| Description |
1 online resource (xiv, 517 pages) : illustrations |
| Series |
Methods in molecular medicine, 1543-1894 ; 120 |
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Methods in molecular medicine ; 120. 1543-1894
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| Contents |
Breast tissue banking: collection, handling, storage, and release of tissue for breast cancer research / Linda Snell and Peter H. Watson -- Frozen biopsy collection and storage / Russell D. Leek -- Serial biopsies/FNAs and their assessment / Irene Boeddinghaus and Stephen R.D. Johnson -- Breast tissue microarrays / Robert J. Springall and Cheryl E. Gillett -- Preparation of tumor homogenates for subsequent preparation of cytosols, membrane fractions, RNA, and DNA / Naomi Robertson and Russell Leek -- Isolation of RNA from tumor samples: single-step guanidinium acid/phenol method / Naomi Robertson and Russell Leek -- Isolation of DNA from tumor samples / Naomi Robertson and Russell Leek -- Laser-assisted microdissection and isolation of DNA and RNA / Ulrich Lehmann and Hans Kreipe |
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Traditional and established indicators of prognosis and treatment success / Derek E. Roskell and Ian D. Buley -- Sentinel lymph node biopsy in breast cancer / Celia Chao and Kelly M. McMasters -- Pathological evaluation of axillary sentinel lymph nodes in breast cancer / Michael Z. Gilcrease and Aysegul Sahin -- Measurement of estrogen receptor status by immunocytochemistry in paraffin wax sections / Bharat Jasani [and others] -- Markers of apoptosis / Falzul H. Sarkar and Yiwei Li -- Quantitative angiogenesis in breast cancer / Stephen B. Fox |
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Immunohistochemistry / Cheryl E. Gillett -- Detection of aberrant glycosylation in breast cancer using lectin histochemistry / Tracey M. Carter and Susan A. Brooks -- SDS-PAGE and western blotting to detect proteins and glycoproteins of interest in breast cancer research / Chloe Osborne and Susan A. Brooks -- Breast cancer proteomics using two-dimensional electrophoresis / Miriam V. Dwek and Sarah L. Rawlings -- Procedures for the quantitative protein determination of urokinase and its inhibitor PAI-1 in human breast cancer tissue extracts using ELISA / Manfred Schmitt [and others] |
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Fluorescence in situ hybridization and comparative genomic hybridization / Patricia Gorman and Rebecca Roylance -- Fluorescence in situ hybridization assessment of c-myc amplification in breast cancer tissues / Jan K. Blancato, Mary Steele Williams, and Robert B. Dickson -- Detection of HER2 gene amplification by FISH in breast cancer / John M.S. Bartlett, and Amanda Forsyth -- In situ hybridization combined with immunohistochemistry to localize gene expression / Rosemary Jeffery, Toby Hunt, and Richard Poulsom -- Quantitation of RNA by ribonuclease protection assay / John W. Moore -- Identification of steroid hormone-regulated genes in breast cancer / Bruce R. Westley and Felicity E.B. May -- Sequencing of the tumor suppressor gene TP 53 / Barbro Linderholm, Torbjorn Norberg, and Jonas Bergh -- Expression profiling using cDNA microarrays / Chris Jones [and others] -- Gene expression analysis using filter cDNA microarrays / Peter Simpson [and others] |
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Methods to analyze the effects of the urokinase system on cancer cell adhesion, proliferation, migration, and signal transduction events / Ute Reuning [and others] -- Phosphospecific antibodies as a tool to study in vivo regulation of BRCA1 after DNA damage / Kum Kum Khanna, Magtouf Gatei, and Gordon Tribbick -- Models of hormone resistance in vitro and in vivo / Jennifer MacGregor Schafer and V. Craig Jordan -- Generation of genetically modified embryonic stem cells for the development of knockout mouse animal model systems / Stephen D. Robinson, Stephen Wilson, and Kairbaan M. Hodivala-Dilke -- In vivo xenograft models of breast cancer metastatsis / Ursula Valentiner, Susan A. Brooks, and Udo Schumacher -- Neoadjuvant endocrine therapy models / Juliette Murray, William R. Miller, and J. Michael Dixon -- Primary mouse endothelial cell culture for assays of angiogenesis / Louise E. Reynolds and Kairbaan M. Hodivala-Dilke |
| Summary |
Many new technologies involving large-scale analysis of gene expression, novel treatments, and emergent combined therapy approaches have opened fresh possibilities for understanding and effectively managing breast cancer. In Breast Cancer Research Protocols, active researchers and clinicians describe in detail both well-established and cutting-edge methods for investigating breast cancer biology not only in the laboratory, but also in clinical settings. These readily reproducible techniques solve a variety of problems, ranging from how to collect, store, and prepare human breast tumor samples for analysis, to analyzing cells in vivo and in vitro. Additional chapters address the technology of handling biopsies, new methods for analyzing genes and gene expression, markers of clinical outcome and progress, analysis of tumor-derived proteins and antigens, validating targets, and investigating the biology of newly discovered genes. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Breast Cancer Research Protocols offers laboratory and clinical researchers the tools needed to illuminate breast cancer biology and advance the efficacy of its treatment today |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
| In |
Springer e-books |
| Subject |
Breast -- Cancer -- Laboratory manuals
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Genetics -- Technique.
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Breast Neoplasms -- genetics
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Breast Neoplasms -- pathology
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Biopsy -- methods
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Clinical Protocols -- methods
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Genetic Techniques
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MEDICAL -- Oncology.
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HEALTH & FITNESS -- Diseases -- Cancer.
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Breast -- Cancer
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| Genre/Form |
Handbooks and manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Brooks, Susan A
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|
Harris, Adrian, 1950-
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| ISBN |
158829191X |
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9781588291912 |
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1592599699 |
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9781592599691 |
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1280358726 |
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9781280358722 |
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9786610358724 |
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6610358729 |
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