| Description |
1 online resource (1 volume) : illustrations |
| Series |
Methods in molecular medicine ; 95 |
|
Methods in molecular medicine ; 95.
|
| Contents |
Overview of commercial HBV assays systems / S. Zeuzem-Detection of HBV DNA in serum using a PCR-based assay / H.T. Chung-Detection of HBV DNA by oligonucleotide probing / H.J. Lin-Detection of HBV RNA in serum of patients / W. Zhang [and others]-Quantification of HBV covalently closed circular DNA from liver tissue by real-time PCR / S. Bowden [and others]-In situ hybridization for the detection and localization of HBV DNA in liver sections / V.C. Lai and J.Y. Lau-Quantitative assay of hepatitis b surface antigen in serum or plasma using laurell electrophoresis / W.H. Gerlich, U. Wend and D. Glebe-In situ detection of hepatitis b viral antigens / J.W. Fang and J.Y. Lau-Detection of hepatitis b virus x antigen by immunohistochemistry and western blotting / J. Liu and M.A. Feitelson-Detection of serum hdv RNA by rt-PCR / A. Smedile, M.G. Niro and M. Rizzetto-Nonradioisotopic in situ hybridization for hdv RNA / F. Negro-Detection and characterization of small and large hdv antigens / T.B. Macnaughton and M.M. Lai-Immunohistochemical detection of hepatitis delta antigen / K. Abid and F. Negro-HBV vaccine-escape variants / A.A. Basuni and W.F. Carman-HBV genotyping and analysis for unique mutations / A. Ayres, S. Locarnini and A. Bartholomeusz-A one-filter-three-probe assay for defective interference (di) effects of naturally occurring core inteRNAl deletion (cid) variants of human hepatitis b virus / C. Shih and T.T. Yuan-Detection of hypermodified middle-envelope (m) proteins secreted from naturally occurring HBV variants containing a pres2 inteRNAl deletion / C. Shih and P.C. Tai-Hepatitis b viral genotyping with the research inno-lipa HBV genotyping line probe assay / S. De Gendt [and others]-Processing of hepatitis b virus surface proteins / V. Bruss-Binding of duck carboxypeptidase d to duck hepatitis b virus / S. Urban-Nucleoprotein transport of HBV capsid particles / M. Kann-Phosphorylation analysis of hepatitis b virus core protein in mammalian cells / J. Li [and others]-Study of HBV replication capacity in relation to sequence variation in the precore and core promoter regions / F. Zoulim, S. Tong and C. Trepo-A cis/trans genetic test for pleiotropic phenotypes associated with a frequent naturally occurring mutation at amino acid 97 of HBV core protein / C. Shih and T.T. Yuan-Studying dHBV polymerase by in vitro transcription and translation / J. Hu-Expression and purification of functional hepatitis b virus polymerase in the baculovirus insect cell system / L. Lott, L. Notvall and R.E. Lanford-Localization of duck hepatitis b virus polymerase within cells / E. Yao and J.E. Tavis-Endogenous polymerase assay for the analysis of hepatitis b virus in transgenic mice / Z. Xu and J.H. Ou-Transcriptional control of hepatitis b virus / A.K. Raney-In vitro reconstitution of epsilon-dependent duck hepatitis b virus replication initiation / J. Beck and M. Nassal-Hepatitis b viruses : A triple threat for malignant transformation of hepatocytes / C.E. Rogler |
| Summary |
Despite the availability of an effective vaccine, there are still 400 million people worldwide who are chronically infected with the hepatitis B and D viruses (HBV and HDV), and more work still needs to be done in all of the key areas of managing the disease. In Hepatitis B and D Protocols, leading investigators and clinicians have joined forces to create a broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this first of two volumes Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Taken together, Hepatitis B and D Protocols, Volume 1: Detection, Genotypes, and Characterization and Volume 2: Immunology, Model Systems, and Clinical Studies offer both new and experienced investigators an encyclopedic collection of powerful tools for studying HBV and HDV infections, as well as an essential resource for finding new therapies to treat chronically infected patients |
| Analysis |
hepatitis |
|
hepatitis b |
|
hepatitis b virus |
|
hepatitis d |
|
hepatitis delta virus |
|
detectie |
|
detection |
|
virusziekten |
|
viral diseases |
|
virale hepatitis |
|
viral hepatitis |
|
biologische technieken |
|
biological techniques |
|
rna viruses |
|
moleculaire detectie |
|
molecular detection |
|
moleculaire merkers |
|
molecular markers |
|
protocollen |
|
protocols |
|
Virology |
|
Virologie |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| In |
Springer Protocols |
| Subject |
Hepatitis B -- Laboratory manuals
|
|
Hepatitis B virus -- Laboratory manuals
|
|
Delta-associated agent -- Laboratory manuals
|
|
Delta infection -- Laboratory manuals
|
|
Hepatitis B.
|
|
Delta infection.
|
|
Hepatitis B
|
|
Hepatitis D
|
|
MEDICAL -- Hepatology.
|
|
Delta-associated agent
|
|
Delta infection
|
|
Hepatitis B
|
|
Hepatitis B virus
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Hamatake, Robert Kiyoshi, 1953-
|
|
Lau, Johnson Y. N. (Johnson Yiu-Nam)
|
| ISBN |
9781592596690 |
|
159259669X |
|
9781588291059 |
|
1588291057 |
|
