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Title Membrane transport : a practical approach / edited by Stephen A. Baldwin
Published Oxford ; New York : Oxford University Press, 2000


Description 1 online resource (xxi, 320 pages) : illustrations
Series The practical approach series ; 230
Practical approach series ; 230.
Contents Assay of membrane transport in cells and membrane vesicles / Simon M. Jarvis -- Reconstitution of membrane proteins : the Ca²-ATPase of sarcoplasmic reticulum / Anthony G. Lee -- The Xenopus oocyte expression system for the cDNA cloning and characterization of plasma membrane transport proteins / Sylvia Y.M. Yao, Carol E. Cass, and James D. Young -- Expression of foreign transport proteins in yeast / N. Sauer and J. Stolz -- Baculovirus-mediated overexpression of transport proteins / Gary J. Litherland and Stephen A. Baldwin -- The amplified expression, identification, purification, assay, and properties of hexahistidine-tagged bacterial membrane transport proteins / Alison Ward [and others] -- Spectroscopic and kinetic approaches for probing the mechanisms of solute transporters / Adrian R. Walmsley -- Detection and analysis of glucose transporters using photolabelling techniques / Alison K. Gillingham [and others] -- Computer prediction of transporter topology and structure / Rong-I Hong and Mark S.P. Sansom -- Two-dimensional crystallization of membrane proteins / Philippe Ringler, Bernard Heymann, and Andreas Engel -- Crystallization of membrane proteins / Tina D. Howard, Katherine E. McAuley-Hecht, and Richard J. Cogdell
Summary Membrane Transport contains a collection of experimental protocols for the study of proteins responsible for the transport of small molecules across biological membranes. It includes information on the latest methods for production of transport proteins by recombinant DNA technology, and for their investigation by techniques accessible to any well-found laboratory
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Carrier proteins -- Laboratory manuals
Biological transport -- Laboratory manuals
Cell membranes -- Experiments
Carrier proteins -- Experiments
Cytology -- Technique.
Carrier Proteins -- physiology
Cell Membrane -- physiology
Cells -- metabolism
Cytological Techniques
Protein Transport -- physiology
SCIENCE -- Life Sciences -- Biochemistry.
Cytology -- Technique.
Biological transport.
Carrier proteins.
Membranas celulares.
Transporte através da membrana.
Genre/Form Laboratory manuals.
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Baldwin, Stephen A
ISBN 9780191565823