Title Phage display in biotechnology and drug discovery / edited by Sachdev S. Sidhu

Published Boca Raton : CRC Press/Taylor & Francis, 2005

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Description 1 online resource (xviii, 748 pages) : illustrations Series Drug discovery series Drug discovery series. Contents Cover -- Dedication -- Foreword -- Preface -- Contents -- Contributors -- 1 Filamentous Bacteriophage Structure and Biology -- I. INTRODUCTION -- II. TAXONOMY AND GENETICS -- III. VIRAL GENE PRODUCTS -- III. A. Replication Proteins (pII and pX) -- III. B. Single-Stranded DNA Binding Protein (pV) -- III. C. Major Structural Protein (pVIII) -- III. D. Minor Structural Proteins (pIII, pVI, pVII, and pIX) -- III. E. Morphogenetic Proteins (pI, pIV, and pXI) -- IV. STRUCTURE OF THE VIRION -- IV. A. Overall Structural Organization -- IV. B. pVIII Structure -- IV. C. Distal End Structure -- IV. D. Proximal End -- IV. E. DNA Structure within the Viral Particle -- V. FILAMENTOUS BACTERIOPHAGE LIFE CYCLE -- V.A. Replication of Viral DNA -- V.B. Synthesis of Viral Proteins -- V.C. Viral Morphogenesis -- V.D. The Infection Process -- VI. PHAGE LIBRARY DIVERSITY -- VI. A. Efficiency as a Biological Strategy for Survival -- VI. B. Phage Population Diversity -- VII. BIOLOGICAL BOTTLENECKS: SOURCES OF LIBRARY CENSORSHIP -- VII. A. Protein Synthesis -- VII. B. Protein Insertion in the Inner Membrane -- VII. C. Protein Processing -- VII. D. Display in the Periplasm -- VII. E. Viral Morphogenesis -- VII. F. Infection Process -- VIII. QUANTITATIVE DIVERSITY ESTIMATION -- IX. IMPROVED LIBRARY CONSTRUCTION -- REFERENCES -- 2 Vectors and Modes of Display -- I. INTRODUCTION -- II. MOST DISPLAY VECTORS ARE BASED ON FILAMENTOUS PHAGE -- III. GENERAL CLONING VECTORS BASED ON FILAMENTOUS PHAGE -- IV. CLASSIFICATION OF FILAMENTOUS PHAGE DISPLAY SYSTEMS -- V. PHAGE f1-THE FIRST PHAGE-DISPLAY VECTOR -- VI. LOW DNA COPY NUMBER DISPLAY VECTORS BASED ON FD-TET -- VII. DIVERSITY OF TYPE 3 VECTORS -- VIII. TYPE 8 VECTORS: FIRST LESSONS -- IX. MOSAIC DISPLAY IN TYPE NN SYSTEMS -- X. MOSAIC DISPLAY IN PHAGEMID SYSTEMS -- XI. VECTORS FOR C-TERMINAL DISPLAY -- XII. PHAGE PROTEINS AS CONSTRAINING SCAFFOLDS -- XIII. CONCLUSION -- REFERENCES -- 3 Methods for the Construction of Phage-Displayed Libraries -- I. INTRODUCTION -- II. OLIGONUCLEOTIDE-DIRECTED MUTAGENESIS -- II. A. Strategies for the Design and Synthesis of Degenerate Codons -- II. B. Integration of Mutagenic Oligonucleotides into Phage Display Vectors -- III. RANDOM MUTAGENESIS -- III. A. In Vitro Chemical Mutagenesis -- III. B.E. coli Mutator Strains -- III. C. Error-Prone PCR -- IV. COMBINATORIAL INFECTION AND RECOMBINATION -- V. DNA SHUFFLING -- V.A. Methods for Improved Crossover Resolution -- V.B. Incorporation of Synthetic DNA -- REFERENCES -- 4 Selection and Screening Strategies -- I. INTRODUCTION -- II. GENERAL CONSIDERATIONS -- III. THE SELECTION PROCESS -- III. A. Washing -- III. B. Elution Methods -- III. C. Amplification -- tidtid6 Bibliography Includes bibliographical references and index Notes Print version record Subject Bacteriophages. Microbial biotechnology. Pharmaceutical biotechnology. Viral proteins. Affinity chromatography. Peptides -- Biotechnology Proteins -- Biotechnology Biotechnology. Bioengineering. Pharmaceutical chemistry. Bacteriophages Biotechnology Chemistry, Pharmaceutical Viral Proteins Chromatography, Affinity bioengineering. SCIENCE -- Life Sciences -- Biology. SCIENCE -- Life Sciences -- Microbiology. Pharmaceutical chemistry Biotechnology Bioengineering Affinity chromatography Bacteriophages Microbial biotechnology Peptides -- Biotechnology Pharmaceutical biotechnology Proteins -- Biotechnology Viral proteins Form Electronic book Author Sidhu, Sachdev S LC no. 2005048421 ISBN 0849359120 9780849359125

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