| Description |
1 online resource (x, 295 pages) : illustrations (some color) |
| Series |
Methods in Molecular Biology, 1064-3745 ; 1300 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1300. 1064-3745
|
| Contents |
A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells -- Microscopy Techniques to Examine DNA Replication in Fission Yeast -- High-Resolution Analysis of Mammalian DNA Replication Units -- Analysing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing -- Measuring DNA Content by Flow Cytometry in Fission Yeast -- Incorporation of Thymidine Analogs for Studying Replication Kinetics In Fission Yeast -- Edu Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast -- Determination Of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection -- Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo -- Detection And Sequencing Of Okazaki Fragments In S. Cerevisiae -- Chip-Seq To Analyze The Binding Of Replication Proteins To Chromatin -- Chromatin Immunoprecipitation To Detect DNA Replication And Repair Factors -- Molecular Genetic Methods To Study DNA Replication Protein Function In Haloferax Volcanii, A Model Archaeal Organism -- Single-Molecule Observation Of Prokaryotic DNA Replication -- Analysing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast -- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis -- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes |
| Summary |
Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories |
| Analysis |
biomedische wetenschappen |
|
biomedicine |
|
moleculaire genetica |
|
molecular genetics |
|
Medicine (General) |
|
Geneeskunde (algemeen) |
| Notes |
Includes index |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed May 8, 2015) |
| Subject |
DNA replication -- Laboratory manuals
|
|
DNA replication.
|
|
DNA Replication
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DNA replication
|
| Genre/Form |
Laboratory manuals
|
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Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Vengrova, Sonya, editor.
|
|
Dalgaard, Jacob Z., editor.
|
| ISBN |
9781493925964 |
|
1493925962 |
|
1493925954 |
|
9781493925957 |
|
