| Description |
1 online resource (xv, 444 pages) : color illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; 464, pt. 2 |
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Methods in molecular biology (Clifton, N.J.) ; volume 464, pt. 2
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| Contents |
The intranuclear environment / S. Schnell and R. Hancock-Purification of nuclei and preparation of nuclear envelopes from skeletal muscle / G.S. Wilkie and E.C. Schirmer-Isolation of highly purified yeast nuclei for nuclease mapping of chromatin structure / J.C. Reese, H. Zhang and Z. Zhang-Working with oocyte nuclei: Cytological preparations of active chromatin and nuclear bodies from amphibian germinal vesicles / G.T. Morgan-Preparation of arabidopsis nuclei and nucleoli / P. McKeown, A.F. Pendle and P.J. Shaw-High-yield isolation and subcellular proteomic characterization of nuclear and subnuclear structures from trypanosomes / J.A. DeGrasse [and others]-Methods for studying the nuclei and chromosomes of dinoflagellates / M.O. Soyer-Gobillard-Isolation of nucleoli from ehrlich ascites tumor cells and dynamics of nascent rna within isolated nucleoli / M. Thiry and D. Ploton-Time-lapse microscopy and fluorescence resonance energy transfer to analyze the dynamics and interactions of nucleolar proteins in living cells / E. Louvet [and others]-Three-dimensional reconstruction of nucleolar components by electron microscope tomography / P. Tchelidze [and others]-The perinucleolar compartment (pnc): Detection by immunohistochemistry / A. Slusarczyk and S. Huang-Isolation of the constitutive heterochromatin from mouse liver nuclei / O.V. Zatsepina, O.O. Zharskaya and A.N. Prusov-Isolation of pathology-associated intranuclear inclusions / C. Iwahashi and P.J. Hagerman-The nuclear ubiquitin-proteasome system: Visualization of proteasomes, protein aggregates, and proteolysis in the cell nucleus / A. von Mikecz [and others]-Multicolor 3d fluorescence in situ hybridization for imaging interphase chromosomes / M. Cremer [and others]-Fluorescent transgenes to study interphase chromosomes in living plants / A.J. Matzke [and others]-Analysis of telomeres and telomerase / J. Fajkus, M. Dvorackova and E. Sykorova-Combined immunofluorescence, rna fluorescent in situ hybridization, and DNA fluorescent in situ hybridization to study chromatin changes, transcriptional activity, nuclear organization, and x-chromosome inactivation / J. Chaumeil [and others]-Analysis of the mobility of DNA double-strand break-containing chromosome domains in living mammalian cells / P.M. Krawczyk [and others] |
| Summary |
Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development of the new, powerful biochemical and biophysical methodologies described here are essential in this quest. In The Nucleus, researchers from more than forty leading international laboratories describe state-of-the-art methods for isolating nuclei and their components and for studying their structure and activities, including some pathology-associated features. Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging presents biophysical approaches to study the mechanical properties of nuclei, together with a comprehensive range of imaging methods. These include FISH, FRAP, FRET, molecular beacons, fluorescence correlation spectroscopy, single molecule tracking, and combing DNA for optical microscopy, recognition imaging for atomic force microscopy, and hybridisation, tomography, and spectroscopic imaging for electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters contain lists of necessary materials and reagents, readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls. The Nucleus, Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging provides a comprehensive collection of the cutting-edge methods making a major contribution to understanding the nucleus and its nanostructure today |
| Analysis |
biochemie |
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biochemistry |
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biologische technieken |
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biological techniques |
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celbiologie |
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cellular biology |
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afbeelden |
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imagery |
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levenswetenschappen |
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life sciences |
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radiologie |
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radiology |
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Biology (General) |
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Biologie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Cell nuclei -- Research -- Laboratory manuals
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Cytology -- Technique.
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Cell Nucleus -- genetics
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Cell Nucleus -- physiology
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Cytological Techniques
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Cell Nucleus -- physiology -- Laboratory Manuals.
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Cytological Techniques -- Laboratory Manuals.
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Noyau cellulaire.
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Transcription génétique.
|
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Cell nuclei -- Research.
|
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Cell Nucleus -- genetics -- Laboratory Manuals.
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Cytology -- Technique
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Noyau cellulaire.
|
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Transcription génétique.
|
| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Hancock, Ronald.
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| ISBN |
9781603274616 |
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1603274618 |
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9781603274609 |
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160327460X |
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