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Title Cytoskeleton dynamics : methods and protocols / edited by Helder Maiato
Published New York, NY : Humana Press, 2020


Description 1 online resource (xi, 390 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; 2101
Methods in molecular biology (Clifton, N.J.) ; v. 2101. 1064-3745
Summary This book provides a comprehensive overview of the state-of-the-art methodology to investigate how actin (and actinlike) microfilaments and microtubules work to accomplish different cellular functions in different cellular contexts from bacteria to humans. The book covers extensive and exciting methodologies for protein purification and in vitro reconstitution of several cytoskeleton properties, as well as the functional dissection of microtubule- and actin-based structures, such as centrioles, mitotic spindles, axons, and cytokinetic contractile rings. It also provides a methodology for the study and manipulation of microtubules and actin, including the study of their nucleation, ultrastructural and/or dynamic properties (e.g., using optogenetics), and the functional dissection of post-translational modifications. There is a focus on specific biological processes, inevitably on mitosis, but also on bacterial division, meiosis, cytokinesis, axonal transport, and nuclear migration
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed January 15, 2020)
Subject Cytoskeleton -- Laboratory manuals
Cell physiology.
Cytoskeleton -- physiology
Cytoskeleton -- metabolism
Cell Physiological Phenomena
Cytological Techniques -- methods
Cell physiology
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Maiato, Helder, editor
ISBN 9781071602195