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Title Cytokines and colony stimulating factors : methods and protocols / edited by Dieter Körholz and Wieland Kiess
Published Totowa, N.J. : Humana Press, ©2003


Description 1 online resource (xv, 478 pages) : illustrations
Series Methods in molecular biology ; v. 215
Methods in molecular biology (Clifton, N.J.) ; v. 215.
Contents Primary immunodeficiencies caused by defects of cytokines and cytokine receptors / V. Wahn -- Intracellular detection of T-cell cytokines. Differentiation of TH1 and TH2 cells / U. Banning and D. Korholz -- Analysis of cytokine profiles in human skin / J. Krutmann, R. Mang and S. Grether-Beck -- Intracytoplasmic detection of proinflammatory cytokines and chemokines in monocytes by flow cytometry / C. Schultz -- Flow-cytometric immune function methodology for human peripheral blood dendritic cells / K. Willmann -- Evaluation of the frequency of virus-specific CD8+ T cells by cytokine flow cytometry / B. Hoffmeister [and others] -- Detection of common cytokine and colony stimulating factor gene polymorphisms / T. Lehrnbecher and S.J. Chanock -- Apoptosis induction by TRAIL / A. Eggert, H. Sieverts and H. Walczak -- Angiogenic cytokines. Quantitative and functional analysis / A. Eggert and J.H. Maris -- Experimental strategies for combined suicide and immune cancer gene therapy. An overview / C.M. Kramm, T. Niehues and N.G. Rainov -- Gene therapy with plasmids encoding cytokine- or cytokine receptor -- IgG chimeric proteins / C.A. Piccirillo and G.J. Prud'homme -- Interferon-beta gene therapy for the treatment of arthritis / Y. Chernajovsky [and others] -- Gene gun-based in vivo gene transfer. Application to DNA vaccination / T. Sakai and K. Himeno -- Purification of the eucaryotic heat-shock proteins Hsp70 and gp96 / A. von Bonin, S.H. More and M. Breloer -- Genetic engineering of a recombinant fusion protein possessing an antitumor antibody fragment and a TNF-alpha moiety / J. Xiang and J.R. Gordon -- Genetic engineering of dendritic cells by adenovirus-mediated TNF-alpha gene transfer / J. Xiang and J. Wu -- Retroviral transfer of T-cell receptor genes into human peripheral blood lymphocytes / T.M. Clay and M.I. Nishimura -- Gene transfer to articular chondrocytes with recombinant adenovirus / G.D. Palmer [and others] -- Gene therapy of X-linked severe combined immunodeficiency / S. Hacein-Bey-Abina, G. de Saint Basile and M. Cavazzana-Calvo -- Gene transfer for generation of tumor and leukemia vaccines / D. Dilloo and A. Zibert -- Cytokine gene delivery into the central nervous system using intrathecally injected nonreplicative viral vectors / R. Furlan [and others] -- In vitro regulation of colony stimulating factor-mediated hematopoiesis in healthy individuals and patients with different types of myeloproliferative disease / T. Vraetz, P.D. Emanuel and C.M. Niemeyer -- In vitro generation of dendritic cells from cord blood CD34+ hematopoietic progenitors cells / B. Canque, M. Rosenzwajg and J.C. Gluckman -- Generation of transgenic T cells from human CD34+ cord blood cells / B. Verhasselt, E. Naessens and V. Stove -- Ex vivo expansion of umbilical cord blood cells on feeder layers / M. Bornhauser -- Ex vivo production of cord blood CD34+ derived myeloid precursors after serum-free static culture / S. Querol -- Ex vivo expansion of hematopoietic stem cells / B. Albella [and others] -- Large-scale ex vivo expansion of human megakaryocytes for clinical use / P. Lefebvre and I. Cohen -- Cytokine-mediated expansion of human NOD-SCID-repopulating cells / K. Tsuji, T. Ueda and Y. Ebihara -- Differentiation of human antigen-presenting dendritic cells from CD34+ hematopoietic stem cells in vitro / X.S. Ju and M. Zenke -- Dendritic cell development from mobilized peripheral blood CD34+ cells / D.M. Lathers, N. Achille and M.R. Young -- In vitro maturation of dendritic cells from blood progenitors / L. Matera and A. Galetto -- Generation of human type 1- and type 2-polarized dendritic cells from peripheral blood / P. Kalinski [and others] -- Preparation of human dendritic cells for tumor vaccination / M.R. Shurin -- Generation of leukemic dendritic cells from patients with acute myeloid leukemia / M. Mohty, B. Gaugler and D. Olive
Summary Enormous effort has been spent developing many new laboratory techniques for studying immune cells and the immune system, not only to increase fundamental understanding, but also to procure therapeutic success in a clinical setting. In Cytokines and Colony Stimulating Factors: Methods and Protocols, leading laboratory experts describe for the novice investigator a host of novel technologies and molecular techniques specifically designed to study cellular immunology and promote its gene therapy applications. Presented in step-by-step detail to ensure ready reproducibility, these protocols range from flow cytometric techniques to detect cytokines and growth factors in different specimens, to methods for generating and expanding dendritic cells and hematopoietic progenitors from different origins. Each proven method has been fine-tuned for experimental successs by the investigator who developed it, and includes notes on troubleshooting and avoiding potential pitfalls. Although the protocols require little previous experience, even highly skilled researchers will augment their experimental range with many novel timesaving techniques. Cutting-edge and highly practical, Cytokines and Colony Stimulating Factors: Methods and Protocols provides researchers a carefully evaluated set of powerful tools for successful experimental research on cytokines and their role in gene therapy
Bibliography Includes bibliographical references and index
Notes English
Print version record
In Springer Protocols
Subject Cytokines -- Laboratory manuals
Colony-stimulating factors (Physiology) -- Laboratory manuals
Colony-Stimulating Factors -- physiology
MEDICAL -- Immunology.
Colony-stimulating factors (Physiology)
Colony stimulating factors.
Wetenschappelijke technieken.
Genre/Form Handbooks and manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Körholz, Dieter
Kiess, W. (Wieland)
LC no. 2002027346
ISBN 9781592593453