Title Autophagosome and phagosome / edited by Vojo Deretic

Published Totowa, N.J. : Humana Press, ©2008

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Description 1 online resource (xvii, 455 pages) : illustrations Series Methods in molecular biology ; v. 445 Methods in molecular biology (Clifton, N.J.) ; v. 445 Contents Autophagosome and phagosome / V. Deretic -- Fine structure of the autophagosome / E.L. Eskelinen -- Methods for assessing autophagy and autophagic cell death / E. Tasdemir [and others] -- LC3 and autophagy / I. Tanida, T. Ueno and E. Kominami -- Amino acid regulation of autophagosome formation / A.J. Meijer -- Autophagic proteolysis of long-lived proteins in nonliver cells / E.A. Roberts and V. Deretic -- Autophagosomes in GFP-LC3 transgenic mice / N. Mizushima and A. Kuma -- Experimental control and characterization of autophagy in Drosophila / G. Juhasz and T.P. Neufeld -- Analysis of autophagosome membrane cycling by fluorescence microscopy / J.E. Legakis and D.J. Klionsky -- Protein trafficking into autophagosomes / A. Young and S. Tooze -- Sphingolipids in macroautophagy / G. Lavieu [and others] -- Molecular links between autophagy and apoptosis / I.A. Ciechomska, C.G. Goemans and A.M. Tolkovsky -- Clearance of mutant aggregate-prone proteins by autophagy / B. Ravikumar, S. Sarkar and D.C. Rubinsztein -- Localization and MHC class II presentation of antigens targeted for macroautophagy / D. Schmid and C. Munz -- Chaperone-mediated autophagy / S. Kaushik and A.M. Cuervo Microautophagy in the yeast Saccharomyces cerevisiae / A. Uttenweiler and A. Mayer -- EM analysis of phagosomes / C. de Chastellier -- Analysis of phosphoinositide dynamics during phagocytosis using genetically encoded fluorescent biosensors / G. Cosio and S. Grinstein -- In vitro phagosome-endosome fusion / I. Vergne and V. Deretic -- Real-time spectrofluorometric assays for the lumenal environment of the maturing phagosome / R.M. Yates and D.G. Russell -- Maturation changes in purified phagosomes / R. Dieckmann [and others] -- Large-scale purification of latex bead phagosomes from mouse macrophage cell lines and subsequent preparation for high-throughput quantitative proteomics / A. Rupper and J. Cardelli -- Class II MHC antigen processing in phagosomes / L. Ramachandra, W.H. Boom and C.V. Harding -- Analyzing association of the endoplasmic Reticulum with the Legionella pneumophila-Containing Vacuoles by Fluorescence Microscopy / A. Ingmundson and C.R. Roy -- fractionation of the Coxiella burnetii parasitophorous vacuole / D. Howe and R.A. Heinzen -- Bacterial phagosome acidification within IFN-gamma-activated macrophages : role of host p47 immunity-related GTPases (IRGs) / S. Tiwari and J.D. Macmicking -- SopE-mediated recruitment of host Rab5 on phagosomes inhibits Salmonella transport to lysosomes / R. Madan, G. Krishnamurthy and A. Mukhopadhyay -- The Mycobacterium tuberculosis phagosome / E.A. Roberts and V. Deretic Summary Autophagy and phagocytosis are distinct yet partially morphologically similar processes. In Autophagosome and Phagosome, authoritative scientists present easy-to-follow methods on autophagy, a rapidly growing field with a need for standards of assessment, and phagocytosis, a relatively mature field which could benefit greatly from updated methods, in order to prompt further explorations of their similarities and differences. The methods on autophagy allow the reader to find appropriate techniques to identify, monitor, and quantify autophagic processes, while the methods devoted to phagocytosis provide researchers with several modern techniques for in vitro and in vivo studies of phagosomal organelles. Following the successful Methods in Molecular Biology series format, chapters include step-by-step laboratory protocols, lists of necessary materials, and tips for troubleshooting and avoiding known pitfalls. Comprehensive and forward-thinking, Autophagosome and Phagosome offers a valuable guide to both cellular processes while inciting researchers to explore the potentially important connections between the two Bibliography Includes bibliographical references and index Notes English Print version record Subject Endocytosis -- Research -- Methodology Autophagic vacuoles -- Research -- Methodology Phagocytosis -- Research -- Methodology Cell physiology -- Methodology Phagocytosis. Endocytosis. Natural immunity. Immune system. Cell physiology. Immunity. Autophagy Phagocytosis Endocytosis Immunity, Innate Blood Physiological Phenomena Cell Death Immune System Phenomena Cell Physiological Phenomena Immunity Circulatory and Respiratory Physiological Phenomena Phenomena and Processes Autophagic vacuoles Phagocytosis Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Deretic, Vojo. ISBN 9781597451574 1597451576

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