| Description |
1 online resource (xiii, 509 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1257 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1257. 1064-3745
|
| Contents |
Principles of cryopreservation / David E. Pegg -- Principles of cryopreservation by vitrification / Gregory M. Fahy and Brian Wowk -- Modeling and optimization of cryopreservation / James D. Benson -- Principles of freeze-drying / Gerald D.J. Adams, Isobel Cook, and Kevin R. Ward -- Use of in situ fourier transform infrared spectroscopy to study freezing and drying of cells / Willem F. Wolkers and Harriëtte Oldenhof -- Calorimetric analysis of cryopreservation and freeze-drying formulations / Wendell Q. Sun -- Measurement of intracellular ice formation kinetics by high-speed video cryomicroscopy / Jens O.M. Karlsson -- Laser scanning microscopy in cryobiology / Frank Stracke, Asger Kreiner-Møller, and Heiko Zimmermann -- Low-temperature electron microscopy : techniques and protocols / Roland A. Fleck -- Cryopreservation of semen from domestic livestock / Harald Sieme and Harriëtte Oldenhof -- Cryopreservation of mammalian oocytes / Victoria Keros and Barry J. Fuller -- Vitrification : a simple and successful method for cryostorage of human blastocysts / Juergen Liebermann -- Efficient cryopreservation of human pluripotent stem cells by surface-based vitrification / Julia C. Neubauer [and 3 others] -- Cryopreservation of Greenshell mussel (Perna canaliculus) sperm / Serean L. Adams [and 4 others] -- Membrane modification strategies for cryopreservation / Phillip H. Purdy and James K. Graham -- Sperm cleanup and centrifugation processing for cryopreservation / Harald Sieme and Harriëtte Oldenhof -- Cryopreservation of red blood cells / Johan W. Lagerberg -- Cord blood clinical processing, cryopreservation, and storage / Heidi Elmoazzen and Jelena L. Holovati -- Directional freezing for large volume cryopreservation / Joseph Saragusty -- Vitrification of heart valve tissues / Kelvin G.M. Brockbank [and 3 others] -- Cryopreservation of plant cell lines / Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack -- Writing standard operating procedures (SOPs) for cryostorage protocols : using shoot meristem cryopreservation as an example / Keith Harding and Erica E. Benson -- Freeze-drying of proteins / Baolin Liu and Xinli Zhou -- Freeze-drying of lactic acid Bbacteria / Fernanda Fonseca, Stéphanie Cenard, and Stéphanie Passot -- Freeze-drying of mammalian sperm / Levent Keskintepe and Ali Eroglu -- Freeze-drying of decellularized deart valve tissues / Willem F. Wolkers and Andres Hilfiker |
| Summary |
This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Cryopreservation of organs, tissues, etc. -- Methods
|
|
Freeze-drying -- Methods
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Cryopreservation -- methods
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Freeze Drying -- methods
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Materials science.
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Biotechnology.
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Technology & Engineering -- Material Science.
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Science -- Biotechnology.
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Cryopreservation of organs, tissues, etc.
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Freeze-drying
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| Genre/Form |
Methods (Music)
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Wolkers, Willem F., editor
|
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Oldenhof, Harriëtte, editor
|
| ISBN |
9781493921935 |
|
1493921932 |
|
1493921924 |
|
9781493921928 |
|
