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Title Autophagy : methods and protocols / edited by Nicholas Ktistakis and Oliver Florey
Published New York, NY : Humana Press : Springer, [2019]


Description 1 online resource (xvii, 741 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 1880
Methods in molecular biology (Clifton, N.J.) ; v. 1880. 1064-3745
Contents Recombinant expression, purification, and assembly of p62 filaments / Abul K. Tarafder, Audrey Guesdon, Tanja Kuhm, and Carsten Sachse -- Structural studies of autophagy-related proteins / Melanie Schwarten, Oliver H. Weiergr€aber, Dusˇan Petrović,Birgit Strodel, and Dieter Willbold -- Structural studies of mammalian autophagy lipidation complex / Kazuto Ohashi, Chinatsu Otomo, Zoltan Metlagel, and Takanori Otomo -- Structural studies of selective autophagy in yeast / Akinori Yamasaki, Yasunori Watanabe, and Nobuo N. Noda -- Biophysical studies of LC3 family proteins / Javier H. Herva s, Zurine Anton, and Alicia Alonso -- Reconstituting autophagy initiation from purified components / Peter Mayrhofer and Thomas Wollert -- Cell-free reconstitution of autophagic membrane formation / Min Zhang and Liang Ge -- Use of peptide arrays for identification and characterization of LIR motifs / Mads Skytte Rasmussen, Asa Birna Birgisdottir, and Terje Johansen -- Studying autophagic lysosome reformation in cells and by an in vitro reconstitution System / Yang Chen, Qian Peter Su, and Li Yu -- Formation of autophagosomes coincides with relaxation of membrane curvature / Jaime Agudo-Canalejo and Roland L. Knorr -- Studies of receptor-Atg8 interactions during selective autophagy / Christine Abert and Sascha Martens -- Correlative light and electron microscopy of autophagosomes / Sigurdur Gudmundsson, Jenny Kahlhofer, Nastassia Baylac, Katri Kallio, and Eeva-Liisa Eskelinen -- Improved electron microscopy fixation methods for tracking autophagy-associated membranes in cultured mammalian cells / Ritsuko Arai and Satoshi Waguri -- Three-color simultaneous live imaging of autophagy-related structures / Hiroyuki Ueda, Ouin Kunitaki, and Maho Hamasaki -- Correlative live-cell imaging and super-resolution microscopy of autophagy / Eleftherios Karanasios -- Methods for imaging autophagosome dynamics in primary neurons / Audrey Dong, Vineet Vinay Kulkarni, and Sandra Maday -- Imaging autophagy in hiPSC-derived midbrain dopaminergic neuronal cultures for parkinson's disease research / Petros Stathakos, Natalia Jimenez-Moreno, Lucy Crompton, Paul Nistor, Maeve A. Caldwell, and Jon D. Lane -- Correlative light and electron microscopy to analyze LC3 proteins in Caenorhabditis elegans embryo / Céline Largeau and Renaud Legouis -- Imaging noncanonical autophagy and LC3-associated phagocytosis in cultured cells / Elise Jacquin, Katherine Fletcher, and Oliver Florey -- Measurement of bulk autophagy by a cargo sequestration assay / Nikolai Engedal, Morten Luhr, Paula Szalai, and Per O. Seglen -- Methods to detect loss of lysosomal membrane integrity / Sonja Aits -- Monitoring of autophagy and cell volume regulation in kidney epithelial cells in response to fluid shear stress / Maria M. Lazari, Idil Orhon, Patrice Codogno, and Nicolas Dupont -- Identification and regulation of multimeric protein complexes in autophagy via SILAC-based mass spectrometry approaches / Stéphanie Kaeser-Pebernard, Britta Diedrich, and Jorn Dengjel -- Identification and validation of novel autophagy regulators using an endogenous readout siGENOME screen / Maria New, Tim Van Acker, Ming Jiang, Rebecca Saunders, Jaclyn S. Long, Jun-Ichi Sakamaki, Kevin M. Ryan, Michael Howell, and Sharon A. Tooze -- Autophagy pathway mapping to elucidate the function of novel autophagy regulators identified by high-throughput screening / Martina Wirth and Sharon A. Tooze -- In vitro screening platforms for identifying autophagy modulators in mammalian cells / Elena Seranova, Carl Ward, Miruna Chipara, Tatiana R. Rosenstock, and Sovan Sarkar -- Automated detection of autophagy response using single cell-based microscopy assays / Amelie J. Mueller and Tassula Proikas-Cezanne -- Methods for the study of entotic cell death / Jens C. Hamann, Sung Eun Kim, and Michael Overholtzer -- MHC class I internalization via autophagy proteins / Monica Loi, Laure-Anne Ligeon, and Christian Mu¨nz -- Analysis of autophagy for liver pathogenesis / Nazmul Huda, Hui Zou, Shengmin Yan, Bilon Khambu, and Xiao-Ming Yin -- Autophagy in 3D in vitro and ex vivo cancer models / Carlo Follo, Dario Barbone, William G. Richards,Raphael Bueno, and V. Courtney Broaddus -- Autophagy in platelets / Meenakshi Banerjee, Yunjie Huang, Madhu M. Ouseph, Smita Joshi, Irina Pokrovskaya, Brian Storrie, Jinchao Zhang, Sidney W. Whiteheart, and Qing Jun Wang -- Methods to image macroautophagy in the brain in vivo / Xigui Chen, Kanoh Kondo, and Hitoshi Okazawa -- Measuring nonselective and selective autophagy in the liver / Takashi Ueno and Masaaki Komatsu -- Measuring autophagy in pancreatitis / Alejandro Ropolo, Daniel Grasso, and Maria I. Vaccaro -- Characterization of the "Autophagic Flux" in prostate cancer tissue biopsies by LC3A/LAMP2a immunofluorescence and confocal microscopy / D. Kalamida, A. Giatromanolaki, and M. I. Koukourakis -- Methods to determine the role of autophagy proteins in C. elegans aging / Sivan Henis-Korenblit and Alicia Meléndez -- Investigating non-selective autophagy in Drosophila / Szabolcs Taká ts, Sarolta Toth, Gyo˝zo˝ Szenci, and Gá bor Juhá sz -- Imaging the dynamics of mitophagy in live cells / Andrew S. Moore and Erika L. F. Holzbaur -- Triggering mitophagy with photosensitizers / Cheng-Wei Hsieh and Wei Yuan Yang -- Investigating mitophagy and mitochondrial morphology in vivo using mito-QC : a comprehensive guide / Thomas G. McWilliams and Ian G. Ganley -- Assays to monitor mitophagy in Drosophila / Panagiotis Tsapras, Anne-Claire Jacomin, and Ioannis P. Nezis -- Mitophagy dynamics in Caenorhabditis elegans / Konstantinos Palikaras, Eirini Lionaki, and Nektarios Tavernarakis -- Methods for studying mitophagy in yeast / Panagiota Kolitsida and Hagai Abeliovich -- Measuring antibacterial autophagy / Keith B. Boyle and Felix Randow -- Quantitative phosphoproteomics of selective autophagy receptors / Thomas Juretschke, Petra Beli, and Ivan Dikic -- Analysis of chaperone-mediated autophagy / Y. R. Juste and A. M. Cuervo -- Interactive autophagy : monitoring a novel form of selective autophagy by macroscopic observations / Jana Petri and Roland L. Knorr
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed January 7, 2019)
Subject Cell death -- Laboratory manuals
Apoptosis -- Laboratory manuals
Cell death
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Ktistakis, Nicholas T., editor
Florey, Oliver, editor
ISBN 9781493988730