Title Asthma : mechanisms and protocols / edited by K. Fan Chung and Ian Adcock

Published Totowa, N.J. : Humana Press, ©2000

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Description 1 online resource (xi, 346 pages) : illustrations Series Methods in molecular medicine ; 44 Methods in molecular medicine ; 44. Contents Asthma: Application of cell and molecular biology techniques to unravel causes and pathophysiological mechanisms / F. Chung and I. Adcock-Culture of normal human airway epithelial cells and measurement of mucin synthesis and secretion / R. Wu-Brush biopsy and culture of airway epithelial cells: Beta-adrenergic receptor system function / S.G. Kelsen, D. Ciccolella and K. Brennan-Isolation and characterization of human airway fibroblasts in culture / J. Chakir [and others]-Flow cytometric analysis of blood monocytes and alveolar macrophages / M.L. Taylor and B.S. Bochner-Expression of il-10 and gm-csf in blood monocytes and alveolar macrophages / M. John and S. Lim-Map kinase expression in eosinophils / P.M. de Souza [and others]-Human eosinophil isolation and the measurement of apoptosis / H. Kankaanranta [and others]-Adhesion of t-cells to airway smooth muscle cells / A.L. Lazaar and R.A. Panettieri, Jr.-Proliferation of circulating t-cells / M. Larche-Differential display analysis of inflammatory genes in airway epithelium / L.M. Schwiebert-Analysis of transcription factor activation: Nfkappab as a regulator of inflammatory genes in epithelial cells / R. Newton and I.M. Adcock-Transient transgenic approaches for investigating the role of gm-csf in pulmonary inflammation and immune diseases / Z. Xing, M.R. Stampfli and J. Gauldie-Quantitative analysis of rantes mrna in endobronchial biopsies using polymerase chain reaction / N. Berkman-Intracellular cytokine staining for analysis by flow cytometry / A.J. Frew, J. Madden and P. Bakakos-Localization of il-4 and il-5 mrna in bronchial biopsies / Y. Nakamura, P. Christodoulopoulos and Q. Hamid-Immunohistochemical analysis of adhesion molecules in airway biopsies / J.W.S and T.H. S-Site-directed mutagenesis in the investigation of beta-adrenoreceptor exosite / S.A. Green-Methods for determining beta(2)-adrenoreceptor genotype / C.D. J, P.W.A and P.H. I-Modulation of beta-adrenoreceptor expression in the airways / C.W.M. J-Chemical mutational analysis of glucocorticoid receptor / J.L.S and H.L. T-Histone acetylation and deacetylation / K. Ito, J.B.P and M.A. I-Genome-wide search for asthma genes / H.M.A and F.J.M.J Summary As asthma increases in prevalence and severity around the world, the need for understanding its basic mechanisms and for developing better drugs and preventative measures becomes more urgent. In Asthma: Mechanisms and Protocols, K. Fan Chung and Ian Adcock bring together the first collection of laboratory methods for studying the molecular and cellular mechanisms of asthma. Leading experts describe highly effective methods for obtaining cells from asthmatic airways, analyzing gene and protein expression in these clinical samples, and using molecular and cellular tools to study cytokine expression and release in this disease. There are also cutting-edge techniques for studying asthma-related genes and genetic polymorphisms, and for understanding the effects of asthma treatment. User-friendly and state-of-the-art, Asthma: Mechanisms and Protocols provides all asthma researchers-whether novice or experienced, whether in experimental or clinical research-with a first-class collection of readily reproducible pharmacological, cellular, molecular, biochemical, and genetic methods for elucidating not only the mechanisms of the disease itself, but also of the drugs for asthma's treatment Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL In Springer Protocols Subject Asthma -- Molecular aspects -- Laboratory manuals Bronchi -- Diseases. Respiratory allergy. Proteins. Respiratory organs -- Diseases. Allergy. Lungs -- Diseases. Diseases. Immunologic diseases. Cytokines. Asthma. Medical laboratory technology. Biological products. Bronchial Diseases Respiratory Hypersensitivity Intercellular Signaling Peptides and Proteins Investigative Techniques Lung Diseases, Obstructive Peptides Proteins Respiratory Tract Diseases Analytical, Diagnostic and Therapeutic Techniques and Equipment Hypersensitivity, Immediate Lung Diseases Biological Factors Amino Acids, Peptides, and Proteins Disease Chemicals and Drugs Hypersensitivity Immune System Diseases Cytokines Asthma Clinical Laboratory Techniques protein. MEDICAL -- Pulmonary & Thoracic Medicine. HEALTH & FITNESS -- Diseases -- Respiratory. Respiratory organs -- Diseases Respiratory allergy Proteins Medical laboratory technology Lungs -- Diseases Immunologic diseases Diseases Cytokines Bronchi -- Diseases Asthma Allergy Asthma -- Molecular aspects Wetenschappelijke technieken. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Chung, K. Fan, 1951- Adcock, Ian ISBN 9781592590728 1592590721

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