| Description |
1 online resource (xi, 366 pages) : illustrations (some color) |
| Series |
Methods in Molecular Biology, 1940-6029 ; 1197 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1197. 1064-3745
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| Contents |
Galleria mellonella as an infection model for select agents / Nicolas Sprynski, Eric Valade, and Fabienne Neulat-Ripoll -- Drosophila as a model for intestinal infections / Matthieu Lestradet, Kwang-Zin Lee, and Dominique Ferrandon -- Zebrafish embryos as a model to study bacterial virulence / Jennifer Mesureur and Annette C. Vergunst -- Studying host-pathogen interaction events in living mice visualized in real time using biophotonic imaging / Gary Splitter [and others] -- Intravital two-photon imaging to understand bacterial infections of the mammalian host / Ferdinand X. Choong and Agneta Richter-Dahlfors -- Cre reporter assay for translocation (CRAfT) : a tool for the study of protein translocation into host cells / Amke den Dulk-Ras, Annette C. Vergunst, and Paul J.J. Hooykaas -- Detection of the interaction between host and bacterial proteins : eukaryotic nucleolin interacts with Francisella elongation factor tu / Monique Barel and Alain Charbit -- Hijacking the host proteasome for the temporal degradation of bacterial effectors / Tomoko Kubori, Andree M. Hubber, and Hiroki Nagai -- Live cell imaging of phosphoinositide dynamics during Legionella infection / Stephen Weber and Hubert Hilbi -- Investigating interference with apoptosis induction by bacterial proteins / Hua Niu and Yasuko Rikihisa -- Bimolecular fluorescence complementation for imaging protein interactions in plant hosts of microbial pathogens / Lan-Ying Lee and Stanton B. Gelvin -- Investigating TLR signaling responses in murine dendritic cells upon bacterial infection / Suzana Pinto Salcedo and Lena Alexopoulou -- siRNA screens using Drosophila cells to identify host factors required for infection / Aseem Pandey [and others] -- Purification of intracellular bacteria : isolation of viable Brucella abortus from host cells / Esteban Chaves-Olarte [and others] -- RNA sequencing of FACS-sorted immune cell populations from zebrafish infection models to identify cell specific responses to intracellular pathogens / Julien Rougeot [and others] -- Taking the shortcut for high-throughput shotgun proteomic analysis of bacteria / Erica Marie Hartmann [and others] -- Comparative genomic analysis at the PATRIC, a bioinformatic resource center / Alice R. Wattam [and others] -- Markerless deletion method for genetic manipulation of Burkholderia cenocepacia and other multidrug-resistant gram-negative bacteria / Daniel F. Aubert, Mohamad A. Hamad, and Miguel A. Valvano -- Gene inactivation in Coxiella burnetii / Paul A. Beare and Robert A. Heinzen -- Chemical mutagenesis approach to identify virulence determinants in the obligate intracellular pathogen Chlamydia trachomatis / Bidong Nguyen and Raphael Valdivia |
| Summary |
Annotation Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics. Chapters focus on techniques that can be used to investigate different aspects of the physiopathology of bacterial infections, from the whole animal to tissue, cellular and molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Host-Bacteria Interactions: Methods and Protocols provide researchers with a comprehensive account of the practical steps necessary for carrying out each protocol successfully |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed September 12, 2014) |
| Subject |
Host-bacteria relationships -- Laboratory manuals
|
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Bacteria.
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Schizomycetes.
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Communicable diseases.
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Biological models.
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Bacteria
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Communicable Diseases
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Host-Pathogen Interactions
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Bacterial Infections -- physiopathology
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Bacteriological Techniques -- methods
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Models, Biological
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Schizomycetes
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Communicable diseases
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Biological models
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Bacteria
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Host-bacteria relationships
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Vergunst, Annette C., 1966- editor.
|
|
O'Callaghan, David, Prof., editor.
|
| LC no. |
2014945547 |
| ISBN |
9781493912612 |
|
1493912615 |
|
1493912607 |
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9781493912605 |
|
