| Description |
1 online resource (x, 267 pages) : illustrations |
| Series |
Methods in molecular medicine ; 46 |
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Methods in molecular medicine ; 46. 1543-1894
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| Contents |
Therapeutic inhibition of angiogenesis / Hua-Tang Zhang and Roy Bicknell -- Microscopic assessment of angiogenesis in tumors / Stephen B. Fox -- Vivo matrigel migration and angiogenesis essays / Katherine M. Malinda -- Alginate microbead release assay of angiogenesis / Clifford Y. Ko [and others] -- DISC angiogenesis essay / Anthony C. Allison and Luis-F. Fajardo -- Sponge implant model of angiogenesis / Silvia P. Andrade -- Hollow fiber essay for tumor angiogenesis / Roger M. Phillips and Michael C. Bibby -- Dorsal skinfold chamber preparation in mice: studying angiogenesis by intravital microscopy / Axel Sckell and Michael Leunig -- Angiogenesis essays using chick chorioallantoic membrane / David C. West [and others] -- Corneal essay for angiogenesis / Marina Ziche -- Collagen gel essay for angiogenesis: induction of endothelial cell sprouting / Ana M. Schor, Ian Ellis, and Seth L. Schor -- Chemotaxis and chemokinesis in 3D macromolecular matrices: relevance to angiogenesis / Ana M. Schor, Ian Ellis, and Seth L. Schor -- Rat aortic ring: 3D model of angiogenesis in vitro / Mike F. Burbridge and David C. West -- In vitro matrigel angiogenesis essays / M. Lourdes Ponce -- Microvessel endothelial cells from human adipose tissues: isolation, identification, and culture / Peter W. Hewett -- Transfection and transduction of primary human endothelial cells / Stewart G. Martin -- Vascular smooth muscle cells: isolation, culture, and characterization / Richard C.M. Siow and Jeremy D. Pearson -- Bovine retinal microvascular pericytes: isolation, propagation, and identification / Ramesh C. Nayak and Ira M. Herman |
| Summary |
Although angiogenesis-the formation of new blood vessels from pre-existing ones-has emerged as an exciting new target for the treatment of many varied disorders, fundamental aspects of this important biological process remain poorly understood. To address the needs of those working in such a dramatically advancing area, Cliff Murray and a panel of experienced investigators have brought together in Angiogenesis Protocols a comprehensive collection of key techniques for assessing the formation of new blood vessels in both whole tissues and cell culture. These readily reproducible methods range from low-cost in vitro assays to cutting-edge in vivo models, such as the dorsal window chamber, which demands high levels of surgical skill as well as relatively expensive, custom-made equipment. The authors have also included details of the associated techniques for the isolation, identification, and culture of microvessel endothelial cells from human adipose tissues and for the transfection and transduction of primary human endothelial cells. Timely and highly practical, Angiogenesis Protocols is a gold-standard resource for today's angiogenesis investigator, providing easy access to a wide variety of proven assays suitable for laboratories with greatly differing technical expertise, materials, and financial resources |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| Subject |
Neovascularization -- Laboratory manuals
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Blood-vessels -- Growth -- Molecular aspects -- Laboratory manuals
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Neovascularization.
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Angiogenesis Inducing Agents -- analysis
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Biological Assay -- methods
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Neovascularization, Pathologic
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MEDICAL -- Physiology.
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SCIENCE -- Life Sciences -- Human Anatomy & Physiology.
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Neovascularization
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Wetenschappelijke technieken.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Murray, J. Clifford.
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| ISBN |
9781592591435 |
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1592591434 |
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0896036987 |
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9780896036987 |
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