| Description |
1 online resource (xv, 424 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 618 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 618
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| Contents |
Antimicrobial peptides in frog skin secretions / J.M. Conlon and A. Sonnevend -- Purification of antimicrobial peptides from human skin / J.M. Schroder -- Strategies for the isolation and characterization of antibacterial lantibiotics / D. Jabes and S. Donadio -- Expression and purification of recombinant alpha-defensins and alpha-defensin precursors in Escherichia coli / S. Figueredo [and others] -- Production of recombinant antimicrobial peptides in bacteria / M. Zorko and R. Jerala -- Methods for building quantitative structure-activity relationship (QSAR) descriptors and predictive models for computer-aided design of antimicrobial peptides / O. Taboureau -- Synthesis and thermodynamic characterization of small cyclic antimicrobial arginine and tryptophan-rich peptides with selectivity for Gram-negative bacteria / M. Bagheri -- Synthesis of antimicrobial peptides using the SPOT technique / D.F. Winkler and K. Hilpert -- High-throughput screening for antimicrobial peptides using the SPOT technique / K. Hilpert -- Antimicrobial peptides: the LPS connection / A. Giuliani, G. Pirri and A.C. Rinaldi -- Binding and permeabilization of model membranes by amphipathic peptides / P.F. Almeida and A. Pokorny -- Comparing bacterial membrane interactions of antimicrobial peptides and their mimics / N.P. Chongsiriwatana and A.E. Barron -- Dynamic transitions of membrane-active peptides / S.L. Grage, S. Afonin and A.S. Ulrich -- Solid-state NMR investigations of membrane-associated antimicrobial peptides / C. Aisenbrey, P. Bertani and B. Bechinger -- Use of atomic force microscopy as a tool to understand the action of antimicrobial peptides on bacteria / A. Li [and others] -- Fluorescence and electron microscopy methods for exploring antimicrobial peptides mode(s) of action / L. Marcellini [and others] -- Molecular simulations of antimicrobial peptides / A. Langham and Y.N. Kaznessis -- Computer-based analysis, visualization, and interpretation of antimicrobial peptide activities / R. Mikut -- Potential therapeutic application of host defense peptides / L. Zhang and T.J. Falla -- Therapeutic potential of HDPs as immunomodulatory agents / H. Jenssen and R.E. Hancock -- Assay systems for measurement of anti-inflammatory activity / E. Rubinchik and C. Pasetka -- Ex vivo skin infection model / E. Rubinchik and C. Pasetka -- Measuring antimicrobial peptide activity on epithelial surfaces in cell culture / G. Diamond [and others] -- Antimicrobial and antibiofilm activity of quorum sensing peptides and Peptide analogues against oral biofilm bacteria / K. LoVetri and S. Madhyastha -- Characterization of the leishmanicidal activity of antimicrobial peptides / J.R. Luque-Ortega and L. Rivas |
| Summary |
The action of antimicrobial peptides (AMPs), ranging from direct killing of invading pathogens to immune response modulation and other complex biological responses, has stimulated research and clinical interest for more than two decades, but the area is still burgeoning due to emerging discoveries in the functions, roles, and regulation of AMPs, thus making the study of antimicrobial peptides a multi-disciplinary and rapidly evolving field. In Antimicrobial Peptides: Methods and Protocols, leading investigators present a broad, up-to-date collection of current research and experimental methods for the isolation, characterization, production, and optimization of antimicrobial peptides. Additional chapters detail methodologies in several microscopy techniques, high-throughput screening, QSAR modeling, and computer-aided design used to study these compounds, while key review articles survey potential medical applications of antimicrobial peptides as innovative anti-infective and immunomodulatory agents, as well as emerging discoveries in their function, regulation, and roles in innate immunity. As a volume in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and wide-ranging in its applications, Antimicrobial Peptides: Methods and Protocols provides both an authoritative guide for lab work on AMPs or related substances and a useful collection of thought stimuli to inspire further scientific endeavours in a wide array of vital fields |
| Analysis |
immunologie |
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immunology |
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antilichamen |
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antibodies |
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biomedische wetenschappen |
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biomedicine |
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Medicine (General) |
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Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references (pages 418-420) and index |
| Notes |
English |
|
Print version record |
| Subject |
Peptide antibiotics.
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Microbial pesticides.
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Anti-infective agents.
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Natural immunity.
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Antimicrobial Cationic Peptides -- therapeutic use
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Anti-Infective Agents
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Immunity, Innate
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Natural immunity
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Anti-infective agents
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Microbial pesticides
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Peptide antibiotics
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| Genre/Form |
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Giuliani, Andrea.
|
|
Rinaldi, Andrea C.
|
| ISBN |
9781607615941 |
|
1607615940 |
|
