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Title Peptide antibodies : methods and protocols / edited by Gunnar Houen
Published New York : Humana Press : Springer, [2015]


Description 1 online resource (xiv, 366 pages) : color illustrations
Series Methods in molecular biology, 1940-6029 ; volume 1348
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 1348. 1064-3745
Springer protocols (Series)
Contents Peptide Antibodies: Past, Present, and Future / Gunnar Houen -- Structure of natural and recombinant antibodies / Hui Ma and Richard O'Kennedy -- Prediction of antigenic B and T cell epitopes via energy decomposition analysis : description of the web-based prediction tool BEPPE / Claudio Peri [and others] -- Prediction of antibody epitopes / Morten Nielsen and Paolo Marcatili -- Fmoc solid-phase peptide synthesis / Paul R. Hansen and Alberto Oddo -- Peptide-carrier conjugation / Paul R. Hansen -- Solid-phase peptide-carrier conjugation / Gunnar Houen and Dorthe T. Olsen -- Analysis of peptides and conjugates by amino acid analysis / Peter Højrup -- Characterization of synthetic peptides by mass spectrometry / Bala K. Prabhala [and others] -- Interpretation of tandem mass spectrometry (MSMS) spectra for peptide analysis / Karin Hjernø and Peter Højrup -- Polyclonal Peptide Antisera / Tina H. Pihl, Kristin E. Illigen, and Gunnar Houen -- Production and screening of monoclonal peptide antibodies / Nicole Hartwig Trier [and others] -- Production of epitope-specific antibodies by immunization with synthetic epitope peptide formulated with CpG-DNA- liposome complex without carriers / Dongbum Kim, Younghee Lee, and Hyung-Joo Kwon -- Thioredoxin-displayed multipeptide immunogens / Angelo Bolchi [and others] -- Purification of natural and recombinant peptide antibodies by affinity chromatographic strategies / Hui Ma and Richard O'Kennedy -- Isolation of camelid single-domain antibodies against native proteins using recombinant multivalent peptide ligands / Norah A. Alturki [and others] -- Generation of TCR-Like antibodies using phage display / Brian H. Santich [and others] -- Structural characterization of peptide antibodies/ Anna Chailyan and Paolo Marcatili -- Automated high-throughput mapping of linear B-cell epitopes using a statistical analysis of high-density peptide microarray data / Thomas Østerbye and Søren Buus -- Characterization of peptide antibodies by epitope mapping using resin-bound and soluble peptides / Nicole Hartwig Trier -- Screening and characterization of linear B-cell epitopes by biotinylated peptide libraries / Ida Rosenkrands and Anja Olsen -- Bead-based peptide arrays for profiling the specificity of modification state-specific antibodies / Angela Filomena [and others] -- Surface plasmon resonance method to evaluate anti-citrullinated protein/peptide antibody affinity to citrullinated peptides / Feliciana Real-Fernández [and others] -- Specificity analysis of histone modification-specific antibodies or reading domains on histone peptide arrays / Goran Kungulovski [and others] -- Prion-specific antibodies produced in wild-type mice / Peter M.H. Heegaard [and others] -- Immunoblotting with peptide antibodies : differential immunoreactivities caused by certain amino acid substitutions in a short peptide and possible effects of differential refolding of the peptide on a nitrocellulose or PVDF membrane / Takenori Yamamoto [and others] -- Immunocytochemical and immunohistochemical staining with peptide antibodies / Tina Friis [and others] -- Designing B-cell epitopes for immunotherapy and subunit vaccines / Harinder Singh [and others] -- Enterovirus-specific anti-peptide antibodies / Chit Laa Poh [and others] -- Therapeutic HIV peptide vaccine / Anders Fomsgaard
Summary This extensive volume covers basic and advanced aspects of peptide antibody production, characterization and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on specific posttranslational modifications are of great interest, such as phosphorylation, citrullination and others, while different forms of recombinant peptide antibodies are gaining interest, notably nanobodies, single chain antibodies, TCR-like antibodies, among others. Within this volume, those areas are covered, as well as several technical and scientific advances
Bibliography Includes bibliographical references and index
Subject Peptides -- Laboratory manuals
Immunoglobulins -- Laboratory manuals
Antigenic determinants -- Laboratory manuals
Peptides -- immunology
Medical -- Immunology.
Antigenic determinants
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Houen, Gunnar, 1955- editor.
ISBN 9781493929993