Title Combinatorial peptide library protocols / edited by Shmuel Cabilly

Published Totowa, N.J. : Humana Press, ©1998

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Description 1 online resource (xiii, 313 pages) : illustrations (some color) Series Methods in molecular biology ; v. 87 Methods in molecular biology (Clifton, N.J.) ; v. 87 Contents Synthesis of a one-bead one-compound combinatorial peptide library / Kit S. Lam, Michal Lebl -- Enzyme-linked calorimetric screening of a one-bead one-compound combinatorial library / Kit S. Lam -- Synthesis and screening of positional scanning combinatorial libraries / Colette T. Dooley, Richard A. Houghten -- Synthesis and screening of peptide libraries on continuous cellulose membrane supports / Achim Kramer, Jens Schneider-Mergener -- Peralkylation : "libraries from libraries" : chemical transformation of synthetic combinatorial libraries / John M. Ostresh, Barbara Dörner, Richard A. Houghten -- Introduction to combinatorial solid-phase assays for enzyme activity and inhibition / Morten Meldal -- Preparation of biocompatible resins for library syntheses / Morten Meldal -- Intramolecular fluorescence-quenched substrate libraries / Morten Meldal -- The solid-phase enzyme inhibitor library assay / Morten Meldal -- Determination of peptide substrate motifs for protein kinases using a "one-bead one-compound" combinatorial library approach / Kit S. Lam -- The use of peptide library for the determination of kinase peptide substrates / Zhou Songyang, Lewis C. Cantley -- Analysis of protein kinase substrate specificity by the use of peptide libraries on cellulose paper (SPOT-Method) / Werner J. Tegge, Ronald Frank -- Generation of multiuse peptide libraries for functional screenings / Channa K. Jayawickreme, Shiranthi P. Jayawickreme, Michael R. Lerner -- Functional screening of multiuse peptide libraries using melanophore bioassay / Channa K. Jayawickreme, Shiranthi P. Jayawickreme, Michael R. Lerner -- The basic structure of filamentous phage and its use in the display of combinatorial peptide libraries / Shmuel Cabilly -- Construction and use of a 20-mer phage display epitope library / Baruch Stern, Jonathan M. Gershoni -- Construction of disulfide-constrained random peptide libraries displayed on phage coat protein VIII / Alessandra Luzzago, Franco Felici -- Conformational mimicry through random constraints plus affinity selection / Guangming Zhong -- The use of combinatorial libraries to identify ligands that interact with surface receptors in living cells / Shmuel Cabilly, Judith Heldman, Eliahu Heldman, Ephraim Katchalski-Katzir -- Screening phage display peptide libraries on nitrocellulose membranes / Shmuel Cabilly, Judith Heidman, Ephraim Katchalski-Katzir -- Identification of disease-specific epitopes / Antonella Folgori, Alessandra Luzzago, Paolo Monaci, Alfredo Nicosia, Riccardo Cortese, Franco Felici -- Identification of peptide ligands for the antigen binding receptor expressed on human B-cell lymphomas / Markus F. Renschler, William J. Dower, Ronald Levy -- Major histocompatibility complex allele-specific peptide libraries and identification of T-cell mimotopes / Marc A. Gavin, Michael J. Bevan -- Phage display of peptide libraries on protein scaffolds / Henry B. Lowman -- Displaying libraries of conformationally constrained peptides on the surface of Escherichia coli as flagellin fusions / Zhijan Lu, Brian C. Tripp, John M. McCoy -- Combinatorial peptide library as an immunogen / J. Estaquier, J.-C. Ameisen, C. Auriault, C. Boutillon, H. Gras-Masse, A. Tartar Summary Shmuel Cabilly presents in Combinatorial Peptide Library Protocols a collection of new and unique techniques for the construction and use of peptide libraries. These powerful methods-often detailed here by their pioneers-include protocols for the chemical synthesis of peptide libraries, for constructing peptide libraries that are displayed on the surface of filamentous phage or bacteria, and for the rapid screening of these libraries for molecules with biospecific properties. Additional methods permit identifying specific enzyme substrates, investigating the recognition spectra of various binding proteins, epitope mapping, and identifying mimotopes. Combinatorial Peptide Library Protocols offers novice and experienced investigators alike the ability to select molecules from a randomized pool having specific biological activities. Its state-of-the-art techniques, combined with clear step-by-step instructions, make this book an essential tool in the selection of peptides suitable for drug development Analysis peptiden peptides dna libraries eiwitten proteins genexpressie gene expression biologische technieken biological techniques protocollen protocols Molecular Biology (General) Moleculaire biologie (algemeen) Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Peptides -- Synthesis. Combinatorial chemistry. Protein binding. Carrier proteins. Peptide Library Peptides -- chemical synthesis Molecular Sequence Data Carrier Proteins Protein Binding Carrier proteins Combinatorial chemistry Peptides -- Synthesis Protein binding Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Cabilly, Shmuel. ISBN 9781592595716 1592595715 1280836873 9781280836879 9786610836871 6610836876

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