Title T-cell trafficking : methods and protocols / edited by Federica M. Marelli-Berg, Sussan Nourshargh

Published Totowa, N.J. : Humana ; London : Springer [distributor], ©2010

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Description 1 online resource Series Methods in molecular biology ; 616 Methods in molecular biology (Clifton, N.J.) ; 616 Contents How T cells find their way around / Alf Hamann -- Live imaging of leukocyte-endothelium interactions / Olga Barreiro, Francisco Sánchez-Madrid, and María Yáñez-Mó -- Leucocyte adhesion under haemodynamic flow conditions / Charlotte Lawson, Marlene Rose, and Sabine Wolf -- Influence of stromal cells on lymphocyte adhesion and migration on endothelial cells / Helen M. McGettrick [and others] -- Discriminating between the paracellular and transcellular routes of diapedesis / Jaime Millán, Eva Cernuda-Morollón, and Severine Gharbi -- Monitoring RhoGTPase activity in lymphocytes / Marouan Zarrouk, David Killock, and Aleksandar Ivetic -- Visualisation of signalling in immune cells / Leo M. Carlin [and others] -- Methods for quantitation of leukocyte chemotaxis and fugetaxis / Fabrizio Vianello, Elda Righi, and Mark C. Poznansky -- Analysis of CXCR3 and atypical variant expression and signalling in human T lymphocytes / Anna Korniejewska, Malcolm Watson, and Stephen Ward -- Transfection of indoleamine 2,3 dioxygenase in primary endothelial cells / Petros X.E. Mouratidis and Andrew J.T. George -- Visualisation of lymphoid organ development / Henrique Veiga-Fernandes [and others] -- Single-cell analysis of cytotoxic T cell function by intravital multiphoton microscopy / Thorsten R. Mempel -- Imaging interactions between the immune and cardiovascular systems in vivo by multiphoton microscopy / Owain R. Millington [and others] -- Applying an adaptive watershed to the tissue cell quantification during T-cell migration and embryonic development / D. Zhu [and others] -- Identifying homing interactions in T-cell traffic in human disease / Patricia F. Lalor, Stuart M. Curbishley, and David H. Adams -- Tracking antigen-experienced effector T cells in vitro and in vivo / Claire L. Gorman [and others] -- Preclinical testing of strategies for therapeutic targeting of human T-cell trafficking in vivo / Caroline Coisne and Britta Engelhardt Summary In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explore how the development of novel and cutting-edge techniques, particularly in the field of real-time imaging and genetic manipulation, have led to an increased understanding of lymphocyte trafficking. Written by internationally recognized experts in their respective fields, chapters provide state-of-the-art protocols to study lymphocyte migration and T-cell: endothelial cell interactions in vitro, address various approaches used for direct visualization of the development of the lymphoid system, lymphocyte recirculation, and effector responses in experimental models in vivo, and explore lymphocyte migration and inflammation in the human system. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, T-Cell Trafficking: Methods and Protocols is an essential manual for newcomers in this ever-expanding and exciting area of research, as well as a valuable addition to more specialized laboratories Analysis t cellen t lymphocytes immunologie immunology geneeskunde medicine biomedische wetenschappen biomedicine laboratoriummethoden laboratory methods protocollen protocols Medicine (General) Geneeskunde (algemeen) Bibliography Includes bibliographical references and index Notes English Print version record Subject T cells. Cell differentiation -- Molecular aspects. Lymphocytes -- Metabolism -- Research Cells -- Motility -- Research Lymphocytes. Life sciences. Cell physiology. Physical sciences. Physiology. Leucocytes. Blood cells. Immune system. Blood. Cells. Anatomy. Lymphocytes Biological Science Disciplines Cell Physiological Phenomena Locomotion Natural Science Disciplines Movement Leukocytes, Mononuclear T-Lymphocytes Cell Movement Physiology Phenomena and Processes Physiological Phenomena Disciplines and Occupations Leukocytes Blood Cells Immune System Blood Hemic and Immune Systems Cells Anatomy Thymocytes Immune System Phenomena Animal Structures biological sciences. physical sciences. physiology. blood (animal material) anatomy. Physiology Physical sciences Lymphocytes Life sciences Leucocytes Immune system Cells Cell physiology Blood cells Blood Anatomy Cell differentiation -- Molecular aspects T cells Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Marelli-Berg, Federica M. Nourshargh, Sussan. ISBN 9781607614616 1607614618

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