Limit search to available items
Book Cover
E-book

Title Adhesion protein protocols / edited by Amanda S. Coutts
Edition 3rd ed
Published New York : Humana Press, 2013

Copies

Description 1 online resource (xii, 407 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; v. 1046
Methods in molecular biology (Clifton, N.J.) ; volume 1046. 1064-3745
Contents Reconstructing Integrin Activation in vitro -- Quantifying Cellular Adhesion to Covalently Immobilized Extracellular Matrix Proteins by Single-Force Spectroscopy -- A Multimode-TIRFM & Microfluidic Technique to Examine Platelet Adhesion Dynamics -- Localization-Based Super-Resolution Imaging of Cellular Structures -- Use of Microarray Analysis to Investigate EMT Gene Signatures.- Podosome Reformation in Macrophages: Assays and Analysis -- Mobile and Three-Dimensional Presentation of Adhesion Proteins Within Microwells -- Basement Membrane Invasion Assays: Native Basement Membrane and Chemoinvasion Assay -- Cytoplasmic Actin: Purification and Single Molecule Assembly Assays -- Synthetic and Tissue-Derived Models for Studying Rigidity Effects on Invadopodia Activity -- Determining Rho GTPase Activity by an Affinity-Precipitation Assay -- Proteomic and Biochemical Methods to Study the Cytoskeletome. Fabricating Surfaces with Distinct Geometries and Different Combinations of Cell Adhesion Proteins -- Purification of Native Arp2/3 Complex from Bovine Thymus -- Purification of Arp2/3 Complex from Saccharomyces Cerevisiae -- Measurement and Analysis of in vitro Actin Polymerization -- Use of the xCELLigence System for Real Time Analysis of changes in Cellular Motility and Adhesion in Physiological Conditions -- Measuring Chemotaxis Using Direct Visualisation Microscope Chambers -- In Vitro Microtubule Severing Assays -- VE-Cadherin Status as an Indicator of Microvascular Permeability -- High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis -- Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells -- Electrospun Nanofiber Scaffolds for Investigating Cell-matrix Adhesion -- Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein-Membrane Interactions -- VE-Cadherin Status as an Indicator of Microvascular Permeability -- High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis. Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells -- Electrospun Nanofiber Scaffolds for Investigating Cell-matrix Adhesion -- Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein-Membrane Interactions
Summary Cellular adhesion is a fundamental process that influences numerous biological activities such as morphogenesis, cell motility and division, as well as signalling. In addition, adhesion is a process important not only in normal physiology and development, but also in disease states such as tumourigenesis, cardiovascular disease, inflammation and infection. There are a plethora of proteins involved in adhesion-related events with a huge diversity in function. As a result, a wide variety of techniques exist to study adhesion related proteins and processes. In Adhesion Protein Protocols, Third Edition, chapters cover techniques to gain insight into the complex and incompletely understood processes that are involved in cellular adhesion. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Adhesion Protein Protocols, Third Edition will be useful for both those new to the field of adhesion protein research as well as the more experienced scientist
Analysis Life sciences
Biochemistry
Cytology
Protein Science
Cell Physiology
eiwitten
proteins
adhesie
adhesion
levenswetenschappen
celfysiologie
laboratoriummethoden
laboratory methods
protocollen
protocols
Proteins and Enzymes
Biological Techniques
Eiwitten en enzymen
Biologische technieken
Bibliography Includes bibliographical references and index
Notes English
Online resource; title from PDF title page (SpringerLink, viewed July 30, 2013)
Subject Cell adhesion molecules -- Laboratory manuals
Glycoproteins.
Cell physiology.
Cell surface antigens.
Antigens.
Membrane proteins.
Proteins.
Biological products.
Biologicals.
Cell adhesion molecules.
Cell adhesion.
Membrane Glycoproteins
Cell Physiological Phenomena
Publication Formats
Antigens, Surface
Antigens
Membrane Proteins
Publication Characteristics
Glycoproteins
Proteins
Biological Factors
Phenomena and Processes
Chemicals and Drugs
Amino Acids, Peptides, and Proteins
Laboratory Manuals
Cell Adhesion Molecules
Cell Adhesion
Biological Products
protein.
Proteins
Membrane proteins
Glycoproteins
Cell surface antigens
Cell physiology
Cell adhesion
Biologicals
Biological products
Antigens
Cell adhesion molecules
Human Anatomy & Physiology.
Health & Biological Sciences.
Animal Biochemistry.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Coutts, Amanda S.
ISBN 9781627035385
1627035389
1627035370
9781627035378