| Description |
1 online resource (xviii, 196 pages) : illustrations |
| Contents |
Front cover -- Preface to the Second Edition -- Preface to the First Edition -- Acknowledgments -- The Author -- Contents -- Chapter 1 -- The Scope of Microbiology -- I. Introduction -- II. Cells -- III. Binomial Nomenclature -- IV. Morphology -- V. Specific Microbial Groups -- A. Bacteria -- 1. The Gram Stain -- 2. External Features of Bacterial Cells -- 3. The Bacterial Interior -- 4. Bacterial Genetics -- B. Fungi -- 1. Molds -- 2. Yeasts -- C. Algae -- D. Protozoa -- E. Viruses -- 1. The Viral Infection Cycle -- 2. Retroviruses -- 3. Oncoviruses -- 4. Viruses and Gene Therapy -- F. Subviral Particles -- 1. Viroids -- 2. Satellite RNA -- 3. Prions -- VI. Taxonomy of Microorganisms -- VII. Microscopy -- A. Light Microscopy -- 1. Staining -- 2. Types of Light Microscopes -- B. Electron Microscopy -- VIII. Summary -- Chapter 2 -- Growth of Microorganisms -- I. Introduction -- A. Scientific Notation -- II. Growth of Bacteria -- A. Nutritional Requirements -- B. Environmental Requirements -- 1. Temperature -- 2. Atmosphere -- 3. pH -- C. Binary Fission and the Bacterial Growth Curve -- 1. Microbe Math -- 2. Characteristics of Bacterial Populations -- 3. The Growth Curve -- D. Continuous Growth -- E. Determining Bacterial Populations in Cultures -- 1. The Viable Plate Count -- 2. Filtration Methods -- 3. Total Cell Counts -- 4. Instrumental Methods -- F. Microorganisms as Tools -- III. Growing Other Microorganisms: Fungi, Protozoa, Algae, and Viruses -- A. Fungi -- B. Protozoa and Algae -- C. Viruses -- 1. Counting Viruses -- IV. Aseptic T echnique -- V. Safety Issues -- VI. Summary -- Chapter 3 -- Controlling Growth and Activities of Microorganisms -- I. Introduction -- II. Definitions -- III. Physical Methods of Control -- A. The Kinetics of Microbial Death -- B. Heat -- 1. Moist Heat -- 2. Dry Heat -- 3. Sterilizing Large Loads |
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C. Low Temperatures -- D. Radiation -- 1. Ionizing Radiation -- 2. Nonionizing Radiation -- E. Filtration -- 1. Depth Filters -- 2. Membrane Filters -- 3. Mechanisms of Filtration -- 4. Validation of Filtration Sterilization -- IV. Ensuring Sterility -- A. Overkill Method -- B. Bioburden Method -- C. Validation of Sterilization Processes -- D. Maintaining Sterility -- V. Chemical Methods of Control -- A. Definitions -- B. Factors Influencing Antimicrobial Chemical Agents -- C. Disinfectants -- 1. Characteristics of Specific Agents -- 2. Selection of Disinfectants -- 3. Validation of Disinfectants -- 4. Rotation of Disinfectants -- D. Sterilants -- 1. Characteristics of Specific Agents -- E. Antibiotics -- VI. Control of Microorganisms in High-Purity Water Systems -- A. The Need for Pure Water -- B. Water Purification Methods -- C. Biofilms -- VII. Summary -- Chapter 4 -- Cleanroom Facilities and Personnel Controls -- I. Introduction -- II. Certified Cleanrooms -- III. Bioclean Facilities -- Viable vs. Nonviable Particles -- IV. Clean Facilities -- General Considerations -- V. Unidirectional Air Flow -- VI. HEPA and ULPA Filters -- VII. Aseptic Fill Areas -- VIII. Barriers, Clean Zones, and Isolators -- A. Biological Safety Cabinets -- 1. Class I BSCs -- 2. Class II BSCs -- 3. Class III BSCs -- 4. Proper BSC Operation -- 5. BSC Certification -- B. Isolators -- C. Clean Benches -- IX. Biosafety Levels -- A. BSL4 Facilities -- B. Positive-Pressure Personnel Suits -- X. Cleanroom Garments -- A. Correct Gowning Procedures -- B. Sterile Gowning -- XI. Personnel Practices and Training -- XII. Personal Hygiene -- XIII. General Personnel Practices -- XIV. Summary -- Chapter 5 -- Detection and Enumeration of Microorganisms in the Cleanroom -- I. Introduction -- II. Overview of Microbiological Environmental Monitoring -- A. Validation -- B. Monitoring Techniques |
| Summary |
Written for the professional who has an immediate need for the information but has little or no training in the subject, Cleanroom Microbiology for the Non-Microbiologist, Second Edition introduces principles of microbiology. It explains the consequences of microbiological contamination, what contamination is all about, how microorganisms grow, and how they can be controlled. The author introduces the vocabulary of microbiology and the types, sources, control, and elimination of organisms encountered in the manufacture of sterile products. Beginning with a discussion of the various types of organisms, the text then covers applications for bacterial detection, avoidance of contamination, cleanroom design considerations, and validation of disinfection methods. New topics covered include: International cleanroom standards Application of rapid, automated methods for detecting and identifying microbial contaminants In-depth examination of the role of biofilms in pure water systems Increased coverage of production of therapeutic products derived from live tissues and cells |
| Bibliography |
Includes bibliographical references (pages 179-181) and index |
| Notes |
Print version record |
| Subject |
Clean rooms -- Microbiology.
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Sanitary microbiology.
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Microbiology.
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Environment, Controlled
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Microbiology
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Decontamination -- methods
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Decontamination -- standards
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microbiology.
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TECHNOLOGY & ENGINEERING -- Industrial Health & Safety.
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Microbiology
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Clean rooms -- Microbiology
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Sanitary microbiology
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| Form |
Electronic book
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| ISBN |
142002583X |
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9781420025835 |
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