pt. I. Introduction -- 1. Hello BLAST -- pt. II. Theory -- 2. Biological Sequences -- 3. Sequence Alignment -- 4. Sequence Similarity -- pt. III. Practice -- 5. BLAST -- 6. Anatomy of a BLAST Report -- 7. A BLAST Statistics Tutorial -- 8. 20 Tips to Improve Your BLAST Searches -- 9. BLAST Protocols -- pt. IV. Industrial-Strength BLAST -- 10. Installation and Command-Line Tutorial -- 11. BLAST Databases -- 12. Hardware and Software Optimizations -- pt. V. BLAST Reference -- 13. NCBI-BLAST Reference -- 14. WU-BLAST Reference -- pt. VI. Appendixes -- A. NCBI Display Formats -- B. Nucleotide Scoring Schemes -- C. NCBI-BLAST Scoring Schemes -- D. blast-imager.pl -- E. blast2table.pl
Summary
Sequence similarity is a powerful tool for discovering biological function. Just as the ancient Greeks used comparative anatomy to understand the human body and linguists used the Rosetta stone to decipher Egyptian hieroglyphs, today we can use comparative sequence analysis to understand genomes. BLAST (Basic Local Alignment Search Tool), is a sophisticated software package for rapid searching of nucleotide and protein databases. It is one of the most important software packages used in sequence analysis and bioinformatics. Most users of BLAST, however, seldom move beyond the program's defaul
Notes
"An essential guide to the Basic Local Alignment Search Tool"--Cover
