Title Antibody-drug conjugates / edited by Laurent Ducry

Published New York : Humana Press, 2013

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Description 1 online resource (x, 317 pages) : illustrations Series Methods in Molecular Biology, 1064-3745 ; 1045 Methods in molecular biology (Clifton, N.J.) ; v. 1045. 1064-3745 Contents Antibody-Drug Conjugate (ADC) Clinical Pipeline: A Review -- Antibody-Drug Conjugate Target Selection: Critical Factors -- Selecting an Optimal Antibody for Antibody-Drug Conjugate Therapy: Internalization and Intracellular Localization -- Antibody-Drug Conjugate Payloads -- Linker Technologies for Antibody-Drug Conjugates -- In Vivo Testing of Drug-Linker Stability -- Pharmacokinetics and ADME Characterizations of Antibody-Drug Conjugates -- Safe Handling of Cytotoxic Compounds in a Biopharmaceutical Environment -- Micro- and Mid-Scale Maleimide-Based Conjugation of Cytotoxic Drugs to Antibody Hinge Region Thiols for Tumor Targeting -- Protocols for Lysine Conjugation -- Engineering THIOMABs for Site-Specific Conjugation of Thiol-Reactive Linkers -- Enzymatic Antibody Modification by Bacterial Transglutaminase -- Formulation Development of Antibody-Drug Conjugates -- Conjugation Process Development and Scale-Up -- Methods for Conjugating Antibodies to Nanocarriers -- Drug-to-Antibody Ratio (DAR) by UV/Vis Spectroscopy -- Drug-to-Antibody Ratio (DAR) and Drug Load Distribution by Hydrophobic Interaction Chromatography and Reversed Phase High Performance Liquid Chromatography -- Drug-to-Antibody Ratio (DAR) and Drug Load Distribution by LC-ESI-MS -- Determination of Charge Heterogeneity and Level of Unconjugated Antibody by Imaged cIEF -- Risk-Based Scientific Approach for Determination of Extractables/Leachables from Biomanufacturing of Antibody-Drug Conjugates (ADCs) Summary Antibody-drug conjugates (ADCs) represent a promising therapeutic approach for cancer patients by combining the antigen-targeting specificity of monoclonal antibodies (mAbs) with the cytotoxic potency of chemotherapeutic drugs. In Antibody-Drug Conjugates, expert researchers provide detailed protocols for many of the key ADC techniques necessary for working in the field. These chapters and methodologies are aimed at the key tasks necessary to identify a suitable target, properly design the mAb, the linker and the payload, as well as to conjugate them in a reproducible and scalable fashion. Written in the highly successful Methods in Molecular Biology format, these detailed chapters include the kind of practical implementation advice that guarantees quality results. Authoritative and timely, Antibody-Drug Conjugates aims to further drive ADC development and thus help toward improving cancer treatments of the future Analysis farmacologie pharmacology toxicologie toxicology biomedische wetenschappen biomedicine oncologie oncology Medicine (General) Geneeskunde (algemeen) Notes Includes index Bibliography Includes bibliographical references and index Notes English Online resource; title from PDF title page (SpringerLink, viewed August 20, 2013) Subject Antibody-drug conjugates -- Laboratory manuals Antibody-toxin conjugates -- Laboratory manuals Immunoglobulins. Diseases. Blood proteins. Globulins. Proteins. Monoclonal antibodies. Antibody-toxin conjugates. Drug interactions. Drugs. Immunoglobulins Disease Chemicals and Drugs Therapeutic Uses Immunoproteins Pharmacologic Actions Chemical Actions and Uses Serum Globulins Blood Proteins Globulins Proteins Amino Acids, Peptides, and Proteins Neoplasms Antibodies, Monoclonal Antineoplastic Agents Antibodies Immunoconjugates Drug Combinations Pharmaceutical Preparations protein. Proteins Monoclonal antibodies Immunoglobulins Globulins Drugs Drug interactions Diseases Blood proteins Antibody-drug conjugates Antibody-toxin conjugates Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Ducry, Laurent, editor. ISBN 9781627035415 1627035419 1627035400 9781627035408 1927035414 9781927035412

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