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Title Cytomegalovirus protocols / edited by John Sinclair
Published Totowa, N.J. : Humana Press, ©2000

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Description 1 online resource (x, 182 pages) : illustrations
Series Methods in molecular medicine ; 33
Methods in molecular medicine ; 33.
Contents Immunological Methods for the Detection of Human Cytomegalovirus / Andrew D. Yurochko, Eng-Shang Huang -- Identification of Immunoreactive Viral Proteins / Walter Hinderer, Bodo Plachter, Rolf Vornhagen -- Analysis of Human Cytomegalovirus Using the Polymerase Chain Reaction / Marc Mendelson -- Analysis of Cytomegalovirus Ligands, Receptors, and the Entry Pathway / Teresa Compton -- Analysis of Cytomegalovirus Gene Expression by Transfection / Anamaris M. Colberg-Poley, Vera E. Soltero, Richard-Fabian Schumacher -- Analysis of Protein-Protein Interactions During Cytomegalovirus Infection / Richard C. Caswell -- Analysis of Protein-DNA Interactions During Cytomegalovirus Infection / Stefan Floss, Thomas Stamminger -- Analysis of Cytotoxic T-Cell Responses to Human Cytomegalovirus Infection / Mark R. Wills, J.G. Patrick Sissons -- Methods in Anti-HCMV Research / Johan Neyts, Graciela Andrei, Robert Snoeck, Astrid Meerbach, Erik De Clercq -- Construction of Recombinant Human Cytomegalovirus / Richard F. Greaves
Summary In Cytomegalovirus Protocols (CMV), John Sinclair and a panel of expert investigators present a comprehensive collection of cellular and molecular techniques for the analysis of cytomegalovirus biology and its pathogenetic mechanisms. The methods-all described in step-by-step detail with ready reproducibility in mind-range from basic virus culture to complex molecular analysis of CMV structure and function. Included are methods for CMV detection using both immunological and biological techniques, methods for analyzing fundamental aspects of the CMV infection cycle, and methods for analyzing T cell response to cytomegalovirus infection in the human host. Comprehensive and state-of-the-art, Cytomegalovirus Protocols provides investigators with a powerful collection of the key methods that are illuminating not only the basic biology of this complex and intriguing human herpesvirus, but also its significant role in the pathogenesis of human infectious diseases as well as their emergent therapies
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
Print version record
In Springer Protocols
Subject Cytomegalovirus infections -- Laboratory manuals
Cytomegaloviruses -- Laboratory manuals
Genetics -- Technique.
Cytomegalovirus -- isolation & purification
Cytomegalovirus -- genetics
Cytomegalovirus Infections -- genetics
Genetic Techniques
MEDICAL -- Microbiology.
Cytomegalovirus infections
Cytomegaloviruses
Wetenschappelijke technieken.
Genre/Form Handbooks and manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Sinclair, John, Ph. D.
ISBN 9781592592449
1592592449
0585235678
9780585235677
0896037495
9780896037496