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E-book

Title Lipid-protein interactions : methods and protocols / edited by Jörg H. Kleinschmidt
Edition 2nd ed
Published New York, NY : Humana Press, [2019]
©2019

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Description 1 online resource (xiv, 682 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 2003
Methods in molecular biology (Clifton, N.J.) ; v. 2003. 1064-3745
Contents Multiscale Modeling and Simulation Approaches to Lipid-Protein Interactions -- Quartz Crystal Microbalances as Tools for Probing Protein-Membrane Interactions -- Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid Membranes -- Thermodynamic Analysis of Protein-Lipid Interactions by Isothermal Titration Calorimetry -- Differential Scanning Calorimetry of Protein-Lipid Interactions -- Imaging and Force Spectroscopy of Single Transmembrane Proteins with the Atomic Force Microscope -- Kinetics of Insertion and Folding of Outer Membrane Proteins by Gel-Electrophoresis -- Optimized Negative-Staining Protocol for Lipid-Protein Interactions Investigated by Electron Microscopy -- Probing Heterogeneous Lipid Interactions with Membrane Proteins using Mass Spectrometry -- Protein Microarrays and Liposome: A Method for Studying Lipid-Protein Interactions -- Structural Investigations of Protein-Lipid Complexes using Neutron Scattering -- Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid Environment -- FTIR Analysis of Proteins and Protein-Membrane Interactions -- UV Resonance Raman Spectroscopy as a Tool to Probe Membrane Protein Structure and Dynamics -- Analyzing Transmembrane Protein and Hydrophobic Helix Topography by Dual Fluorescence Quenching -- Förster Resonance Energy Transfer as a Tool for Quantification of Protein-Lipid Selectivity -- A Guide to Tracking Single Membrane Proteins and Their Interactions in Supported Lipid Bilayers -- Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes -- Membrane Pore Formation by Peptides Studied by Fluorescence Techniques -- Folding and Insertion of [beta]-Barrel Membrane Proteins into Lipid Membranes by Site-Directed Fluorescence Spectroscopy -- EPR Techniques to Probe Insertion and Conformation of Spin-Labeled Proteins in Lipid Bilayers -- Studying Lipid-Protein Interactions with Electron Paramagnetic Resonance Spectroscopy of Spin-Labeled Lipids -- Solid-State NMR Approaches to Study Protein Structure and Protein-Lipid Interactions -- Solution NMR Spectroscopy for the Determination of Structures of Membrane Proteins in Lipid Environment -- Nanodiscs as a New Tool to Examine Lipid-Protein Interactions
Summary This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications. Additional protocols were added to examine lipid-protein interactions by mass spectrometry, to use protein microarrays to investigate large sets of various proteins, to study membrane protein dynamics by UV resonance Raman spectroscopy, to analyze peptide-induced pore formation in membranes, and to investigate folding and insertion of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Lipid-Protein Interactions: Methods and Protocols, Second Edition is an essential resource for all researchers who are interested in obtaining up-to-date and comprehensive information about membrane structure and function
Bibliography Includes bibliographical references and index
Subject Lipids.
Mass spectrometry.
Biochemistry.
Carrier proteins.
Lipids -- chemistry
Proteins -- chemistry
Lipids
Mass Spectrometry
Biochemistry
Membrane Lipids -- chemistry
Membrane Proteins -- chemistry
Carrier Proteins
lipid.
mass spectrometry.
biochemistry.
Carrier proteins
Biochemistry
Lipids
Mass spectrometry
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Kleinschmidt, Jörg H., editor.
ISBN 9781493995127
149399512X