Title Membrane transporters : methods and protocols / edited by Qing Yan

Published Totowa, NJ : Humana Press, 2003

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Description 1 online resource (xii, 369 pages) : illustrations Series Methods in molecular biology ; 227 Methods in molecular biology (Clifton, N.J.) ; v. 227. Contents Pharmacogenomics of membrane transporters an overview / Q. Yan -- The iubmb-endorsed transporter classification system / W. Busch and M.H. Saier, Jr. -- Bioinformatics and data integration in membrane transporter studies / Q. Yan -- DNA microarrays to analyze gene expression in animals with altered transporter expression / G.W. Miller -- Microarray data analysis in studies of membrane transporters / D. Hu -- Laser capture microdissection to examine transporter expression in specific cell regions / O. Rho and G.W. Miller -- Site-directed mutagenesis in the study of membrane transporters / R.M. Ryan, A.D. Mitrovic and R.J. Vandenberg -- Fluorescence techniques for studying membrane transport proteins: The p-glycoprotein multidrug transporter / F.J. Sharom [and others] -- Reconstitution of membrane transporters / F.J. Sharom and P.D. Eckford -- Equilibrium binding and transport studies / A.M. Ojeda [and others] -- Electrophysiological studies of ion channels and therapeutic potential with adenovirus-mediated gene transfer / J. Qu, T.V. Pham and M.N. Obreztchikova -- Antibody production and immunocytochemical localization of amino acid transporters / D.V. Pow, R. Sullivan and H. Scott -- Xenopus laevis oocytes / S. Broer -- Measurement of intracellular ph / F.B. Loiselle and J.R. Casey -- Small-angle x-ray scattering studies of membrane transporter peripheral components and soluble domains / B.H. Shilton -- Membrane protein nmr studies / S.J. Opella -- Analysis of soluble sugar permease domains by solution nmr / T.A. Wilkinson and Y. Chen -- Molecular modeling and molecular dynamics simulations of membrane transporter proteins / D.S. Dwyer -- Imaging plasma membrane proteins by confocal microscopy / S.W. Tsang and P.M. Haney Summary Membrane transporters and channels play key roles in drug absorption and distribution, influence drug efficacy and toxicity, and are the target of about 30% of currently marketed drugs. In Membrane Transporters: Methods and Protocols, a panel of recognized basic and clinical investigators-many world-famous-describe their proven, cutting-edge methodologies for studying these important proteins. Treated in step-by-step detail, these techniques take advantage of all the latest developments in biomedical research, including pharmacogenomics, bioinformatics, and microarray technologies. The authors explain databases and tools for bioinformatics analysis, provide practical guidelines for microarray experiments and data analysis, and illustrate the use of small-angle X-ray scattering, nuclear magnetic resonance (NMR), and molecular modeling to study the structural biology of membrane transporters. Methods for exploring structure-function correlation, such as site-directed mutagenesis, immunocytochemistry, and confocal microscopy, are also described, along with several methods that may help in the development of novel therapeutics. Each technique contains hands-on instructions, many notes on avoiding the problems that one may encounter, and suggestions for variant procedures. Comprehensive and highly practical, Membrane Transporters: Methods and Protocols offers investigators working in biopharmaceutical science, drug discovery, and drug delivery both an array of powerful tools for work on membrane transporters and an essential guide to the state-of-the-art in the membrane transporter field today Bibliography Includes bibliographical references and index Notes English Print version record Subject Carrier proteins -- Laboratory manuals Membrane proteins -- Laboratory manuals Biological transport, Active -- Laboratory manuals Ion channels -- Laboratory manuals Pharmacogenetics. Membrane Transport Proteins Pharmacogenetics SCIENCE -- Life Sciences -- Cell Biology. Pharmacogenetics Biological transport, Active Carrier proteins Ion channels Membrane proteins Membranen. Biologisch transport. Wetenschappelijke technieken. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Yan, Qing, 1971- ISBN 1592593879 9781592593873 1588291049 9781588291042 1280842636 9781280842634 9786610842636 6610842639

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