Title Cancer susceptibility : methods and protocols / volume editor, Michelle Webb

Published Totowa, N.J. : Humana ; London : Springer [distributor], 2010

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Description 1 online resource Series Methods in molecular biology ; 653 Methods in molecular biology (Clifton, N.J.) ; v. 653. 1064-3745 Contents The identification of colon cancer susceptibility genes by using genome-wide scans -- Prioritizing candidate genetic modifiers of BRCA1 and BRCA2 using a combinatorial analysis of global expression and polymorphism association studies of breast cancer -- Microarray-based comparative genomic hybridization (array-CGH) as a useful tool for identifying genes involved in Glioblastoma (GB) -- Multiplex Amplifiable Probe Hybridization (MAPH) methodology as an alternative to comparative genomic hybridization (CGH) -- Utilizing Saccharomyces cerevisiae to identify aneuploidy and cancer susceptibility genes -- Computational identification of cancer susceptibility loci -- Digital candidate gene approach (DigiCGA) for identification of cancer genes -- The use of denaturing high performance liquid chromatography (DHPLC) for mutation scanning of hereditary cancer genes -- Enhanced mismatch mutation analysis: simultaneous detection of point mutations and large scale rearrangements by capillary electrophoresis, application to BRCA1 and BRCA2 -- Economical protocol for combined single-strand conformation polymorphism and heteroduplex analysis on a standard capillary electrophoresis apparatus -- Mutational screening of hMLH1 and hMSH2 that confer inherited colorectal cancer susceptibility using denature gradient gel electrophoresis (DGGE) -- s-RT-MELT: a novel technology for mutation screening -- Zoom-in array comparative genomic hybridization (aCGH) to detect germline rearrangements in cancer susceptibility genes -- Development of a scoring system to screen for BRCA1/2 mutations -- Use of splicing reporter minigene assay to evaluate the effect on splicing of unclassified genetic variants -- Functional analysis of human BRCA2 variants using a mouse embryonic stem cell-based assay -- Developing functional assays for BRCA1 unclassified variants Summary Over the past two decades, spectacular advances have been made in our understanding of the molecular genetics of cancer, leading to the pursuit of identifying genes that, when mutated, result in an increased susceptibility to the disease. In Cancer Susceptibility: Methods and Protocols, experts in the field bring together the most recent technological developments for identifying and screening cancer susceptibility genes. Divided into two clear sections, the book begins with gene identification, which updates and informs scientists working at identifying novel cancer susceptibility genes, while the second part deals with mutation screening technologies that aid scientists and clinicians working to translate this knowledge into the clinic. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Susceptibility: Methods and Protocols is a timely collection that seeks to provide researchers with the tools to predict and combat this terrible disease Analysis neoplasms biomedische wetenschappen biomedicine humane genetica human genetics Medicine (General) Geneeskunde (algemeen) Bibliography Includes bibliographical references and index Notes English Print version record Subject Cancer -- Susceptibility. Cancer -- Genetic aspects. Diagnostic services. Genetics -- Technique. Disease susceptibility. Biology. Genetic screening. Methodology. Diagnosis. Life sciences. National health services. Physical sciences. Medical care. Diseases. Genetic Services Diagnostic Techniques and Procedures Diagnostic Services Publication Formats Investigative Techniques Genetic Techniques Genotype Disease Susceptibility Biology Genetic Predisposition to Disease Laboratory Manuals Genetic Testing Methods Genetics Analytical, Diagnostic and Therapeutic Techniques and Equipment Disease Attributes Diagnosis Publication Characteristics Biological Science Disciplines Health Services Preventive Health Services Genetic Phenomena Natural Science Disciplines Phenomena and Processes Community Health Services Health Care Facilities, Manpower, and Services Pathologic Processes Pathological Conditions, Signs and Symptoms Delivery of Health Care Disciplines and Occupations Disease State Medicine Patient Care biology. methodology. diagnosis. biological sciences. physical sciences. illness. disease. Physical sciences National health services Methodology Medical care Life sciences Genetics -- Technique Genetic screening Diseases Disease susceptibility Diagnostic services Diagnosis Biology Cancer -- Genetic aspects Cancer -- Susceptibility Medicine. Oncology. Health & Biological Sciences. Form Electronic book Author Webb, Michelle. ISBN 9781607617594 1607617595

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