Title Microbial carotenoids from bacteria and microalgae : methods and protocols / edited by Jose-Luis Barredo

Published New York : Humana Press : Springer, ©2012

Click on the following:
Springer eBooks

Copies

Description 1 online resource (xi, 355 pages) : illustrations Series Methods in molecular biology ; v. 892 Methods in molecular biology (Clifton, N.J.) ; v. 892 Contents Pathways of carotenoid biosynthesis in bacteria and microalgae / J. Paniagua-Michel, Jorge Olmos-Soto, and Manuel Acosta Ruiz -- Selection and taxonomic identification of carotenoid-producing marine actinomycetes / Francisco Romero -- Isolation, characterization, and diversity of novel radiotolerant carotenoid-producing bacteria / Dalal Asker [and others] -- Novel radio-tolerant astaxanthin-producing bacterium reveals a new astaxanthin derivative : astaxanthin dirhamnoside / Dalal Asker [and others] -- Novel zeaxanthin-producing bacteria isolated from a radioactive hot spring water / Dalal Asker [and others] -- Novel approach in the biosynthesis of functional carotenoids in Escherichia coli / Hisashi Harada and Norihiko Misawa -- Engineering Escherichia coli for canthaxanthin and ataxanthin biosynthesis / Qiong Cheng and Luan Tao -- Analysis of canthaxanthin production by Gordonia jacobaea / Patricia Veiga-Crespo [and others] -- Isolation and light-stimulated expression of canthaxanthin and spirilloxanthin biosynthesis genes from the photosynthetic bacterium Bradyrhizobium sp. Strain ORS278 / Eric Giraud and Andre Vermeglio -- Construction of carotenoid biosynthetic pathways through chromosomal integration in methane-utilizing bacterium Methylomonas sp. strain 16a / Rick W. Ye and Kristen Kelly -- Genetic modification in Bacillus subtilis for production of C30 carotenoids / Isamu Maeda -- Carotenoids' production from halophilic bacteria / Maria de Lourdes Moreno [and others] -- Construction and utilization of carotenoid reporter systems : identification of chromosomal integration sites that support suitable expression of biosynthetic genes and pathways / Pamela L. Sharpe and Deana J. DiCosimo -- Directed evolution of carotenoid synthases for the production of unnatural carotenoids / Maiko Furubayashi and Daisuke Umeno -- High-Throughput Screen for the Identi fi cation of improved catalytic activity: b-carotene hydroxylase / Mark A. Scaife [and others] -- DNA fingerprinting intron-sizing method to accomplish a specific, rapid, and sensitive identification of carotenogenic Dunaliella species / Jorge Olmos-Soto [and others] -- Ketocarotenoid biosynthesis in transgenic microalgae expressing a foreign beta-C-4-carotene oxygenase gene / Marta Vila [and others] -- Characterization of carotenogenesis genes in the cyanobacterium Anabaena sp. PCC 7120 / Hajime Masukawa, Mari Mochimaru, and Shinichi Takaichi -- Obtaining lutein-rich extract from microalgal biomass at preparative scale / Jose M. Fernandez-Sevilla, F. Gabriel Acien Fernandez, and Emilio Molina Grima -- NMR-based isotopologue profiling of microbial carotenoids / Eva Eylert, Adelbert Bacher, and Wolfgang Eisenreich -- Analysis of diapocarotenoids found in pigmented Bacillus species / Laura Perez-Fons and Paul D. Fraser Summary Carotenoids are a family of yellow to orange-red terpenoid pigments synthesized by photosynthetic organisms and many bacteria and fungi. They have beneficial health effects protecting against oxidative damage and may be responsible for the colours associated with plants and animals. In Microbial Carotenoids from Bacteria and Microalgae: Methods and Protocols, expert researchers in the field detail many of the most up-to-date methods which are now commonly used to study carotenoids. These include methods for the study of canthaxanthin production, construction of carotenoid reporter systems, directed evolution of carotenoid synthases, and improvement of b-carotene hydroxylase catalytic activity are described. Additionally, the book includes methods of DNA fingerprinting for the identification of carotenogenic Dunaliella species, ketocarotenoid biosynthesis in microalgae expressing the beta-C-4-carotene oxygenase gene, characterization of carotenogenesis genes in Anabaena sp., obtaining lutein from microalgal biomass, NMR-based isotopologue profiling of microbial carotenoids, and analysis of diapocarotenoids. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Carotenoids from Bacteria and Microalgae: Methods and Protocols provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms Analysis carotenoids bacteria algen algae microorganisms productie production Algae Bacteria Algen Bibliography Includes bibliographical references and index Notes English Print version record Subject Carotenoids -- Laboratory manuals Carotenoids -- biosynthesis Bacteria -- chemistry Microalgae -- chemistry Carotenoids Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Barredo, Jose-Luis. ISBN 9781617798795 1617798797

  Permalink