| Description |
1 online resource (xiii, 416 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; v. 523 |
|
Springer protocols |
|
Methods in molecular biology (Clifton, N.J.) ; volume 523
|
|
Springer protocols (Series)
|
| Contents |
Preparation of chromatin assembly extracts from preblastoderm Drosophila embryos / Edgar Bonte and Peter B. Becker -- Analysis of reconstituted chromatin using a solid-phase approach / Raphael Sandaltzopoulos and Peter B. Becker -- In vivo chromatin decondensation assays : molecular genetic analysis of chromatin unfolding characteristics of selected proteins / Sherry L. Winter, Philip Wong, and Mark G. Alexandrow -- DNA methyltransferase probing of chromatin structure within populations and on single molecules / Carolina Pardo [and others] -- Visualization of the expression of HMGN nucleosomal binding proteins in the developing mouse embryo and in adult mouse tissues / Takashi Furusawa and Michael Bustin -- Drug-induced premature chromosome condensation (PCC) protocols : cytogenetic approaches in mitotic chromosome and interphase chromatin / Eisuke Gotoh -- Analysis of DNA topology in yeast chromatin / Randall H. Morse -- Preparation and analysis of uniquely positioned mononucleosomes / Daria A. Gaykalova [and others] -- Monitoring DNA breaks in optically highlighted chromatin in living cells by laser scanning confocal microscopy / Michael J. Kruhlak, Arkady Celeste, and André Nussenzweig -- Methods to study transcription-coupled repair in chromatin / Hélène Gaillard, Ralf Erik Wellinger, and Andrés Aguilera -- Cytometric analysis of DNA damage : phosphorylation of histone H2AX as a marker of DNA double-strand breaks (DSBs) / Toshiki Tanaka [and others] -- Detection of DNA damage induced by topoisomerase II inhibitors, gamma radiation, and crosslinking agents using the comet assay / Lori A. Hazlehurst -- Analysis of genomic aberrations using comparative genomic hybridization of metaphase chromosomes / Melanie Carless -- In vitro replication assay with mammalian cell extracts / Wasia Rizwani and Srikumar P. Chellappan -- Investigation of genomic methylation status using methylation-specific and bisulfite sequencing polymerase chain reaction / Melanie Carless -- Biochemical analysis of arginine methylation in transcription / Marc Tini, Hina Naeem, and Joseph Torchia -- In vitro histone demethylase assays / Kenji Kokura and Jia Fang -- Histone chaperone as coactivator of chromatin transcription : role of acetylation / Shrikanth S. Gadad [and others] -- Histone deacetylase activity assay / Zhigang Yuan [and others] -- In vitro and in vivo assays for studying histone ubiquitination and deubiquitination / Ling Zhai, Heui-Yun Joo, and Hengbin Wang -- Detection of histone H3 phosphorylation in cultured cells and tissue sections by immunostaining / Jaya Padmanabhan -- Chromatin immunoprecipitation assays : analyzing transcription factor binding and histone modifications in vivo / Smitha Pillai, Piyali Dasgupta, and Srikumar P. Chellappan -- ChIP on Chip assays : genome-wide analysis of transcription factor binding and histone modifications / Smitha Pillai and Srikumar P. Chellappan -- ChIP-on-Chip analysis methods for Affymetrix tiling arrays / Sean J. Yoder and Steven A. Enkemann -- Non-radioactive assay methods for the assessment of telomerase activity and telomere length / Partha P. Banerjee and Shankar Jagadeesh -- Detecting ATM-dependent chromatin modification in DNA damage and heat shock response / Sandeep Misri, Shruti Pandita, and Tej K. Pandita |
| Summary |
This volume encompasses a wide array of techniques spanning from isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function |
| Analysis |
chromatine |
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chromatin |
|
ontogenie |
|
ontogeny |
|
genomen |
|
genomes |
|
chromosomen |
|
chromosomes |
|
cytogenetica |
|
cytogenetics |
|
biologische technieken |
|
biological techniques |
|
protocollen |
|
protocols |
|
Cytogenetics |
|
Cytogenetica |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Chromatin -- Structure
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Eukaryotic cells.
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Chromatin -- ultrastructure
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Eukaryotic Cells
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Eukaryotic cells
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| Genre/Form |
Laboratory manuals
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|
Laboratory manuals.
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Chellappan, Srikumar P.
|
| ISBN |
9781597451901 |
|
1597451908 |
|
9781607612520 |
|
1607612526 |
|
9781493956265 |
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1493956264 |
|
