Title Cells and culture : Proceedings of the 20th ESACT Meeting, Dresden, Germany, June 17-20, 2007 / edited by Thomas Noll

Published Dordrecht ; London : Springer, 2009

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Description 1 online resource Series ESACT proceedings ; v. 4 ESACT proceedings ; v. 4. Contents Machine generated contents note: pt. I Single-Cell Analysis and Engineering -- Targeted Gene Knockdown Effects on Recombinant Protein Secretion in CHO Cells / Kevin J. Kayser -- RNA Silencing Suppressors Boost the Production of Recombinant Proteins and Viruses / Eddy Arnoldussen -- Towards the Use of CHO Produced Recombinant Extracellular Matrix Proteins as Bioactive Elements in a 3-D Scaffold for Tissue Engineering / F.M. Wurm -- Transient Gene Expression in Chinese Hamster Ovary Cells at Low Temperature -- The Effects of Cold-Induced Proteins and an mRNA Regulatory Element / Florian M. Wurm -- Single-Cell Approach in Influenza Vaccine Production: Apoptosis and Virus Protein Production / Udo Reichl -- Chondrogenic Differentiation of Human Mesenchymal Stem Cells During Multiple Subcultivation / Peter Czermak -- Cell Xpress[™]-Assisted Analysis of Clone Stability in Recombinant Chinese Hamster Ovary Cells / Matthew V. Caple -- Evaluation of the Intrinsic IgG Production Capabilities of Different Chinese Hamster Ovary Parental Cell Lines / Kevin J. Kayser -- Cell Xpress[™] Technology Facilitates High-Producing Chinese Hamster Ovary Cell Line Generation Using Glutamine Synthetase Gene Expression System / Kevin J. Kayser -- CHO-DG44 Cell Line Development by FLP-Targeting -- High Level Glycoprotein Expression with Significantly Decreased Time Lines / Volker Sandig -- Transgene Copy Number -- Impact on Clone Performance / Volker Sandig -- Single Use Bioreactors: Expressing Protein in Mammalian Cell Suspension / Kevin A. Auton -- pt. II Applied Integrative Physiology -- Gene Modified Hematopoietic Stem Cells for the Treatment of Primary Immunodeficiency Diseases / R. Seger -- Avian Cell Line for Production of Highly Attenuated Viral Vectors / Volker Sandig -- Microelectronic Cellular Vitality Monitoring / B. Wolf Note continued: Evaluation of Alternative Signal Sequences / R. Young -- Process Development for the GMP Production of N-Acetylgalactosamine-6-Sulfate Sulfatase (GALNS) Expressed by CHO Cells / Christoph Heinzen -- Improvement of a CHO Fed-Batch Process by Fortifying with Plant Peptones / Veronique Chotteau -- O-Glycans on Recombinant MUCI Produced in CHO K1 Cells Become Less Sialylated with Increased Protein Productivity, as Determined by LC-ESI MS / Gunnar C. Hansson -- Multiple Minibioreactor Platform for Parallel and Automated Mammalian Cell Culture / F. Godia -- Cultivation of Adherent-Dependent Animal Cells on Microcarriers in a New Disposable Reactor / J. Castillo -- CHO Cells Cultivation and Antibodies Production in a New Disposable Bioreactor Based on Magnetic Driven Centrifugal Pump / J. Castillo -- Stability and Productivity of CHO Pools with Respect to Culture Age, Cryopreservation and 20 L Bioreactor Cultivation / Mats Lundgren -- In Vitro Disassembly and Reassembly of Triple-Layered Rotavirus-Like Particles / Paula M. Alves -- Influence of Culture Conditions on Insect Cell Growth and Protein Production-Comparison of Wave Bioreactor and Shake Flasks / Petra Helfrich -- Process Intensification Based on Nano-Structured Carbon Carrier Materials and Disposable Devices / Soheil Asgari -- Accelerating Fed-Batch Process Development Using Rationally Designed Feed Media / Tom Fletcher -- Development of a Robust Small-Scale Production Format that Is Predictive of Bioreactor Performance / Martin Sinacore -- Biomass Monitoring and CHO Cell Culture Optimization Using Capacitance Spectroscopy / Leda R. Castilho -- Characterizing Physiology and Metabolism of High-Density CHO Cell Perfusion Cultures Using 2D-NMR Spectroscopy / Konstantin Konstantinov Note continued: BI HEX[™] -- Platform for Fast Track Generation of High Producer Cell Lines Leading to High-Titer Processes for Production of Therapeutic Proteins from Mammalian Cells / Stefanos Grammatikos -- Management of Handling, Long-Term Stability and Shipping of Human T-Lymphocytes Bags for Clinical Studies / M.L. Nolli -- Mass Transfer in the CELL-tainer® Disposable Bioreactor / Pieter van der Heiden -- Efficient Production of Human Monoclonal Antibodies by Combining In Vitro Immunization and Phage Display Methods / Sanetaka Shirahata -- Measurement and Control of Viable Cell Density in a Mammalian Cell Bioprocessing Facility: Validation of the Software / J.P. Carvell -- Development, Validation, and Application of a Fully Integrated Automation System for Screening and Selection of High Yielding Production Cell Lines / John Mott -- Monitoring of Cell Activity: Online Oxygen Uptake Rates in Pulsed Aerated Cell Culture / U. Reichl -- pt. IV Systems Biotechnology -- Metabolite Analysis in Mammalian Cells: How to Generate Reliable Data Sets? / Udo Reichl -- Cell-to-Cell Communication for Cell Density-Controlled Bioprocesses / Martin Fussenegger -- Metabolome Analysis in Mammalian Cells: Development and Application of a Sampling Technique / M. Oldiges -- Differential Expression Profiling of Industrially Relevant CHO Cell Phenotypes Using a Proprietary CHO-Specific Microarray and Proteomics Technology Platforms / Tim Charlebois -- Towards a Systems-Level Understanding of Increased Specific Productivity in Proliferation Arrested Myeloma NSO Cells / Mohamed Al-Rubeai -- Proteomic Analysis of Influenza a Virus Infected Mammalian Cells by 2D-DIGE / U. Reichl -- Involvement of SRC -- and MAP Kinase-Signalings in the Effect of Sericin on Cellular Proliferation and Survival / Satoshi Terada Note continued: Unfolded Protein Response and Recombinant Protein Production from in Vitro Cultured Mammalian Cells / Mark Smales -- Scalable Process for Helper-Dependent Adenoviral Vector Production Using PEI-Derived Transfection Strategy in Suspension Culture / A. Kamen -- Functional Analysis of ER Stress Pathway Genes for Apoptosis of NS/0 Cell Line Using RNAi Methods / Kripa Ram -- Identification of New Protein Substrate Candidates of Transglutaminase in Rat Liver Extracts: Use of 5-(Biotinamido) Pentylamine as a Probe / Koji Ikura -- Metabolic Flux Distributions of Adherently Growing MDCK Cells in Different Media / U. Reichl -- Expression of Dermal Extracellular Matrix Proteins in a Newly Developed Full-Thickness Skin Model / Klaus R. Schroder -- Development of Preparations with Antiviral and Immunostimutory Effects from Extracts of Green Parts of Coniferous Plants / A. Vilesov -- Serum-Free Transfection of CHO Cells with Chemically Defined Transfection System to Generate Transient and Stable Cell Lines / Kunert Renate -- "Glycomics": Development and Characterization of Glycan-Based Biotechnological Products / Markus Berger -- Development of a Cell-Culture-Based Platform for Viral Vaccine Production / M. Etcheverrigaray -- Screening of Natural Compounds Affecting Type I Interferon Signalling / W. Muller -- pt. V Competing and Complementary Approaches to Animal Cell Technologies -- Therapeutic Proteins from Transgenic Cows' Milk / M.E. Criscuolo -- Development of Edible Plant Vaccines / Inge Broer -- Human Cell Lines for Production of Biopharmaceuticals / Gero Waschutza -- Concentration and Purification of Densonucleosis Virus by Tangential Membrane Filtration and by Ion Exchange Membranes / S. Ranil Wickramasinghe -- Effect of ManNAc and its Derivatives on Glycosylation to Proteins Produced by Mammalian Cell Culture / Satoshi Terada Note continued: Comparing Vero and MDCK Cells for Influenza A Virus Production in Microcarrier Systems / U. Reichl -- Expansion of Human Articular Chondrocytes on Microcarriers Enhances the Production of Cartilage Specific Matrix Components / Ralf Portner -- Automated Cell Culture Handling -- Characteristics and Procedures / Ursula Graf-Hausner -- Bioreactor Satellite Culture Experiments in the Start-Up of a Cell Culture Technical Support Lab / Patrick Salou -- Advantages of Hydrodynamic Cell Separation in Industrial Cell Culture Processes / Roland Wagner -- Optimization and Characterization of the Process for Large Volume Cell Banking in Bags / Chun Zhang -- Development of Screening Method for IgA-Promoting Factors Derived from Food Extracts Using a Human Myeloma Cell Line / Hiroharu Kawahara -- Cryopreservative Solution Using Sericin / Satoshi Terada -- Fructan as a Novel Effective Factor for Mammalian Cell Culture / Satoshi Terada -- Online Determination of Oxygen Uptake and Carbon Dioxide Production Rates in Mammalian Cell Culture Using Mass Spectrometry / T.W. Schulz -- Optimisation of mAb Concentration in Microcarrier Based Perfusion Compared to Batch/Fed-Batch Cultivation / Gerald Bluml -- Characterization of the Novel Human AGE1hn Cell Line for Production of Recombinant Proteins / T. Noll -- Rapid Selection of Optimal Formulations for Divergent Clones Through Screening Chinese Hamster Ovary Media Library / Matthew V. Caple -- Development of a Vaccine Candidate Against Heartwater / P.M. Alves -- Animal Component Free T-Cell Culture / J.H. Manwaring -- Characterization of Cholesterol-Independent GS-NS/0 Recombinant Antibody Cell Lines / Kripa Ram -- Development of a Fed-Batch Process for the Production of a Recombinant Protein X in CHO-GS System -- Case Study from the Cell to Reactor Process Ready for Pilot Scale Cultivation / Michael Oberg Note continued: Scaffolds for Articular Joint Tissue Engineering / F. Godia -- Effect of Tunisian Aromatic Plant Extracts on Melanogenesis / Hiroko Isoda -- Effects of Capsaicin on Energy Metabolism by Human Intestinal Epithelial Cell Line Caco-2 / Hiroko Isoda -- Human A1AT Production -- Propagating a Newly Developed Human Cell Line in a Novel Disposable Perfusion Bioreactor / Uwe Marx -- Characterization of Diffusion and Flow Relations in the Novel Membrane Based Perfusion Bioreactor / Uwe Marx -- Protective Effect of Di-O-Caffeoylquinic Acid on Human-Derived Neurotypic SH-SY5Y Cells against Alzheimer's Disease Amyloid-Beta-Induced Toxicity / Hideyuki Shigemori -- Flow Through Ceramic Foams -- A Future Cell Culture Challenge / R. King -- Measles Virus Production in MRC-5 Cells Grown on Microcarriers in a Stirred Bioreactor / Hela Kallel -- New System for the Enrichment of Cell Subclones Secreting High Levels of IgG Using Magnetic Cell Sorting (MACS® Technology) / Ulf Bethke -- Soy Hydrolysate Optimization for Cell Culture Applications / Matthew V. Caple -- Effect of Bioreactor pH and Temperature on Protein Glycosylation in Perfusion Cultures of Mammalian Cells / Chun Zhang -- Evaluation of Cell Growth Characteristics on Chitosan-Alginate Membranes to Assess Their Potential Application on Highly Exuding Skin Lesions and In Vivo Evaluation in Wounded Cat / Angela Maria Moraes -- Vaccine Production Utilizing the Potential of Microcarriers in Disposable Bioreactor / G. Blueml -- Characterization of an Anti-Idiotypic Antibody Blocking the Capacity of the HIV-1 Specific nMAb 2F5 / Renate Kunert -- Cultivation of PER. C6® Cells in the Novel CELL-Tainer[™] High-Performance Disposable Bioreactor / N. Oosterhuis -- Scale-Up of a PER.C6® Fed-Batch Process in 50 and 250 L Hyclone Single Use Bioreactors Compared to 50 and 250 L Stainless Steel Bioreactors / G. M. Zijlstra Note continued: Capacitance Sensor as a Robust Tool for Cell Culture Monitoring in Process Development and Manufacturing / Gianni Baer -- Effect of Different Cell Culture Medium Surfactants on Cell Growth and Viability / Elisabeth Lindner -- Conventional Stirred Bioreactor Control System for Monitoring and Controlling pH and DO in a Wave Bioreactor / Elisabeth Lindner -- On-Line Monitoring of Vero Cells Cultures During the Growth and Rabies Virus Process Using Biomass Spectrometer / Hela Kallel -- Inducing of Human IgE Antibodies by In Vitro Immunization / Hiroharu Kawahara -- Oxygen Uptake Rate (OUR) Estimation in High Density Mammalian Cell Perfusion Cultures / K. Konstantinov -- Use of Yeast Derived Nutrients for Cell Culture in Serum-Free Media / A. Marc -- Novel Approach to the Production of Plant-Derived Hydrolysates Yields Medium Supplements with Enhanced Performance in Cell Culture Systems / Shawn R. Smith -- Monitoring the Cell Size Distribution of Mammalian Cell Cultures Using On-Line Capacitance Measurements / Amine Kamen -- Biosimilarity of Recombinant Human EPO Products from CHO Cell Lines: A Carbohydrate Structural View / Harald S. Conradt Summary It has been predicted that nearly half of all human proteins are glycosylated indicating the significance of glycoproteins in human health and disease. For example, the glycans attached to proteins have emerged as important biomarkers in the diagnosis of diseases such as cancers and play a significant role in how pathogenic viruses gain entry into human cells. The study of glycoproteins has now become a truly proteomic science. In the last few years, technology developments including in silico methods, high throughput separation and detection techniques have accelerated the characterization of glycoproteins in cells and tissues. Glyco-engineering coupled to rapid recombinant protein production has facilitated the determination of glycoprotein structures key to exploring and exploiting their functional roles. Each chapter in this volume is written by experts in the field and together provide a review of the state of the art in the emerging field of glycoproteomics Analysis biomedische wetenschappen biomedicine Medicine (General) Geneeskunde (algemeen) Bibliography Includes bibliographical references and index Notes Print version record Subject Cell culture -- Congresses Animal cell biotechnology -- Congresses Animals -- cytology Biotechnology Cell Culture Techniques bioengineering. SCIENCE -- Biotechnology. Cell culture. Animal cell biotechnology. Biomédecine. Sciences de la vie. Animal cell biotechnology Cell culture Genre/Form proceedings (reports) Conference papers and proceedings Conference papers and proceedings. Actes de congrès. Form Electronic book Author Noll, Thomas. ISBN 9789048134199 9048134196 9789048134182 9048134188

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