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Title Apoptosis methods and protocols / edited by Hugh J.M. Brady
Published Totowa, N.J. : Humana Press, ©2004

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Description 1 online resource (xi, 304 pages) : illustrations
Series Methods in molecular biology, 1064-3745 ; 282
Methods in molecular biology (Clifton, N.J.) ; v. 282
Contents Measurement of Apoptosis by DNA Fragmentation / Demetrius Matassov, Terri Kagan, Julie Leblanc, Marianna Sikorska, and Zahra Zakeri -- Measurement of Caspase Activity in Cells Undergoing Apoptosis / Sharad Kumar -- Flow Cytometry-Based Methods for Apoptosis Detection in Lymphoid Cells / Owen Williams -- Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays / Richard C. Duke -- Detecting and Quantifying Apoptosis in Tissue Sections / Vicki Save, Peter A. Hall, and Philip J. Coates -- Immunoassay for Single-Stranded DNA in Apoptotic Cells / Oskar S. Frankfurt -- Methods to Measure Membrane Potential and Permeability Transition in Mitochondria During Apoptosis / Naoufal Zamzami and Guido Kroemer -- Measurement of Changes in Intracellular Calcium During Apoptosis / David J. McConkey and Leta Nutt -- Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2 / Gabriel Gil-Gómez -- Assays to Measure Stress-Activated MAP Kinase Activity / Carme Caelles and Mónica Morales -- Methods for Culturing Primary Sympathetic Neurons and for Determining Neuronal Viability / Jonathan Whitfield, Stephen J. Neame, and Jonathan Ham -- Immunocytochemical Techniques for Studying Apoptosis in Primary Sympathetic Neurons / Stephen J. Neame, Jonathan Whitfield, and Jonathan Ham -- Measuring Programmed Cell Death in Plants / Ludmila Rizhsky, Vladimir Shulaev, and Ron Mittler -- Detection of Apoptosis in Drosophila / Kimberly McCall and Jeanne S. Peterson -- Measurement of Apoptotic Cell Clearance In Vitro / Andrew Devitt and Christopher D. Gregory -- Yeast Two-Hybrid Screening for Proteins Interacting With the Anti-Apoptotic Protein A20 / Karen Heyninck, Sofie Van Huffel, Marja Kreike, and Rudi Beyaert -- The Yeast Three-Hybrid System As a Tool to Study Caspases / Wim van Criekinge, Peter Schotte, Karen Heyninck, and Rudi Beyaert -- Cloning of Apoptosis-Related Genes by Representational Difference Analysis of cDNA Michael Hubank -- Identification of Apoptosis Regulatory Genes Using Insertional Mutagenesis / Joëlle Thomas, Yann Leverrier, Anne-Laure Mathieu, and Jacqueline Marvel
Summary The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research in the 21st century. In Apoptosis Methods and Protocols, highly experienced researchers in the field of apoptosis describe in step-by-step detail the techniques they have perfected to investigate the critical steps involved in the apoptotic process. These readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Apoptosis Methods and Protocols constitutes a key technical reference to the significant methodologies used in the field, as well as offering novice and experienced researchers alike powerful tools to illuminate the phenomenon of programmed cell death
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Apoptosis -- Laboratory manuals
Molecular biology -- Laboratory manuals
DNA Damage -- physiology
Apoptosis -- physiology
SCIENCE -- Life Sciences -- Anatomy & Physiology.
Apoptosis
Molecular biology
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Brady, Hugh J. M
LC no. 2003027914
ISBN 0896038734
9780896038738
1592598129
9781592598120