Description |
1 online resource (387 pages) |
Contents |
Front Cover; Origins of Clinical Chemistry: The Evolution ofProtein Analysis; Copyright Page; Dedication; Table of Contents; Preface; Acknowledgments; Introduction; Chapter 1. Protein: Concept and Controversy; I. Protein: The Ubiquitous Molecule; II. Metabolism of Protein; III. The Development of Protein Chemistry; IV. The Word and the Concept; V. The Protein Theory of Mulder; VI. Liebig's Analyses; VII. Mulder's Conflict with Liebig; VIII. Liebig's Laboratory at Giessen; IX. Dumas; Chapter 2. The Colloidal State; I. The Tyndall Effect; II. The Ultramicroscope; III. Different Worlds of Matter |
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IV. The Size of Colloidal ParticlesChapter 3. The Origins of Organic Chemistry; I. The Vital Force; II. The Chemical Nature of Organic Compounds; III. The Discovery of Oxygen, Nitrogen, and Hydrogen; IV. Lavoisier; V. The Diversity of Organic Compounds; VI. Wöhler's Synthesis of Urea; VII. The New Organic Chemistry; VIII. Discovery of the Amino Acids; IX. Chemical Techniques for Separating Amino Acids; X. The Vast Diversity of Protein Structure; XI. Protein Structure: The Fischer-Hofmeister Theory; XII. Molecular Size of Proteins; Chapter 4. The Kjeldahl Method for Nitrogen; I. Introduction |
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II. The Dumas Method for NitrogenIII. The Carlsberg Laboratory; IV. Development of the Kjeldahl Method; V. Impact of the Kjeldahl Method; VI. Modifications and Improvements; VII. Application of the Method to Combined Nitrogen; VIII. Description of the Procedure; IX. Current Status; X. Kjeldahl Analysis of Plasma Proteins; Chapter 5. Classification of Proteins; I. Introduction; II. Simple Proteins; III. Conjugated Proteins; IV. Derived Proteins; V. The Basis of Plasma Protein Analysis; Chapter 6. Detection of Protein; I. Introduction; II. Criteria of Purity; III. Analysis of Protein |
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Chapter 7. Protein FractionationI. Introduction; II. Early Distinctions between Albumin and Globulin; III. The Precipitating Action of Ammonium Sulfate; IV. The Precipitating Action of Sodium Sulfate; V. Howe's Method for Fractionating Serum Proteins; VI. Advantages and Disadvantages of Howe's Method; VII. Inhomogeneity of Salt-Precipitated Fractions; VIII. Discrepancy between Electrophoresis and Salt Precipitation Methods; IX. Modification and Improvement of Howe's Method; X. Precipitation of Globulin with Sodium Sulfite; XI. Protein Precipitation with Phosphate Buffers |
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XII. Precipitating Action of Heavy Metals and Alkaloidal ReagentsXIII. Precipitation with Organic Solvents; Chapter 8. Nonspecific Tests and Procedures; I. Determination of Physical Properties of Protein Solutions; II. The Turbidity Procedures; Chapter 9. Colorimetry and Photometry; I. Introduction; II. Colorimetry; III. Nephelometry; IV. Turbidimetry; V. Photometric Colorimetry; VI. The Biuret Reaction; VII. Phenol Reaction for Tyrosine; VIII. Ultraviolet Absorbance (250-300 nm); IX. Far Ultraviolet Absorbance (200-250 nm); X. Biuret: The Popular Choice; XI. Albumin-Specific Dye Binding |
Summary |
Origins of Clinical Chemistry |
Notes |
XII. Direct Colorimetric Analysis of Globulin |
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Print version record |
Subject |
Blood proteins -- Analysis -- History
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Clinical chemistry -- History
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MEDICAL -- Physiology.
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SCIENCE -- Life Sciences -- Human Anatomy & Physiology.
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Blood proteins -- Analysis
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Clinical chemistry
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Genre/Form |
History
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Form |
Electronic book
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ISBN |
9780323152921 |
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0323152929 |
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