Title Antibody engineering. Volume 1 / Roland Kontermann, Stefan Dubel (eds.)

Edition 2nd ed Published Berlin ; London : Springer, 2010

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Description 1 online resource (viii, 788 pages) Series Springer Protocols Handbooks, 1949-2448 Springer protocols (Series) Contents Part 1. Cloning of the antigen-binding site from hybridoma: Cloning of variable domains from mouse hybridoma by PCR -- Coning hybridoma cDNA by RACE -- Construction of scFv fragments from hybridoma or spleen cells by PCR assembly -- Part 2: Generation of antibody repertoires -- Mouse immune libraries for the generation of scFv fragments directed against human cell surface antigens -- Human antibody gene libraries -- Synthetic antibody Libraries -- Immune libraries from nonhuman primates (NHP) -- Generation of rabbit immune libraries -- Part III: Selection of antibody fragments from combinatorial libraries -- Immunotube selections -- Phage display and selection in microtitre plates -- Phage display and selections on biotinylated antigens -- Phage display and subtractive selection on cells -- Selection of phage antibody libraries for binding and internalization into mammalian cells -- Improving phage display throughput by using hyperphage, miniaturized titration and pVIII (g8p) ELISA -- yeast display and selections -- The generation of transgenic mice expressing human antibody repertoires -- Selection of antibody fragments by means of the gilter-sandwich colony screening assay -- Semi-automated magnetic bead-based antibody selection from phage display libraries -- Part IV: Engineering and production of immunoglobulins -- Aspects of isotype selection -- Generation of heavy and light chains (chimeric antibodies) -- Humanising antibodies by CDR grafting -- Humanization by resurfacing -- Human antibodies by guided selection -- In silico de-immunization -- Affinity maturation by chain shuffling and site directed mutagenesis -- Affinity maturation by random mutagenesis and phage display -- Engineering of the Fc Region for Improved PK (FcRn Interaction) -- Antibody-dependent Enzyme Prodrug Therapy (ADEPT) -- Production of recombinant human IgG antibodies in the baculovirus expression system -- Expression of IgA molecules in mammalian cells -- Part V: Antibody characterization -- Expression of complete antibodies in transgenic plants -- Expression of full length monoclonal antibodies (mAb) in algal chloroplast -- Expression of IgG Antibodies in mammalian cells -- Protein A/G chromatography -- Epitope analysis using synthetic peptide repertoires prepared by SPOT synthesis technology -- Epitope mapping by printed peptide libraries -- Antibody epitope mapping using yeast display -- Size exclusion chromatography -- Structural characterization of antibodies by mass spectrometry -- Antibody glycans characterization -- Affinity measurements by competition ELISA -- Anti-histidine antibodies as tools for reversible capturing of His-tagged fusion proteins for subsequent binding analysis -- Affinity measurements using quartz crystal microbalance (QCM) -- Affinity measurements with radiolabeled antibodies -- Neutralization tests -- Functional characterization of antibodies neutralizing soluble factors in vitro and in vivo -- Competitive ELISA -- Quantification of human IgG and related Fc fusion proteins by a human IgG/Fc capture ELISA -- Determination of Fc-mediated antibody-effector functions by chromium release assay -- Binding studies with flow cytometry -- Index Summary This book is a toolbox of well-established basics and many exciting new techniques in antibody engineering, reflecting the latest hands-on work of key laboratories in this fast-moving field. The new edition is expanded to nearly double the number of protocols Analysis eiwitten proteins geneeskunde medicine celbiologie cellular biology antilichamen antibodies biologische technieken biological techniques biomedische wetenschappen biomedicine Medicine (General) Geneeskunde (algemeen) Notes Previous edition: published in one volume. 2001 Bibliography Includes bibliographical references and index Notes English Print version record Subject Recombinant antibodies -- Laboratory manuals Antibodies -- genetics DNA, Recombinant -- immunology Antibody Formation -- genetics Genetic Engineering -- methods Biomédecine. Sciences de la vie. Recombinant antibodies Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Kontermann, Roland, 1961- Dübel, Stefan. ISBN 9783642011443 3642011446 1282830546 9781282830547 9786612830549 6612830549 9783642011450 3642011454 9783662495940 3662495945

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