Title Plant conservation biotechnology / edited by Erica E. Benson

Published London ; Philadelphia, PA : Taylor & Francis, ©1999

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Description 1 online resource (xxv, 309 pages) : illustrations Contents Cover -- Title -- Copyright -- Contents -- List of Figures -- Foreword -- Preface -- Acknowledgements -- Contributing Authors -- PART ONE Principles of Plant Conservation Biotechnology: Methods, Techniques and Procedures -- 1 An Introduction to Plant Conservation Biotechnology -- 1.1 Integrating biotechnology into conservation programmes -- 1.2 A general overview: how does biotechnology assist plant conservation? -- 1.3 Conservation biotechnology and the sustainable utilization of plant genetic resources -- 1.4 Conclusions and future prospects -- References -- 2 Molecular Approaches to Assessing Plant Diversity -- 2.1 Introduction -- 2.2 Molecular marker systems -- 2.3 Molecular markers in germplasm characterization -- 2.4 Molecular markers in systematics and population genetics -- 2.5 Prospects for molecular markers in biodiversity characterization -- References -- 3 Biotechnology in Plant Germplasm Acquisition -- 3.1 Introduction -- 3.2 Plant genetic resource conservation -- 3.2.1 In situ conservation -- 3.2.2 Ex situ conservation -- 3.2.3 Static and dynamic conservation -- 3.3 Acquisition procedures -- 3.3.1 Plant exploration -- 3.3.2 Plant exchange -- 3.4 Acquisition planning -- 3.4.1 Intellectual property rights -- 3.4.2 Quarantine regulations -- 3.5 Methods of acquisition -- 3.5.1 Seeds -- 3.5.2 Vegetative propagation -- 3.6 Documentation -- 3.7 Identity confirmation -- 3.8 Summary -- References -- 4 Tissue Culture Techniques in In Vitro Plant Conservation -- 4.1 Introduction -- 4.2 In vitro propagation -- 4.2.1 Germplasm acquisition -- 4.2.2 Selection of tissue for in vitro culture -- 4.2.3 Microbial contamination and disease indexing -- 4.2.4 Tissue culture media -- 4.2.5 Problems of culture establishment -- 4.2.6 Propagule multiplication (morphogenesis) -- 4.2.7 Plantlet development -- 4.3 Acclimatization of in vitro germplasm to in vivo conditions -- 4.4 In vitro culture recalcitrance -- 4.5 Embryo rescue -- 4.6 Use of plant tissue culture for germplasm storage -- 4.6.1 Slow (minimal) growth -- 4.6.2 Recovery of germplasm after storage -- 4.7 Facilities for plant tissue culture -- 4.8 Conclusions -- References -- 5 Phytosanitary Aspects of Plant Germplasm Conservation -- 5.1 Introduction -- 5.2 Safe movement of germplasm -- 5.2.1 Quarantine -- 5.2.2 International cooperation -- 5.3 International guidelines -- 5.4 Virus detection -- 5.4.1 Requirements for detection and diagnosis -- 5.4.2 Serological detection -- 5.4.3 Nucleic acid based assays -- 5.4.4 Detection based on more traditional methods -- 5.4.5 The significance of a test result -- 5.5 Production of pathogen-free plants -- 5.5.1 Heat therapy -- 5.5.2 Meristem tip culture -- 5.5.3 Chemotherapy -- 5.6 Conclusions -- References -- 6 Cryopreservation -- 6.1 Introduction -- 6.2 Principles of cryopreservation and germplasm preparation -- 6.2.1 Preparing germplasm for cryopreservation -- 6.2.2 Pre-treatments -- 6.3 Cryprotection -- 6.3.1 Traditional cryoprotection and controlled rate cooling -- 6.3.2 Vitrification -- 6.4 Freezing and long-term cryogenic storage -- 6.4.1 Controlled rate freezing -- 6.4.2 Rapid freezing and long-term storage -- 6.5 Post-cryopreservation recovery -- 6.6 Cryopreservation protocols: techniques and practical cons Summary Introduces biotechnological techniques which are currently used to conserve horticultural and crop plant germplasm, forest tree genetic resources, endangered plant species, and plant cell culture collections. Covers techniques and applications Bibliography Includes bibliographical references Notes English Print version record Subject Germplasm resources, Plant. Plant biotechnology. Cryopreservation of organs, tissues, etc. Biotechnology -- methods Plants -- genetics Cryopreservation TECHNOLOGY & ENGINEERING -- Agriculture -- General. Cryopreservation of organs, tissues, etc. Germplasm resources, Plant Plant biotechnology Planten. Natuurbehoud. Biotechnologie. Form Electronic book Author Benson, Erica E. LC no. 99488361 ISBN 0203792432 9780203792438 9780203484197 0203484193 9780748407460 0748407464 9781482273038 1482273039 1282778757 9781282778757 9786612778759 661277875X

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