| Description |
1 online resource (1 PDF file (xii, 405 pages)) : illustrations |
| Series |
Springer protocols |
|
Methods in molecular biology, 1940-6029 ; volume 2191 |
|
Springer protocols (Series)
|
|
Methods in molecular biology (Clifton, N.J.) ; v. 2191. 1064-3745
|
| Summary |
This book merges approaches in understanding the function of the light-gated ion channels known as channelrhodopsin together with methods addressing how channelrhodopsins can be used to address biomedical questions on a cellular or organismal level. Since the first molecular identification of channelrhodopsins, a broad range of tools have been created and new approaches developed to both better understand the molecular determinants of channelrhodopsin function as well as to use these and homologous proteins from a variety of species as tools to better understand physiological processes, which this volume addresses. Additionally, channelrhodopsins have become instrumental as a potential treatment for disease states. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Part I: Basic Science 1. Molecular Dynamics Simulations of Channelrhodopsin Chimera, C1C2 Monika R. VanGordon 2. Computing Potential of the Mean Force Profiles for Ion Permeation through Channelrhodopsin Chimera, C1C2 Chad Priest, Monika R. VanGordon, Caroline Rempe, Mangesh Chaudhari, Mark Stevens, Steve Rick, and Susan B. Rempe 3. Nanodisc Reconstitution of Channelrhodopsins Heterologously Expressed in Pichia pastoris for Biophysical Investigations Maria Walter and Ramona Schlesinger 4. Characterizing Channelrhodopsin Channel Properties via Two-Electrode Voltage Clamp and Kinetic Modeling Lindsey Prignano, Lauren Herchenroder, and Robert E. Dempski Part II: Cells 5. Charge Transport by Light-Activated Rhodopsins Determined by Electrophysiological Recordings Tamara Hussein and Christian Bamann 6. Probing Channelrhodopsin Electrical Activity in Algal Cell Populations Oleg A. Sineshchekov, Elena G. Govorunova, and John L. Spudich 7 |
|
Two-Photon Optogenetic Stimulation of Drosophila Neurons Mehmet Fişek and James M. Jeanne 8. Probing Synaptic Signaling with Optogenetic Stimulation and Genetically-Encoded Calcium Reporters Gabriel B. Borja, Himali Shroff, Hansini Upadhyay, Pin W. Liu, Valeriya Baru, Yung-Chih Cheng, Owen B. McManus, Luis A. Williams, Graham T. Dempsey, and Christopher A. Werley 9. Optogenetics to Interrogate Neuron-Glia Interactions in Pups and Adults Chloé Habermacher, Blandine Manot-Saillet, Domiziana Ortolani, Fernando C. Ortiz, and Maria Cecilia Angulo 10. Chronic Optogenetic Pacing of Human Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissues Marc Dwenger, William J. Kowalski, Hidetoshi Masumoto, Takeichiro Nakane, and Bradley B. Keller Part III: Animals 11. Patterned Optogenetic Stimulation Using a DMD-Projector Aanchal Bhatia, Sahil Moza, and Upinder S. Bhalla 12. Selecting Channelrhodopsin Constructs for Optimal Visual Restoration in Differing Light Conditions Tushar H |
|
Ganjawala and Zhuo-Hua Pan 13. Recording Channelrhodopsin-Evoked Field Potentials and Startle Responses from Larval Zebrafish Yagmur Idil Ozdemir, Christina A. Hansen, Mohamed A. Ramy, Eileen L. Troconis, Lauren D. McNeil, and Josef G. Trapani 14. Automated Functional Screening for Modulators of Optogenetically-Activated Neural Responses in Living Organisms Ross C. Lagoy, Eric Larsen, and Dirk R. Albrecht 15. Optogenetic Interrogation of ChR2-Expressing GABAergic Interneurons after Transplantation into the Mouse Brain Muhammad N. Arshad, Gloster B. Aaron, and Janice R. Naegele 16. Application of Targeting-Optimized Chronos for Stimulation of the Auditory Pathway Antoine Huet and Vladan Rankovic 17. Channelrhodopsins for Cell-Type Specific Illumination of Cardiac Electrophysiology Marbely C. Fernández, Ramona A. Kopton, Ana Simon Chica, Josef Madl, Ingo Hilgendorf, Callum M. Zgierski-Johnston, and Franziska Schneider-Warme 18 |
|
Optogenetic Control of Cardiac Autonomic Neurons in Transgenic Mice Angel Moreno, Grant Kowalik, David Mendelowitz, and Matthew W. Kay 19. Dissecting Mechanisms of Motivation within the Nucleus Accumbens Using Optogenetics Shannon L. Cole and Jeffrey J. Olney 20. Optogenetic Stimulation of the Central Amygdala Using Channelrhodopsin Anna S. Knes, Charlotte M. Freeland, and Mike J.F. Robinson 21. Optical Manipulation of Perfused Mouse Heart Expressing Channelrhodopsin-2 in Rhythm Control Xi Wang and Yue Cheng 22. Chronic Optogenetic Stimulation in Freely-Moving Rodents Thiago C. Moulin |
|
Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force. WlAbNL |
| Subject |
Life sciences.
|
|
Proteins.
|
|
Microbiology.
|
|
Channelrhodopsins
|
|
Biological Science Disciplines
|
|
Microbiology
|
|
biological sciences.
|
|
protein.
|
|
microbiology.
|
|
Microbiology (non-medical).
|
|
Proteins.
|
|
Science -- Life Sciences -- Biology -- Microbiology.
|
|
Science -- Life Sciences -- Biochemistry.
|
|
Life sciences.
|
|
Microbiology.
|
|
Proteins.
|
| Genre/Form |
Laboratory manuals.
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Dempski, Robert E., editor
|
| ISBN |
9781071608302 |
|
1071608304 |
|
