Front Cover; Contents; Acknowledgment; About the Author ; Preface: How to Use This Manual; Introduction: Infrastructure and Safety Requirements; 1"Transformation of Competent Bacteria with a DNA Plasmid; 2"Isolation of Plasmids by Miniprep and Restriction Enzyme Analysis; 3"Designing a New Construct Using Restriction Enzymes (Computer Lab); 4"Restriction Enzymes; 5"Ligation; 6"Screening a Ligation and Choosing Recombinants; 7"Use of Software to Design Primers for Cloning Using the Polymerase Chain Reaction (Computer Lab); 8"Running the Polymerase Chain Reaction
9"Protein Expression and Purification10"Characterization of Purified Protein; 11"Protein Crystallization; 12"Transfecting Mammalian Cells with Custom-Designed Constructs; 13"Screening Fluorescent Reporter Gene Expression in Mammalian Cells; 14"Synthesis of Quantum Dots; 15"Synthesis of Gold Nanoparticles; 16"Electrophysiology: Lipid Bilayer Recording; 17"Electrophysiology Alternative: Introduction to Lipid Vesicles; Appendix 1"Disposables and Supplies Needed; Appendix 2"Instructors' Guide to Behind-the-Scenes Preparation; Appendix 3"Grading Recommendations and Guidelines
Summary
Molecular CloningTransformation of Competent Bacteria with a DNA Plasmid Isolation of Plasmids by Miniprep and Restriction Enzyme Analysis Designing a New Construct Using Restriction Enzymes (Computer Lab) Restriction Enzymes Ligation Screening a Ligation and Choosing Recombinants Use of Software to Design Primers for Cloning Using the Polymerase Chain Reaction (Computer Lab) Running the Polymerase Chain Reaction Gene ExpressionProtein Expression and Purification Characterization of Purified Protein Protein Crystallization Fluorescent Reporter Genes in Mammalian CellsTransfecting Mammalian Cel
