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Title Plant cytogenetics : methods and protocols / edited by Shahryar F. Kianian, Penny M.A. Kianian
Published New York, NY : Humana Press, 2016


Description 1 online resource (xii, 198 pages) : illustrations
Series Methods in Molecular Biology ; 1429
Methods in molecular biology (Clifton, N.J.) ; 1429
Contents C-Banding of Plant Chromosomes -- Chromosome Painting by GISH and Multi-Color FISH -- Fluorescent In Situ Hybridization on Extended Chromatin Fibers for High-Resolution Analysis of Plant Chromosomes -- Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homologous Chromosomes -- Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH -- Immunolabeling and In Situ Labelling of Isolated Plant Interphase Nuclei -- Manipulation of Homologous Chromosome Recombination in Wheat -- Dissecting Plant Chromosomes by the Use of Ionizing Radiation -- Optical Nano-Mapping and Analysis of Plant Genomes -- Flow Sorting Plant Chromosomes -- Construction of BAC Libraries from Flow-Sorted Chromosomes -- The Chromosome Microdissection and Microcloning Technique -- Immunolocalization on Whole Anther Chromosome Spreads for Male Meiosis -- Mapping Recombination Initiation Sites Using Chromatin Immunoprecipitation -- Chromatin Immunoprecipitation to Study Plant Epigenome
Summary This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as recombination initiation sites and epigenomic marks. The methods described in Plant Cytogenetics: Methods and Protocols build on each other and provide, those new to the field, with a comprehensive platform to support their research endeavours, while also introducing advanced techniques to experienced researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Plant Cytogenetics: Methods and Protocols, is a valuable resource for anyone who is interested in the diverse and wonderfully complex field of cytogenetics
Notes Includes index
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Plant cytogenetics.
Plant chromosomes.
Cytogenetics -- methods
Cytogenetic Analysis -- methods
Botany & plant sciences.
Science -- Life Sciences -- Botany.
Plant chromosomes
Plant cytogenetics
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Kianian, Shahryar F., editor
Kianian, Penny M. Avoles, editor
LC no. 2016941227
ISBN 9781493936229