| Description |
1 online resource (394 pages, 2 unnumbered pages of plates) : illustrations (some color) |
| Contents |
Antibodies; Matthew R. Kaser and Gary C. Howard; ; Antigens; Paul Algate, Jory Baldridge, and Sally Mossman; ; Adjuvants; Jory Baldridge, Paul Algate, and Sally Mossman; ; Production of Polyclonal Antibodies; Lon V. Kendall; ; Production of Monoclonal Antibodies; Kathleen C.F. Sheehan; ; Quantitative Production of Monoclonal Antibodies; David A. Fox and Elizabeth M. Smith; ; Purification and Characterization of Antibodies; Joseph P; Chandler; ; Making Antibodies in Bacteria; Frederic A. Fellouse and Sachdev S. Sidhu; ; Chemical and Proteolytic Modification of Antibodies; George P. Smith; ; Applications; Lee Bendickson and Marit Nilsen-Hamilton; ; Immunohistochemical Methods; José A. Ramos-Vara and Julie Ackerman Saettele; ; Immunoelectron Microscopy; Sara E. Miller and David N. Howell; ; Flow Cytometry; Kristi R. Harkins and M. Elaine Kunze; ; ELISAs; John Chen and Gary C. Howard; ; Antibodies in the Future: Challenges and Opportunities; Matthew R. Kaser and Gary C. Howard |
| Summary |
Written for professionals in biomedical research, Making and Using Antibodies: A Practical Handbook presents methods for the production, characterization, and use of antibodies. It takes readers step-by-step from antigen preparation to immunizations, adjuvants, screening, purification, storage, and application. Introductory material preceding each chapter gives readers the necessary background. It covers basic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Immunoglobulins -- Handbooks, manuals, etc
|
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Immunoglobulins.
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Medical laboratory technology.
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Antibody Formation
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Antibodies
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Clinical Laboratory Techniques
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SCIENCE -- Life Sciences -- Anatomy & Physiology.
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Medical laboratory technology
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Immunoglobulins
|
| Genre/Form |
Laboratory manuals
|
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Handbooks and manuals
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Laboratory manuals.
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Howard, Gary C
|
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Kaser, Matthew R
|
| LC no. |
2006047535 |
| ISBN |
9781420005196 |
|
1420005197 |
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1280732881 |
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9781280732881 |
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9780429186899 |
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0429186894 |
|
