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Book Cover
E-book

Title Barley : methods and protocols / edited by Wendy A. Harwood
Published New York : Humana Press : Springer, [2019]
©2019

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Description 1 online resource (xi, 313 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 1900
Methods in molecular biology (Clifton, N.J.) ; v. 1900. 1064-3745
Contents Introduction to barley : the crop and the model / Wendy A. Harwood -- Mutation breeding in barley : historical overview / Ludmila Ohnoutkova -- Practical guide to barley crossing / William T.B. Thomas, Hazel Bull, Allan Booth, Ruth Hamilton, Brian P. Forster, and Jerome D. Franckowiak -- Barley anther culture / Ludmila Ohnoutkova, Tomas Vlcko, and Mentewab Ayalew -- Isolated microspore culture in barley / Patricio Esteves and Francois J. Belzile -- TILLING in barley / Matthias Jost, Miriam Szurman-Zubrzycka, Katarzyna Gajek, Iwona Szarejko, and Nils Stein -- Virus-induced gene silencing (VIGS) for functional characterization of disease resistance genes in barley seedlings / Lokanadha R. Gunupuru, Alexandre Perochon, Shahin S. Ali, Steven R. Scofield, and Fiona M. Doohan -- Agrobacterium-mediated transformation of barley immature embryos / Alison Hinchliffe and Wendy A. Harwood -- Methods for the simple and reliable assessment of barley sensitivity to abiotic stresses during early development / Agata Daszkowska-Golec, Anna Skubacz, Marzena Kurowska, Micha℗ S℗ota, Dorota Swiergolik, and Iwona Szarejko -- Preparation of barley roots for histological, structural, and immunolocalization studies using light and electron microscopy / Marek Marzec and Michael Melzer -- Preparation of barley pollen mother cells for confocal and super resolution microscopy / Sybille Mittmann, Mikel Arrieta, Luke Ramsay, Robbie Waugh, and Isabelle Colas -- Microarrays for high-throughput gene expression analysis of barley / Jenny A. Morris and Pete E. Hedley -- Genome engineering using TALENs / Goetz Hensel and Jochen Kumlehn -- Creating targeted gene knockouts in barley using CRISPR/Cas9 / Tom Lawrenson and Wendy A. Harwood -- Genotyping-by-sequencing on the ion torrent platform in barley / Amina Abed, Gaetan Legare, Sonia Pomerleau, Jerome St-Cyr, Brian Boyle, and Francois J. Belzile -- DNA methylation analysis in barley and other species with large genomes / Karolina Chwialkowska, Urszula Korotko, and Miroslaw Kwasniewski -- High-resolution RT-PCR analysis of alternative barley transcripts / Craig G. Simpson, John Fuller, Paulo Rapazote-Flores, Claus-Dieter Mayer, Cristiane P.G. Calixto, Linda Milne, Pete E. Hedley, Clare Booth, Robbie Waugh, and John W.S. Brown -- Exome capture for variant discovery and analysis in barley / Micha Bayer, Jenny A. Morris, Clare Booth, Allan Booth, Niki Uzrek, Joanne R. Russell, Robbie Waugh, and Pete E. Hedley
Bibliography Includes bibliographical references and index
Notes Online resource; title form PDF title page (SpringerLink, viewed November 20, 2018)
Subject Hordeum.
Hordeum
Agricultural science.
Plant reproduction & propagation.
Technology & Engineering -- Agriculture -- General.
Science -- Life Sciences -- Botany.
Hordeum
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Harwood, Wendy A., editor
ISBN 9781493989447
1493989448
9781493989423
1493989421