| Description |
1 online resource (xi, 364 pages) : illustrations |
| Series |
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1060 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1060. 1064-3745
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| Contents |
Human monoclonal antibodies : the residual challenge of antibody immunogenicity / Herman Waldmann -- Technical and ethical limitations in making human monoclonal antibodies (an overview) / Mark C. Glassy and Rishab Gupta -- Therapeutic human monoclonal antibodies in inflammatory diseases / Sotirios Kotsovilis and Evangelos Andreakos -- Therapeutic human monoclonal antibodies against cancer / Jamie Jarboe, Anumeha Gupta, and Wasif Saif -- Polyclonal and monoclonal antibodies in clinic / Bharath Wootla, Aleksandar Denic, and Moses Rodriguez -- Production of human monoclonal Antibodies by the Epstein-Barr virus method / Michael Steinitz -- Humanization and simultaneous optimization of monoclonal antibody / T. Kuramochi, T. Igawa, H. Tsunoda, and K. Hattori -- Chimeric Antibodies / Kohei Kurosawa, Waka Lin, and Kunihiro Ohta -- Recombinant genetic libraries and human monoclonal antibodies / Jarrett J. Adams, Bryce Nelson, and Sachdev S. Sidhu -- Production of stabilized scFv antibody fragments in the E. coli bacterial cytoplasm / Lilach Vaks and Itai Benhar -- Construction and production of an IgG-like tetravalent bispecific antibody, IgG-single-chain Fv fusion / Dan Lu and Zhenping Zhu -- Construction of human antibody gene libraries and selection of antibodies by phage display / André Frenzel [and others] -- Antigen-specific human monoclonal antibodies from transgenic mice / Susana Magadán Mompó and África González-Fernández -- Phage display technology for human monoclonal antibodies / Cecilia Deantonio [and others] -- Antigen-specific in vitro immunization : a source for human monoclonal antibodies / Kosuke Tomimatsu and Sanetaka Shirahata -- Methods for radiolabelling of monoclonal antibodies / Vladimir Tolmachev, Anna Orlova, and Karl Andersson -- Purification of human monoclonal antibodies and their fragments / Thomas Müller-Späth and Massimo Morbidelli -- Idiotype-specific intravenous immunoglobulin (IVIG) for therapy of autoimmune diseases / Miri Blank, Tomer Bashi, and Yehuda Shoenfeld |
| Summary |
The introduction of monoclonal antibodies revolutionized immunology. The development of human monoclonal antibodies was inspired primarily by the enormous clinical benefits promised by these reagents which can be used as anti-inflammatory reagents, anti-tumor reagents and reagents for passive immunization in a variety of pathologies. Human Monoclonal Antibodies: Methods and Protocols presents technical protocols of cellular and molecular methods for the production, purification and application of human monoclonal antibodies, as well as review articles on related topics of human monoclonal and polyclonal antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Human Monoclonal Antibodies: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies which will prove invaluable in a clinical setting |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed September 16, 2013) |
| Subject |
Monoclonal antibodies -- Laboratory manuals
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Monoclonal antibodies.
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Human beings.
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Humans
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Antibodies, Monoclonal
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Homo sapiens (species)
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Monoclonal antibodies
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Steinitz, Michael (Immunologist), editor.
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| ISBN |
9781627035866 |
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1627035869 |
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1627035850 |
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9781627035859 |
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