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Title Cytochrome P450 protocols / edited by Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano
Edition 3rd ed
Published Totowa, N.J. : Humana ; London : Springer [distributor], 2013


Description 1 online resource (xii, 319 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; v. 987
Methods in molecular biology (Clifton, N.J.) ; volume 987. 1064-3745
Contents Bioluminescent assays for cytochrome P450 enzymes / Douglas S. Auld, Henrike Veith, and James J. Cali -- Simultaneous determination of multiple CYP inhibition constants using a cocktail-probe approach / Michael Zientek and Kuresh Youdim -- High-throughput mass spectrometric cytochrome P450 inhibition screening / Kheng B. Lim, Can C. Ozbal, and Daniel B. Kassel -- Synthesis, characterization, and application of ¹³c-methyl isocyanide as an NMR probe of heme protein active sites / Christopher McCullough [and others] -- High-throughput fluorescence assay for cytochrome P450 mechanism-based Inactivators / Cesar Kenaan, Haoming Zhang, and Paul F. Hollenberg -- Identification of endogenous substrates of orphan cytochrome P450 enzymes through the use of untargeted metabolomics approaches / Qian Cheng and F. Peter Guengerich -- Genetic and mass spectrometric tools for elucidating the physiological function(s) of cytochrome P450 enzymes from Mycobacterium tuberculosis / Hugues Ouellet [and others] -- Escherichia coli expression-based approach for porphyrin substitution in heme proteins / Michael B. Winter, Joshua J. Woodward, and Michael A. Marletta -- Expression in Escherichia coli of a cytochrome P450 enzyme with a cobalt protoporphyrin IX porosthetic group / Wesley E. Straub [and others] -- Nanodiscs in the studies of membrane-bound cytochrome P450 enzymes / A. Luthra [and others] -- Rapid LC-MS drug metabolite profiling using bioreactor particles / Linlin Zhao, Besnik Bajrami, and James F. Rusling -- Fluorescence-based screening of cytochrome P450 activities in intact cells / M. Teresa Donato and M. José Gómez-Lechón -- Screening for cytochrome P450 reactivity with a reporter enzyme / Kersten S. Rabe and Christof M. Niemeyer -- High-throughput fluorescence assay of cytochrome P450 3A4 / Qian Cheng and F. Peter Guengerich -- Targeted protein capture for analysis of electrophile-protein adducts / Rebecca E. Connor [and others] -- DNA shuffling of cytochrome P450 enzymes / James B.Y.H. Behrendorff, Wayne A. Johnston, and Elizabeth M.J. Gillam -- Measurement of P450 difference spectra using intact cells / Wayne A. Johnston and Elizabeth M.J. Gillam -- DNA shuffling of cytochromes P450 for indigoid pigment production / Nedeljka N. Rosic -- P450 oxidoreductase : genotyping, expression, purification of recombinant protein, and activity assessments of wild-type and mutant protein / Vishal Agrawal and Walter L. Miller -- LICRED : a versatile drop-in vector for rapid generation of redox-self-sufficient cytochromes P450 / Federico Sabbadin, Gideon Grogan, and Neil C. Bruce -- Update on allele nomenclature for human cytochromes P450 and the human cytochrome P450 allele (CYP-allele) nomenclature database / Sarah C. Sim and Magnus Ingelman-Sundberg -- Simultaneous in vivo phenotyping of CYP enzymes / Sussan Ghassabian and Michael Murray -- Detection of regulatory polymorphisms : high-throughput capillary DNase I footprinting / Matthew Hancock and Elizabeth A. Shephard -- Isolation of mouse hepatocytes / Mina Edwards [and others] -- Highly efficient SiRNA and gene transfer into hepatocyte-like HepaRG cells and primary human hepatocytes : new means for drug metabolism and toxicity studies / Véronique Laurent [and others]
Summary Cytochromes P450 (CYPs) comprise a large superfamily of proteins that are of central importance in the detoxification or activation of a tremendous number of natural and synthetic hydrophobic xenobiotics, including many therapeutic drugs, chemical carcinogens and environmental pollutants. CYPs are important in mediating interactions between an organism and its chemical environment and in the regulation of physiological processes. Cytochrome P450 Protocols, Third Edition focuses on high-throughput methods for the simultaneous analysis of multiple CYPs, substrates or ligands. Although the emphasis is on CYPs of mammalian origin, it reflects an increasing interest in CYPs of bacterial species. Also included are chapters on cytochrome P450 reductase (the redox partner of CYPs) and the flavin-containing monooxygenases (FMOs), and metabolomic and lipidomic approaches for identification of endogenous substrates of CYPs ('de-orphanizing' CYP substrates). Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cytochrome P450 Protocols, Third Edition provides a wide range of techniques accessible to researchers in fields as diverse as biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics
Analysis cytochromen
Proteins and Enzymes
Eiwitten en enzymen
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Cytochrome P-450.
Cytochrome P-450 -- Laboratory manuals
Cytochrome P-450 Enzyme System
Cytochrome P-450
Physical Sciences & Mathematics.
Genre/Form Laboratory manuals
Form Electronic book
Author Phillips, Ian R. (Ian Randall), 1951-
Shephard, Elizabeth A. (Elizabeth Anne), 1950-
Ortiz de Montellano, Paul R.
ISBN 9781627033213