivity in physiological hypoxia / R. Grigoryan [and others] -- Chemosensitivity testing using microplate adenosine triphosphate-based luminescence measurements / C.M. Kurbacher and I.A. Cree -- High-throughput technology: Green fluorescent protein to monitor cell death / M. Fortin [and others] -- DIMSCAN : A microcomputer fluorescence-based cytotoxicity assay for preclinical testing of combination chemotherapy / N. Keshelava [and others] -- The ChemoFx assay: An ex vivo cell culture assay for predicting anticancer drug responses / R.L. Ochs [and others] -- Evaluating response to antineoplastic drug combinations in tissue culture models / C.P. Reynolds and B.J. Maurer -- Image analysis using the fluorochromasia assay to quantify tumor drug sensitivity / J.F. Gibbs [and others] -- Immunohistochemical detection of ornithine decarboxylase as a measure of chemosensitivity testing / U. Bachrach -- Immunohistochemistry of p53, Bcl-2, and Ki-67 as predictors of chemosensitivity / M. Itaya [and others] |
| Summary |
Chemosensitivity testing is an ex vivo means of determining or enhancing the cytotoxic and/or cytostatic, or apoptosis-inducing effects of anticancer drugs. In Chemosensitivity, leading researchers and physicians working in academia and biotech companies describe state-of-the-art laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and nonadherent cells for single agents and combinations of agents. In addition to immunohistochemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The authors also provide guidance on how best to analyze the data derived from the protocols. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients. Cutting-edge and highly practical, the two volumes of Chemosensitivity provide a comprehensive collection of readily reproducible techniques for the in vitro and in vivo screening of new agents and a set of proven approaches to understand mechanistically why certain cancer cell lines (in vitro) of tumors (in vivo) are more, or less, sensitive to a particular agent |
| Analysis |
neoplasms |
|
geneesmiddelenbehandeling |
|
drug therapy |
|
geneesmiddelen |
|
drugs |
|
geneeskunde |
|
medicine |
|
humane ziekten |
|
human diseases |
|
in vivo experimenten |
|
in vivo experimentation |
|
Medicine (General) |
|
Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Cancer -- Chemotherapy -- Laboratory manuals
|
|
Antineoplastic agents -- Effectiveness -- Laboratory manuals
|
|
Cancer cells -- Laboratory manuals
|
|
Cancer -- Molecular aspects -- Laboratory manuals
|
|
Drug resistance in cancer cells.
|
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Antineoplastic Agents -- pharmacology
|
|
Drug Screening Assays, Antitumor -- methods
|
|
Drug Resistance, Neoplasm
|
|
Models, Animal
|
|
Neoplasms -- drug therapy
|
|
MEDICAL -- Oncology.
|
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HEALTH & FITNESS -- Diseases -- Cancer.
|
|
Drug resistance in cancer cells
|
|
Cancer cells
|
|
Cancer -- Chemotherapy
|
|
Cancer -- Molecular aspects
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Blumenthal, Rosalyn D.
|
| ISBN |
1592598692 |
|
9781592598694 |
|
