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Title Programmed cell death : methods and protocols / edited by Hamsa Puthalakath and Christine J. Hawkins
Published New York, NY : Humana Press, 2016


Description 1 online resource (xi, 291 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume no. 1419
Methods in molecular biology (Clifton, N.J.) ; v. 1419, 1064-3745
Contents Detection of Apoptotic Versus Autophagic Cell Death by Flow Cytometry -- In Vivo Apoptosis Imaging Using Site-Specifically 68Ga-Labeled Annexin V -- Detection of Active Caspases during Apoptosis Using Fluorescent Activity-Based Probes -- Detection of Initiator Caspase Induced Proximity in Single Cells by Caspase Bimolecular Fluorescence Complementation -- In Vitro Use of Peptide Based Substrates and Inhibitors of Apoptotic Caspases -- Experimental In Vivo Sepsis Models to Monitor Immune Cell Apoptosis and Survival in Laboratory Mice -- Analysis of Cell Death Induction in Intestinal Organoids In Vitro -- In Vitro Differentiation of Mouse Granulocytes -- Hydrodynamic Injection as a Method of Gene Delivery in Mice: A Model of Chronic Hepatitis B Virus Infection -- Isolation of Cardiomyocytes and Cardiofibroblasts for Ex-Vivo Analysis -- Detection of Cell Death in Drosophila Tissues -- Methods to Study Plant Programmed Cell Death -- Modeling Metazoan Apoptotic Pathways in Yeast -- Characterizing Bcl-2 Family Protein Conformation and Oligomerization Using Cross-Linking and Antibody Gel-Shift in Conjunction with Native PAGE -- Using Förster-Resonance Energy Transfer to Measure Protein Interactions between Bcl-2 Family Proteins on Mitochondrial Membranes -- Preparing Samples for Crystallization of Bcl-2 Family Complexes -- Screening Strategies for TALEN Mediated Gene Disruption -- Using CRISPR/Cas9 Technology for Manipulating Cell Death Regulators -- Lentiviral Vectors to Analyze Cell Death Regulators -- Proteomic Profiling of Cell Death: Stable Isotope Labeling and Mass Spectrometry Analysis
Notes Includes index
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed April 25, 2016)
Subject Cell death -- Laboratory manuals
Cell Death
Cellular biology (cytology)
Science -- Life Sciences -- Biology -- Molecular Biology.
Cell death
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Puthalakath, Hamsa, editor.
Hawkins, Christine J., editor.
ISBN 9781493935819