| Description |
1 online resource (xii, 589 pages) : illustrations (some color) |
| Contents |
Part I. Bioinformatics of Antigen-binding Sites -- 1. Analysis of Single Chain Antibody Sequences Using the VBASE2 Fab Analysis Tool / Svetlana Mollova, Ida Retter, Michael Hust, Stefan Dübel, and Werner Müller -- 2. Standardized Sequence and Structure Analysis of Antibody Using IMGT1 / François Ehrenmann, Patrice Duroux, Véronique Giudicelli, and Marie-Paule Lefranc -- 3. Protein Sequence and Structure Analysis of Antibody Variable Domains / Andrew C.R. Martin -- Part II. Generation of Antibody Fragments and Their Derivatives -- 4. scFv by Two-Step Cloning / Dafne Müller -- 5. Bivalent Diabodies / Roland E. Kontermann -- 6. Generation of Single-Chain Fv Fragments and Multivalent Derivatives scFv-Fc and scFv-CH3 (Minibodies) / Tove Olafsen, Vania E. Kenanova, and Anna M. Wu -- 7. Miniantibodies / Jonas V. Schaefer, Peter Lindner, and Andreas Plückthun -- 8. Generation of Stably Transfected Eukaryotic Cell Lines Producing ImmunoRNAse Fusion Proteins / Athanasios Mavratzas, Evelyn Exner, Jürgen Krauss, and Michaela A.E. Arndt -- 9. Antibody-Cytokine Fusion Proteins with Members of the TNF-Family / Dafne Müller and Jeannette Gerspach -- 10. Recombinant Immunotoxins for Treating Cancer / Ira Pastan and Mitchell Ho -- 11. T Bodies / Bianca Altvater, Silke Landmeier, and Claudia Rossig -- 12. Expressing Intrabodies in Mammalian Cells / Alessio Cardinale and Silvia Biocca -- 13. Phenotypic Knockdown with Intrabodies / Nina Strebe and Manuela Schüngel -- 14. Disulfide-Stabilized Fv Fragments / Ulrich Brinkmann -- 15. PEGylation of Antibody Fragments to Improve Pharmacodynamics and Pharmacokinetics / Arutselvan Natarajan and Sally J. DeNardo -- 16. Fusion Proteins with Improved PK / Roland Stork -- 17. In Vivo Biotinylated scFv Fragments / Laila Al-Halabi and Torsten Meyer -- 18. Bispecific Diabodies and Single-Chain Diabodies / Roland E. Kontermann -- 19. Generation and Characterization of a Dual Variable Domain Immunoglobulin (DVD-IgTM) Molecule / Chengbin Wu, Tariq Ghayur, and Jochen Salfeld -- 20. Isolation of Antigen-Specific Nanobodies / Gholamreza Hassanzadeh Ghassabeh, Dirk Saerens, and Serge Muyldermans -- 21. CDR-FR Peptides / Xiao-Qing Qiu -- Part III. Production of Antibody Fragments -- 22. Purification and Characterization of His-Tagged Antibody Fragments / Martin Schlapschy, Markus Fiedler, and Arne Skerra -- 23. Production of Antibody Fragments in the Gram-Positive Bacterium Bacillus megaterium / Miriam Steinwand, Eva Jordan, and Michael Hust -- 24. Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L / Remko Griep and John McDougall -- 25. Purification and Analysis of Strep-tagged Antibody-Fragments / Martin Schlapschy and Arne Skerra -- 26. Production of Antibodies and Antibody Fragments in Escherichia coli / Dorothea E. Reilly and Daniel G. Yansura -- 27. Improving Expression of scFv Fragments by Co-expression of Periplasmic Chaperones / Jonas V. Schaefer and Andreas Plückthun -- 28. Bioreactor Production of scFv Fragments in Pichia pastoris / Stephan Hellwig and Georg Melmer -- 29. Expression of Antibody Fragments in Transgenic Plants / Udo Conrad and Doreen M. Floss -- 30. Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells / Thomas Schirrmann and Konrad Büssow -- Part IV. Recombinant Antibody Molecules in Nanobiotechnology and Proteomics -- 31. Immunoliposomes / Sylvia K.E. Messerschmidt, Julia Beuttler, and Miriam Rothdiener -- 32. Targeted Polymeric Nanoparticles / Katharina Landfester and Anna Musyanovych -- 33. Antibody Microarrays for Expression Analysis / Christoph Schröder, Anette Jacob, Sven Rüffer, Kurt Fellenberg, and Jörg D. Hoheisel -- 34. Evaluation of Recombinant Antibodies on Protein Microarrays Applying the Multiple Spotting Technique / Zoltán Konthur and Jeannine Wilde -- Part V. Preclinical and Clinical Development -- 35. Xenograft Mouse Models for Tumour Targeting / Colin Green, Hakim Djeha, Gail Rowlinson-Busza, Christina Kousparou, and Agamemnon A. Epenetos -- 36. Xenograft Mouse Models for Tumour Targeting / Surinder K. Sharma and R. Barbara Pedley -- 37. Imaging Tumor Xenografts Using Radiolabeled Antibodies / Tove Olafsen, Vania E. Kenanova, and Anna M. Wu -- 38. Human Anti-antibody Response / Natalie L. Griffin, Hassan Shahbakhti, and Surinder K. Sharma -- 39. IP Issues in the Therapeutic Antibody Industry / Ulrich Storz and Alan J. Morrison -- Appendix: Amino Acids and Codons |
| Summary |
Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. ""Antibody Engineering"" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest 'hands on' knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include help |
| Analysis |
geneeskunde |
|
medicine |
|
biologische technieken |
|
biological techniques |
|
celbiologie |
|
cellular biology |
|
eiwitten |
|
proteins |
|
antilichamen |
|
antibodies |
|
biomedische wetenschappen |
|
biomedicine |
|
Medicine (General) |
|
Geneeskunde (algemeen) |
| Notes |
Previous edition: published in one volume. 2001 |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Recombinant antibodies -- Laboratory manuals
|
|
Antibodies -- genetics
|
|
DNA, Recombinant -- immunology
|
|
Antibody Formation -- genetics
|
|
Genetic Engineering -- methods
|
|
Biomédecine.
|
|
Sciences de la vie.
|
|
Recombinant antibodies
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Kontermann, Roland, 1961-
|
|
Dübel, Stefan.
|
| ISBN |
9783642011474 |
|
3642011470 |
|
3642011462 |
|
9783642011467 |
|
1282830538 |
|
9781282830530 |
|
9786612830532 |
|
6612830530 |
|
