| Description |
1 online resource |
| Contents |
Title page; Table of Contents; Copyright; Contributors; Preface; Chapter 1: Food industry current status; Abstract; Introduction; Molecular methods currently used; Laboratory automation in molecular methods; Challenges; Acceptance of use by the food industry; Compliance with current legal requirements; Disclaimer; Chapter 2: Future directions for molecular microbial diagnostic methods for the food industry; Abstract; Introduction; Evolution of food microbiology diagnostics: from petri dishes to PCR; Why introduce an alternative molecular diagnostic method? |
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Characteristics of an ideal food molecular methodPast and current challenges; Current challenges; Concluding remarks; Chapter 3: Current status of molecular microbiological techniques for the analysis of drinking water; Abstract; Introduction and overview; The current state of play; The influence of standard and reference methods; Molecular techniques for testing potable water quality; Application of molecular techniques to wastewater; Conclusions; Chapter 4: What is now required for water?; Abstract; Introduction and overview; Indicator organisms; Pathogens in drinking water |
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Total heterotrophic bacteriaFeasibility of the use of routine rapid water molecular methods; Future predictions; Conclusions; Chapter 5: CEN/ISO standards for both culture and molecular methods; Abstract; Introduction; Standards, standardization bodies, and structures in the microbiology of the food chain; Standards developed in the microbiology of the food chain; Status of novel technologies; Conclusions; Chapter 6: Laboratory validation, verification, and accreditation of molecular methods; Abstract; Alternative methods; Foundations of an accredited laboratory; Quality systems |
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Cornerstones of accreditationMaintaining accreditation; Customer education; Health and safety; Chapter 7: DNA extraction: finding the most suitable method; Abstract; Boiling method; Column extraction; Magnetic beads; FTA™ cards; RNA extraction methods; Conclusions; Chapter 8: Assessing organism viability and interpreting genomic unit versus colony forming unit data for water and food borne microorganisms, such as Legionella, Campylobacter, Salmonella, and Listeria; Abstract; Introduction; Polymerase chain reaction; Assessing microbial viability; Overcoming the viability hurdle |
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When to use viability discriminationStandardization and quality assurance; Proficiency testing; Conclusions; Chapter 9: MALDI-TOF MS: a rapid microbiological confirmation technique for food and water analysis; Abstract; Introduction and overview; Current confirmation methods and advantages of MALDI-TOF MS; MALDI-TOF MS Listeria speciation validation; Chapter 10: Chapter highlights, future requirements, and conclusions; Chapter highlights; Future requirements and conclusions; Index |
| Summary |
Molecular Microbial Diagnostic Methods: Pathways to Implementation in the Food and Water Industries attempts to address the shortage of guidance on implementation of molecular-based methods for routine diagnostic laboratories. What industry and analysts can expect from routine use of these methods is discussed and outlined. The book uncovers industry needs for the use of molecular methods by providing a brief history of water and food analysis for the pathogens of concern. It also describes the potential impact of current and cutting-edge molecular methods. It discusses the advantages of the implementation of molecular methods, describes information on when and how to use specific methods, and presents why one should utilize them for pathogen detection in the routine laboratory. The reference material in the book is also pertinent for anyone carrying out microbiological analysis at the research level and covers a wide spectrum of classical and cutting-edge methods |
| Notes |
Includes index |
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Online resource; title from PDF title page (ScienceDirect, viewed October 15, 2015) |
| Subject |
Diagnostic microbiology -- Methodology
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Molecular microbiology -- Methodology
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SCIENCE -- Life Sciences -- Biology.
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SCIENCE -- Life Sciences -- Microbiology.
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Food -- Microbiology.
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Water -- Microbiology.
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| Genre/Form |
Laboratory manuals.
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Cook, Nigel, editor.
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D'Agostino, Martin, editor
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Thompson, K. C. (Kenneth Clive), 1944- editor.
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| ISBN |
9780124171701 |
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0124171702 |
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