Title Therapeutic applications of ribozymes / edited by Kevin J. Scanlon

Published Totowa, N.J. : Humana Press, ©1998

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Description 1 online resource (xiv, 462 pages) : illustrations Series Methods in molecular medicine ; 11 Methods in molecular medicine ; 11. 1543-1894 Contents Small efficient hammerhead ribozymes -- The hairpin ribozyme: discovery and development for gene therapy -- Molecular manipulations of the catalytic RNAs from the human hepatitis delta virus -- The design, production, and validation of an anti-HIV type 1 ribozyme -- Methods for Treating HIV by Gene therapy using ribozymes -- An expression vector for multiple ribozymes -- Viral RNA targets for ribozymes: influenza A virus -- Hammerhead ribozyme-mediated cleavage of hepatitis B virus RNA -- Bovine leukemia virus ribozymes -- Use of ribozymes to specifically alter the MDR-1 phenotype -- Adenoviral-mediated anti-H-ras ribozyme for the treatment of bladder cancer -- Tissue-specific delivery of an anti-H-ras ribozyme against malignant melanoma -- Ribozymes as a novel approach for the treatment of human pancreatic carcinoma -- K-ras ribozyme for lung cancer -- Anti-c-erb-B-2 Ribozyme for breast cancer -- Ribozymes: applications in cancer metastasis research -- Preformed ribozymes: Strategies for design and in vivo application as anticytokine agents -- Folate-polylysine-mediated delivery of a multiunit anti-BCR/ABL ribozyme to BCR/ABL-transformed 32D cells -- Ribozymes targeted against AML1/MTG8: evaluation of ribozyme efficiency in a cell-free system and transfection with cationic liposome -- Ribozyme targeting of the growth factor pleiotrophin -- Ribozyme-mediated inhibition of cell proliferation: a model for identifying and refining a therapeutic ribozyme -- Inhibition of 13APP synthesis by ribozymes and oligonucleotides in a coupled reticulocyte transcription translation system -- Selective modification of fatty acid synthesis pathway by expression of an acetyI-CoA carboxylase-specific ribozyme gene -- In vivo knockout of a tissue-specific gene by synthetic ribozymes -- Ribozyme-mediated downregulation of gene expression in transgenic mice -- Targeted cleavage of RNA using external guide sequences and eukaryotic RNase P -- A PCR-based approach to hammerhead ribozyme synthesis-- PCR-based approach to hammerhead ribozyme synthesis -- Measurement of ribozyme activity using RNase protection Summary In Therapeutic Applications of Ribozymes, expert laboratory scientists describe in detail their methodologies for constructing ribozymes designed to elucidate the role of specific genes as key routes to the development of novel therapies for a wide variety of diseases. The authors review the many sites targeted with ribozymes in various diseases and provide specific accounts of the practical techniques required for the proper use of ribozymes in these systems. Their cutting-edge protocols demonstrate how to achieve ribozyme expression in distinct cellular systems, the preparation and use of high-efficiency ribozyme DNA or RNA delivery, and the studies required to prove the efficacy of ribozyme-mediated inhibition, with its concomitant effect on phenotypic parameters. Therapeutic Applications of Ribozymes contains all the practical detail necessary to realize the high promise of ribozyme technology as a significant, widely used methodology. Focused on ribozyme targets and the novel associated technology, the book immediately becomes the leading resource for all those seeking to transform this new category of therapeutic agent into efficacious clinical practice Analysis ribozymen ribozymes geneesmiddelenbehandeling drug therapy Pharmacology Farmacologie Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Subject Catalytic RNA -- Therapeutic use Catalytic RNA -- Laboratory manuals Gene therapy. Methodology. Non-coding RNA. Genetic engineering. Biological products -- Therapeutic use. Enzymes. RNA. Genetics -- Technique. Therapeutics. Nucleic acids. Genetic Therapy Methods RNA, Catalytic Investigative Techniques RNA, Untranslated Genetic Engineering Biological Therapy Enzymes Enzymes and Coenzymes RNA Genetic Techniques Therapeutics Analytical, Diagnostic and Therapeutic Techniques and Equipment Nucleic Acids Chemicals and Drugs Nucleic Acids, Nucleotides, and Nucleosides methodology. enzyme. treating (health care function) Therapeutics RNA Nucleic acids Non-coding RNA Methodology Genetics -- Technique Genetic engineering Gene therapy Enzymes Biological products -- Therapeutic use Catalytic RNA Catalytic RNA -- Therapeutic use Ribozymes. Therapeutische werking. Wetenschappelijke technieken. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Scanlon, Kevin J., 1947- ISBN 9781592595952 1592595952

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