Title Veterinary infection biology : molecular diagnostics and high-throughput strategies / edited by Mónica V. Cunha, Instituto Nacional de Investigação Agrária e Veterinária, Lisbon, Portugal ; Centro o de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisbon, Lisbon, Portugal ; João Inácio, Instituto Nacional de Investigação Agrária e Veterinária, Lisbon, Portugal, School of Pharmacy and Biomolecular Sciences, University of Brighton, United Kingdom

Published New York : Humana Press, [2015] ©2015

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Description 1 online resource (xix, 536 pages) : illustrations Series Methods in molecular biology, 1940-6029 ; 1247 Methods in molecular biology (Clifton, N.J.) ; v. 1247. 1064-3745 Contents Overview and challenges of molecular technologies in the veterinary microbiology laboratory -- Significance and integration of molecular diagnostics in the framework of veterinary practice -- Biosafety principles and practices for the veterinary diagnostic laboratory -- Veterinary biobank facility: Development and management for diagnostic and research purposes -- Biological specimen collection and processing for molecular analysis -- Validation of molecular diagnostic assays and quality assurance and control in the veterinary laboratory -- Molecular approaches to recognize relevant and emerging infectious diseases in animals -- Real-time reverse transcriptase PCR for the detection of bluetongue virus -- Nested and multiplex real-time PCR using dual-labeled probes: Detecting and discriminating mycobacterium tuberculosis complex members in cultures and animal tissues -- A real-time PCR assay for the diagnosis of gastrointestinal nematode infections of small ruminants -- Improved detection of mycobacterium bovis in bovine tissues using immunomagnetic separation approaches -- Detection of fish pathogens by loop-mediated isothermal amplification (LAMP) technique -- Direct detection of Theileria annulata in bovine blood samples using standard and isothermal DNA amplification approaches -- Reverse line blot hybridization with species-specific oligonucleotide probes: Application to piroplasm detection -- DNA microarray-based detection of multiple pathogens: Mycoplasma spp. And chlamydia spp -- In situ hybridization with labeled probes: Assessment of african swine fever virus in formalin-fixed paraffin-embedded tissues -- Fluorescence in situ hybridization for the tissue detection of bacterial pathogens associated with porcine infections -- Identification of animal pasteurellaceae by maldi-tof mass spectrometry -- Gold nanoparticles as a potential tool for diagnosis of fish diseases -- Nucleic-acid testing, new platforms and nanotechnology for point-of-decision diagnosis of animal pathogens -- Molecular typing tools: From pattern recognition to genome-based algorithms -- Characterization of campylobacter jejuni and campylobacter coli genotypes in poultry flocks by restriction fragment length polymorphism (RFLP) analysis -- Pulsed-field gel electrophoresis (PFGE): Application in population structure studies of bovine mastitis-causing streptococci -- Multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) using multiplex PCR and multicolor capillary electrophoresis: Application to the genotyping of brucella species -- Multilocus sequence typing (MLST): Markers for the traceability of pathogenic leptospira strains -- Single-nucleotide polymorphism discrimination using high-resolution melting analysis for the genotyping of bacillus anthracis -- Clustered regularly interspaced short palindromic repeats (CRISPRs) analysis of members of the mycobacterium tuberculosis complex -- Rapid microarray-based genotyping of chlamydia spp. Strains from clinical tissue samples -- Multiplexed genotyping of bacillus anthracis by Luminex xMap suspension array -- Next-generation sequencing in veterinary medicine: How can the massive amount of information arising from high-throughput technologies improve diagnosis, control, and management of infectious diseases? -- Impact of next-generation technologies on exploring socioeconomically important parasites and developing new interventions -- Functional genomics of tick vectors challenged with the cattle parasite Babesia bigemina -- Metagenomic approaches to disclose disease-associated pathogens: Detection of viral pathogens in honeybees -- Proteomics characterization of tick-host-pathogen interactions Bibliography Includes bibliographical references and index Notes Online resource; title from PDF title page (SpringerLink, viewed December 26, 2014) Subject Communicable diseases in animals -- Prevention -- Laboratory manuals Zoonoses -- Control -- Laboratory manuals Veterinary medicine -- Laboratory manuals Animals Communicable Disease Control Communicable Diseases -- veterinary High-Throughput Nucleotide Sequencing Molecular Diagnostic Techniques Zoonoses -- prevention & control Infectious & contagious diseases. Medical -- Infectious Diseases. Communicable diseases in animals -- Prevention Veterinary medicine Zoonoses -- Control Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Cunha, Monica V., editor. Inácio, João, editor. ISBN 9781493920044 1493920049

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