| Description |
1 online resource (270 pages) |
| Series |
Developments in Organic Chemistry Ser |
|
Developments in Organic Chemistry
|
| Contents |
Front Cover -- ADVANCES IN CHEMICAL PROTEOMICS -- ADVANCES IN CHEMICAL PROTEOMICS -- Copyright -- Contents -- Contributors -- Biography -- 1 -- Concepts and principles -- 1 -- Introduction to proteomics for chemical biology -- 1.1 Multi-omics as a promising path to precision healthcare -- 1.2 The bottom-up proteomics workflow -- 1.2.1 Overview of proteomics -- 1.2.2 Sample preparation -- 1.2.2.1 Cell lysis -- 1.2.2.2 Total protein quantitation assay -- 1.2.2.3 Size-exclusion chromatography -- 1.2.2.4 Gel electrophoresis -- 1.2.2.5 Affinity capture -- 1.2.2.6 Protein digestion |
|
1.2.2.7 Offline peptide fractionation -- 1.2.3 Liquid chromatography -- 1.2.4 Mass spectrometry instrumentation and implementation in proteomics -- 1.2.4.1 Ion sources -- 1.2.4.2 Mass analyzers -- 1.2.4.3 Gas-phase peptide fragmentation and tandem mass spectrometry -- 1.2.4.4 Modes of spectral data acquisition in bottom-up proteomics -- 1.2.5 Bioinformatics of database search and peptide identification -- 1.2.5.1 Input pre-processing -- 1.2.5.2 In silico protein digestion -- 1.2.5.3 Peptide mass filtering -- 1.2.5.4 Peptide-spectrum matching and scoring |
|
1.3 Tools and strategies in chemical proteomics -- 1.3.1 Compound-centric versus activity-based probes -- 1.3.2 Chemical tagging on peptides -- 1.3.3 Metabolic labeling on proteins -- 1.4 Challenges and opportunities in chemical proteomics -- 1.4.1 Alternative bio-orthogonal affinity tags and trypsin-resistant affinity binders -- 1.4.2 Reactivity and selectivity of chemical probes -- 1.4.3 Bioinformatics for modification-specific proteomics -- 1.5 Conclusion -- References -- 2 -- Warheads for designing covalent inhibitors and chemical probes -- 2.1 Definitions -- 2.2 Foundational knowledge |
|
2.3 Recent developments -- 2.4 Points of view/opinions -- 2.4.1 Strategic considerations for the selection and optimization of the warheads -- 2.5 Summary and outlook -- References -- 3 -- Chemical derivatization of peptides for quantitative proteomics -- 3.1 Introduction -- 3.2 Quantitative proteomics concepts -- 3.2.1 Quantification methods -- 3.2.2 Data acquisition strategies -- 3.3 Labeling techniques for MS1-based quantification -- 3.3.1 General considerations -- 3.3.2 Isotope-coded affinity tag -- 3.3.3 Reductive dimethylation -- 3.3.4 Other MS1-based isotope labeling methods |
|
3.3.5 Labeling methods that do not use isotopic labeling -- 3.4 Labeling techniques for MS2- and MS3-based quantification -- 3.4.1 General considerations -- 3.4.2 Tandem Mass Tags -- 3.4.3 iTRAQ -- 3.4.4 Other isobaric tagging concepts based on reporter ion quantification -- 3.4.5 Quantification based on isobaric tagging and complementary fragment ions -- 3.4.6 Use of isobaric tags for sample amplification: application to single-cell proteomics -- 3.5 Achieving higher-order multiplexing by combining labeling strategies -- 3.6 Summary and outlook -- References |
|
4 -- Open search algorithms discover patterns of chemical modifications via LC-MS/MS |
| Notes |
Print version record |
| Subject |
Proteomics.
|
|
Biochemistry.
|
|
Chemistry, Organic.
|
|
Proteomics
|
|
Biochemistry
|
|
Chemistry, Organic
|
|
biochemistry.
|
|
organic chemistry.
|
|
Biochemistry
|
|
Chemistry, Organic
|
|
Proteomics
|
| Form |
Electronic book
|
| ISBN |
012821435X |
|
9780128214350 |
|
