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Title Colorectal cancer : methods and protocols / edited by Steven M. Powell
Published Totowa, N.J. : Humana Press, ©2001


Description 1 online resource (xiii, 294 pages) : illustrations (some color)
Series Methods in molecular medicine ; 50
Methods in molecular medicine ; 50.
Contents Microdissection of histologic sections / C.A. Moskaluk -- Isolation of a purified epithelial cell population from human colon / J.K. Roche -- Xenografting human colon cancers / J.C. Harper, R.B. Adams and S.M. Powell -- Comparative genomic hybridization technique / W.e. El-Rifai and S. Knuutila -- Fluorescence in situ hybridization / S.D. Pack and Z. Zhuang -- Microsatellite analysis to assess chromosome 18q status in colorectal cancer / J. Jen -- The SURF technique / D. Shibata -- Microsatellite instability testing / Y.R. Parc and K.C. Halling -- Immunohistochemical analysis for hMLH1 and hMSH2 expression in colorectal cancer / K.C. Halling and P.C. Roche -- Mutation detection in colorectal cancers / J.M. Cunningham, D.J. Tester and S.N. Thibodeau -- Mutation detection inTGF-B receptors / W.M. Grady [and others] -- Direct analysis for familial adenomatous polyposis mutations / S.M. Powell -- I1307K mutation detection by allele-specific PCR in familial colorectal cancer / P.S. Chan and S.L. Gersen -- Microsatellite analysis of the insulin-like growth factor II receptor in colorectal carcinomas / R.F. Souza and S.J. Meltzer -- Molecular detection of Smad2/ Smad4 alterations in colorectal tumors / S. Thiagalingam -- Direct sequencing for juvenile polyposis gene SMAD4/DPC4 mutations / L.A. Aaltonen and S. Roth -- 17 direct sequencing for Peutz-Jeghers gene lkb1 (STK11) mutations / L.A. Aaltonen and E. Avizienyte -- 18 direct sequencing for cowden syndrome gene PTEN (MMAC1) mutations / L.A. Aaltonen, S. Roth and C. Eng -- Genetic analysis using microarrays / W.e. El-Rifai and S. Knuutila -- Cclooxygenase-2 (COX2) protein expression by western blotting / H.M. Kandil and R.N. DuBois -- The immunohistochemical method / L.A. Cerilli and H.F. Frierson -- Immunohistochemical detection and quantitation of cell surface receptors for prostanoids / R. Cosme and J.K. Roche -- Adhesion signaling through integrins / R.B. Adams and J.D. Rovin -- Electrophoretic mobility shift assay (EMSA) / M.F. Smith and S. Delbary-Gossart -- Transfection assays for transformation of model colonic cell lines / M.T. Worthington and R.Q. Luo -- Assessment of intestinal stem cell survival using the microcolony formation assay / K.S. Tustison, J. Yu and S.M. Cohn -- Gene transfer into the colonic mucosa / B. Ç̧oruh and T.T. Pizarro
Summary Colorectal cancer is a common and highly lethal cancer once spread whose treatment is improving as we gain a better understanding of its development. In Colorectal Cancer: Methods and Protocols, Steven M. Powell, MD, has assembled a hands-on collection of cutting-edge molecular methods for studying this solid tumor. These readily reproducible techniques range from gross microdissection of specimens to specific molecular analyses. Here both the novice and experienced researcher will find productive chromosomal analyses, including comparative genomic hybridization and FISH assays, and revealing gene expression analyses, including immunohistochemical assays, Western blotting, and microarray assays. Also presented are mutational analyses of colon tumors and blood samples to determine whether specific patients have inherited a significant predisposition to colorectal cancer. Each method is described in step-by-step detail by an established expert to ensure robust experimental results, and all are accompanied by detailed notes on pitfalls to avoid. Eminently practical, with each technique presented as if the expert in the field who had perfected it were guiding the investigator, Colorectal Cancer: Methods and Protocols provides cancer investigators with a gold standard collection of all the key genetic, cellular, and molecular biological methods for analyzing colon cancer today
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. MiAaHDL
Print version record
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
In Springer Protocols
Subject Colon (Anatomy) -- Cancer -- Diagnosis -- Laboratory manuals
Rectum -- Cancer -- Diagnosis -- Laboratory manuals
Colorectal Neoplasms -- pathology
Colorectal Neoplasms -- diagnosis
MEDICAL -- Oncology.
HEALTH & FITNESS -- Diseases -- Cancer.
Colon (Anatomy) -- Cancer -- Diagnosis
Rectum -- Cancer -- Diagnosis
Wetenschappelijke technieken.
Genre/Form Handbooks and manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Powell, Steven M.
ISBN 9781592590841